치과대학 대학생 타액의 치아 우식활성도 평가(I)
A Study on Dental Caries Activity Assessment from Saliva of Students of Dentistry College (I)
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Composition of oral microbial flora is suggested to play a role in the maintenance of oral health. Among them, Streptococcus mutans plays an essential role in the formation of dental plaque and it is being noticed as incipient infective bacteria of dental caries. The purpose of this study was to prepare the basic data about distribution of Streptococcus mutans detected from saliva by measuring colony density. It is well known that smoking as well as drinking is a factor of dental caries, however there are few investigations about distribution of Streptococcus mutans either smoking or drinking. The materials of this study were collected from students of dentistry college with general characteristics obtained by self-administered questionnaires. Students were asked to gently chew the paraffin to get saliva for 1 minute. Bacitracin disc in the culture medium was activated for 15 minutes, and then sample was incubated in the activated medium at 37oC incubator for 48 hours. Streptococcus mutans colonization per 1 ml saliva was measured by the number of criteria. The severe levels of dental caries activity were significantly increased in the case of smoking (from 5.6% to 10%) or drinking (form 0% to 7%), respectively. Also, dental caries activity increased according to smoking and drinking periods longer. However, using the SPSS analytical process (Mann-Whitney's U), no significant differences were observed between the smoking and the non-smoking or the drinking and non-drinking, respectively. These results obtained from dental caries activity of saliva suggest that distribution of Streptococcus mutans following smoking or drinking can be used as a significant information for oral health.
Abstract
Composition of oral microbial flora is suggested to play a role in the maintenance of oral health. Among them, Streptococcus mutans plays an essential role in the formation of dental plaque and it is being noticed as incipient infective bacteria of dental caries. The purpose of this study was to prepare the basic data about distribution of Streptococcus mutans detected from saliva by measuring colony density. It is well known that smoking as well as drinking is a factor of dental caries, however there are few investigations about distribution of Streptococcus mutans either smoking or drinking. The materials of this study were collected from students of dentistry college with general characteristics obtained by self-administered questionnaires. Students were asked to gently chew the paraffin to get saliva for 1 minute. Bacitracin disc in the culture medium was activated for 15 minutes, and then sample was incubated in the activated medium at 37oC incubator for 48 hours. Streptococcus mutans colonization per 1 ml saliva was measured by the number of criteria. The severe levels of dental caries activity were significantly increased in the case of smoking (from 5.6% to 10%) or drinking (form 0% to 7%), respectively. Also, dental caries activity increased according to smoking and drinking periods longer. However, using the SPSS analytical process (Mann-Whitney's U), no significant differences were observed between the smoking and the non-smoking or the drinking and non-drinking, respectively. These results obtained from dental caries activity of saliva suggest that distribution of Streptococcus mutans following smoking or drinking can be used as a significant information for oral health.
참고문헌 (21)
- Allaker RP, "Novel anti-microbial therapies for dental plaque-related diseases", Int J Antimicrob Agents, 33, pp.8~13 (2009)
- Shin HS, "In-depth analyses of the third national health and nutrition examination survey, the health interview and health attitude survey part", Korea Centers for Disease Control and Prevention April, Seoul, Republic of Korea, ~ (2007)
- Koga T, "Sucrose dependent cell adherence and cariogenicity of serotype c Streptococcus mutans", J Gen Microbiol, 132, pp.2873~2883 (1986)
- Hamada S, "Biology, immunology, and cariogenicity of Streptococcus mutans", Microbiol Rev, 44, pp.331~384 (1980)
- 윤요한, "Identification of Inhibitory Effect on Streptococcus mutans by Oleanolic Acid", 생명과학회지, 20(3), pp.321~325 (2010), 한국생명과학회
- Streckfus CF, "Saliva as a diagnostic fluid", Oral Dis, 8, pp.69~76 (2002)
- Wenham DG, "Insoluble glucan synthesis by mutansucrase as determinant of the cariogenicity of Streptococcus mutans", J Gen Microbiol, 127, pp.407~415 (1981)
- Inoue M, "Synthesis adherent insoluble glucan by the concerted action of the two glucosyltranferase components of Streptococcus mutans", FEBS Lett, 143, pp.101~104 (1982)
- "Dental biofilm, Biochem Mol Biol News" (2008)[URL]
- Lawrence HP, "Salivary markers of systemic disease: noninvasive diagnosis of disease and monitoring of general health", J Can Dent Assoc, 68, pp.170~174 (2002)
- Kaufman E, "The diagnostic applications of saliva-a review", Crit Rev Oral Biol Med, 13, pp.197~212 (2002)
- Lee JI, "Correlation co-efficiency between the caries incidence and the periodontal status in Korea", 석사, pp.2005
- Sayegh A, "Food and drink consumption, sociodemographic factors and dental caries in 4∼5- year-old children in Amman", Jordan. Br Dent, 193, pp.37~42 (2002)
- Bracquart P, "An agar medium for the differential enumeration of Streptococcus thermophilus and Lactobacillus bulgaricus in yoghurt", J Appl Bacteriol, 51, pp.303~305 (1981)
- de MAN JC, "A medium for the cultivation of lactobacilli", J Appl Bacteriol, 23, pp.130~135 (1960)
- Matsumoto Y, "A rapid and quantitative detection system for Streptococcus mutans in saliva using monoclonal antibodies", Caries Res, 40, pp.15~19 (2006)
- Everhart DL, "The determination of antibody to Streptococcus mutans serotypes in saliva for children ages 3 to 7 years", J Dent Res, 57, pp.631~635 (1978)
- Han GS, "Periodontal status in smokers and the effects of professional periodontal care", 박사, pp.2007
- Ge Y, "Streptococcus mutans and Streptococcus sanguinis colonization correlated with caries experience in children", Caries Res, 42, pp.444~448 (2008)
- 정현자, "흡연자와 비흡연자간의 구강 내 세균 분포 및 항균제 감수성", 미생물학회지, 46(4), pp.334~340 (2010), 한국미생물학회