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香稜丸이 子宮頸部癌細胞(HeLa Cell)의 apoptosis에 미치는 影響

Hangreunghwan water extract induced apoptosis in Human Cervical carcinoma HeLa cells.

박종규|김송백|조한백|유심근
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2004.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제17권 제2호|pp. 11-26

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참고문헌

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초록

Abstract

To address the ability of Hangreunghwan(HRH) to induce cell death, we investigated the effect of HRH on cell viability. Twenty-four hours later, loss of viability occurred following HRH exposure in a dose-dependent manner. The treatment of HRH, a commonly used herb formulation in Korea, Japan and China, caused a decrease in cell viability. HRH also resulted in apoptotic morphology a brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining, and reduction of cell volume. Our results show that 2mg/ml HRH induces mitochondria membrane potential collapse. Immunoblotting data also shows that the expression of Bcl-2, antiapoptotic protein, decrease by the addition of HRH. This GFP-Bax overexpression system shows that an important pro-apoptotic Bcl-2-family protein, Bax is translocated to mitochondria by the addition of 2 mg/ml HRH. Interestingly, the treatment of BAPTA-AM, calcium chelator, shows that calcium is involved in HRH-induced apoptosis including caspase-3 activation in HeLa cells. BAPTA-AM reverses HRH-induced inhibition of XIAP expression. In addition, the calcium chelator inhibits the increase of Bax expression which is induced by HRH. However, the treatment of BAPTA-AM does not have any effect on HRH-induced Bax translocation. In conclusion, calcium is involved in both HRH-increased Bax expression and HRH-regulated XIAP expression, resulting in apoptosis in HeLa human cervical carcinoma cells.

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