Effects of Bamboo Salt with Sodium Fluoride on the Prevention of Dental Caries
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria. Methods: The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe’s post-hoc test. The Kruskal–Wallis test was used to compare adhesion, followed by the Mann–Whitney test. Gene expression in the experimental and control samples was compared using the Student’s t-test. Results: Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups. Conclusion: Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.
Abstract
Background: Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria. Methods: The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe’s post-hoc test. The Kruskal–Wallis test was used to compare adhesion, followed by the Mann–Whitney test. Gene expression in the experimental and control samples was compared using the Student’s t-test. Results: Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups. Conclusion: Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.
참고문헌 (18)
- Kuramitsu HK, "Virulence properties of oral bacteria: impact of molecular biology", Curr Issues Mol Biol, 3, pp.35~36 (2001)
- Howard K. Kuramitsu, "Virulence Factors of Mutans Streptococci: Role of Molecular Genetics", Critical Reviews in Oral Biology & Medicine, 4(2), pp.159~176 (2016), SAGE Publications[DOI]
- Hye‐Young Shin, "Anti‐inflammatory Activity of Korean Folk Medicine Purple Bamboo Salt", Immunopharmacology and Immunotoxicology, 25(3), pp.377~384 (2003), Informa UK Limited[DOI]
- Chung SY, "Pharmacological evaluation of bamboo salt", Biomol Ther, 7, pp.178~184 (1999)
- 최충호, "죽염과 불소 혼합용액의 우치 초기인공우식법랑질 재광화 효과", 대한구강보건학회지, 36(1), pp.7~12 (2012), 대한예방치과·구강보건학회
- Fernanda C. Petersen, "Combined effects of NaF and SLS on acid- and polysaccharide-formation of biofilm and planktonic cells", Archives of Oral Biology, 51(8), pp.665~671 (2006), Elsevier BV[DOI]
- S. K. Filoche, "Antimicrobial effects of essential oils in combination with chlorhexidine digluconate", Oral Microbiology and Immunology, 20(4), pp.221~225 (2005), Wiley[DOI]
- K. Takarada, "A comparison of the antibacterial efficacies of essential oils against oral pathogens", Oral Microbiology and Immunology, 19(1), pp.61~64 (2004), Wiley[DOI]
- 오한나, "죽염이 Streptococcus mutans의 우식활성에 미치는 영향", 대한구강보건학회지, 40(4), pp.238~243 (2016), 대한예방치과·구강보건학회
- Park YN, "The effect of erythritol on gtf and ftf expression from Streptococcus mutans", doctoral, pp.2011
- R. Tarsi, "Inhibition of Streptococcus mutans Adsorption to Hydroxyapatite by Low-molecular-weight Chitosans", Journal of Dental Research, 76(2), pp.665~672 (2016), SAGE Publications[DOI]
- PC Baehni, "Anti-plaque agents in the prevention of biofilm-associated oral diseases", Oral Diseases, 9(s1), pp.23~29 (2003), Wiley[DOI]
- T. Ooshima, "Oolong Tea Polyphenols Inhibit Experimental Dental Caries in SPF Rats Infected with Mutatis Streptococci", Caries Research, 27(2), pp.124~129 (1993), S. Karger AG[DOI]
- Scheie AA, "Modes of action of currently known chemical anti-plaque agents other than chlorhexidine", J Dent Res, 68, pp.1609~1616 (1989)
- 김영희, "죽염의 제조과정에 따른 성분함량의 변화 및 타 염류와의 비교", 약 학 회 지, 47(3), pp.135~141 (2003), 대한약학회
- D.J. Bradshaw, "Effects of Glucose and Fluoride on Competition and Metabolism within in vitro Dental Bacterial Communities and Biofilms", Caries Research, 36(2), pp.81~86 (2002), S. Karger AG[DOI]
- H. K. Kuramitsu, "Interspecies Interactions within Oral Microbial Communities", Microbiology and Molecular Biology Reviews, 71(4), pp.653~670 (2007), American Society for Microbiology[DOI]
- Yamashita Y, "Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model", Infect Immun, 61, pp.3811~3817 (1993)