S. mutans에 대한 대나무 숯의 항균력에 관한 연구
Antibacterial effect of bamboo charcoal on Streptococcus mutans
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초록
Objectives : The purpose of the present study was to investigate the effect of bamboo charcoalon Streptococcus mutans which is one of the most important causative agents of dental caries. Methods : S. mutans was incubated with or without bamboo charcoal and then changes wereobserved in its cell viability and antibacterial effect. Oral epithelial cells viabillity(human gingivalfibroblast, HGF) was performed using MTT assay. Antibacterial effect was analyzed using a dilutionplating method and agar diffusion method. Results : Oral epithelial cells, human gingival fibroblast (HGF) showed a tendency to increasein bamboo charcoal treatment solution concentrations(0.5 , 1, 2 , 3 , 5, 10%). The bamboocharcoal had an antibacterial effect on S. mutans. Antibacterial effect of bamboo charcoal forthe bacterium was 58%. Charcoal concentration of 2% and 5% in the inhibition zone showeda minimal growth, but the concentration of 10% bamboo charcoal in inhibition zone revealeda conspicuous antibacterial activity. Conclusions : Overall results suggested that the bamboo charcoal proved to be bactericidaleffect on S. mutans.
Abstract
Objectives : The purpose of the present study was to investigate the effect of bamboo charcoalon Streptococcus mutans which is one of the most important causative agents of dental caries. Methods : S. mutans was incubated with or without bamboo charcoal and then changes wereobserved in its cell viability and antibacterial effect. Oral epithelial cells viabillity(human gingivalfibroblast, HGF) was performed using MTT assay. Antibacterial effect was analyzed using a dilutionplating method and agar diffusion method. Results : Oral epithelial cells, human gingival fibroblast (HGF) showed a tendency to increasein bamboo charcoal treatment solution concentrations(0.5 , 1, 2 , 3 , 5, 10%). The bamboocharcoal had an antibacterial effect on S. mutans. Antibacterial effect of bamboo charcoal forthe bacterium was 58%. Charcoal concentration of 2% and 5% in the inhibition zone showeda minimal growth, but the concentration of 10% bamboo charcoal in inhibition zone revealeda conspicuous antibacterial activity. Conclusions : Overall results suggested that the bamboo charcoal proved to be bactericidaleffect on S. mutans.
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