Neutral Electrolyzed Water for Prevention of Dental Caries
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Gargle solution has typically been used for the prevention of oral infectious disease such as dental caries and periodontitis. However, the use of most gargle solutions is controversial in application for children because some gargle solutions have harmful side effects. Electrolyzed water is generated by passed an electric current and has antimicrobial activity. The purpose of this study was to investigate and compare the efficacy of electrolyzed water in various conditions for eliminating cariogenic bacteria. Electrolyzed water was generated by a platinum electrode in the presence of sodium chloride at various concentrations. Streptococcus mutans and Streptococcus sobrinus were cultivated into a brain heart infusion broth. After harvesting planktonic bacteria, the pellets were treated with the electrolyzed water and commercial gargle solutions and plated on a mitis-salivarius agar plate. Also, the anti-biofilm activity of the electrolyzed water and commercial gargle solutions was investigated after biofilm formation of S. mutans and S. sobrinus. The bacteria in the biofilm were plated onto a mitis-salivarius agar plate. The plates were incubated, and the colony forming unit was measured. The electrolyzed water containing sodium chloride showed significant antibacterial activity against S. mutans and S. sobrinus as well as some gargle solutions. Furthermore, the electrolyzed water had more disruptive effect on the biofilm of S. mutans and S. sobrinus and killed more bacteria in the biofilm than commercial gargle solutions. The results demonstrate that electrolyzed water may be a useful gargle solution for prevention of dental caries.
Abstract
Gargle solution has typically been used for the prevention of oral infectious disease such as dental caries and periodontitis. However, the use of most gargle solutions is controversial in application for children because some gargle solutions have harmful side effects. Electrolyzed water is generated by passed an electric current and has antimicrobial activity. The purpose of this study was to investigate and compare the efficacy of electrolyzed water in various conditions for eliminating cariogenic bacteria. Electrolyzed water was generated by a platinum electrode in the presence of sodium chloride at various concentrations. Streptococcus mutans and Streptococcus sobrinus were cultivated into a brain heart infusion broth. After harvesting planktonic bacteria, the pellets were treated with the electrolyzed water and commercial gargle solutions and plated on a mitis-salivarius agar plate. Also, the anti-biofilm activity of the electrolyzed water and commercial gargle solutions was investigated after biofilm formation of S. mutans and S. sobrinus. The bacteria in the biofilm were plated onto a mitis-salivarius agar plate. The plates were incubated, and the colony forming unit was measured. The electrolyzed water containing sodium chloride showed significant antibacterial activity against S. mutans and S. sobrinus as well as some gargle solutions. Furthermore, the electrolyzed water had more disruptive effect on the biofilm of S. mutans and S. sobrinus and killed more bacteria in the biofilm than commercial gargle solutions. The results demonstrate that electrolyzed water may be a useful gargle solution for prevention of dental caries.
참고문헌 (27)
- Loesche WJ, "Role of Streptococcus mutans in human dental decay", Microbiol Rev, 50, pp.353~380 (1986)
- Pandit S, "Effects of Withania somnifera on the growth and virulence properties of Streptococcus mutans and Streptococcus sobrinus at sub-MIC levels", Anaerobe, 19, pp.1~8 (2013)
- Lee SH, "The cariogenic characters of xylitol-resistant and xylitol-sensitive Streptococcus mutans in biofilm formation with salivary bacteria", Arch Oral Biol, 57, pp.697~703 (2012)
- Neves PA, "Breastfeeding, Dental Biofilm Acidogenicity, and Early Childhood Caries", Caries Res, 50, pp.319~324 (2016)
- Savas S, "Effects of different antibacterial agents on enamel in a biofilm caries model", J Oral Sci, 57, pp.367~372 (2015)
- Monchois V, "Glucansucrases: mechanism of action and structurefunction relationships", FEMS Microbiol Rev, 23, pp.131~151 (1999)
- Baker K, "Mouthrinses in the prevention and treatment of periodontal disease", Curr Opin Periodontol, 1, pp.89~96 (1993)
- Rapani E, "Mouthrinses in the prevention of dental caries", Riv Odontostomatol Implantoprotesi, 92, pp.89~90 (1985)
- Reidy JT, "A review of the role of alcohol in the pathogenesis of oral cancer and the link between alcohol-containing mouthrinses and oral cancer", J Ir Dent Assoc, 57, pp.200~202 (2011)
- McCullough MJ, "The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes", Aust Dent J, 53, pp.302~305 (2008)
- Chevalier M, "Antiseptic mouthwashes could worsen xerostomia in patients taking polypharmacy", Acta Odontol Scand, 73, pp.267~273 (2015)
- Hricova D, "Electrolyzed water and its application in the food industry", J Food Prot, 71, pp.1934~1947 (2008)
- Prado-Silva L, "Meta-analysis of the effects of sanitizing treatments on Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes inactivation in fresh produce", Appl Environ Microbiol, 81, pp.8008~8021 (2015)
- Boyce JM, "Modern technologies for improving cleaning and disinfection of environmental surfaces in hospitals", Antimicrob Resist Infect Control, 5, pp.1~10 (2016)
- Nagamatsu Y, "Application of neutral electrolyzed water to disinfection of alginate impression", Dent Mater J, 35, pp.270~277 (2016)
- Horiba N, "Bactericidal effect of electrolyzed neutral water on bacteria isolated from infected root canals", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 87, pp.83~87 (1999)
- Kato S, "Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes", J Photochem Photobiol B, 106, pp.24~33 (2012)
- Ye J, "Inhibitory effect of electrolyzed reduced water on tumor angiogenesis", Biological & pharmaceutical bulletin, 31, pp.19~26 (2008)
- 이성훈, "Antibacterial Effect of Electrolyzed Water on Oral Bacteria", The Journal of Microbiology, 44(4), pp.417~422 (2006), 한국미생물학회
- Moberg L, "An improved N,N'-diethylp-phenylenediamine (DPD) method for the determination of free chlorine based on multiple wavelength detection", Analytica Chimica Acta, 407, pp.127~134 (2000)
- Lee SH, "A comparative study of the effect of probiotics on cariogenic biofilm model for preventing dental caries", Arch Microbiol, 196, pp.601~609 (2014)
- Beighton, D, "Associations between salivary levels of Streptococcus mutans, Streptococcus sobrinus, lactobacilli, and caries experience in Kenyan adolescents", J Dent Res, 68, pp.1242~1246 (1989)
- Miranzadeh S, "A New mouthwash for Chemotherapy Induced Stomatitis", Nurs Midwifery Stud, 3, pp.e20249~ (2014)
- Potting CM, "The effectiveness of commonly used mouthwashes for the prevention of chemotherapyinduced oral mucositis: a systematic review", Eur J Cancer Care, 15, pp.431~439 (2006)
- Cho M, "Mechanisms of Escherichia coli inactivation by several disinfectants", Water Res, 44, pp.3410~3418 (2010)
- Choi EJ, "Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries", Int J Paediatr Dent, 19, pp.141~147 (2009)
- Kreth J, "Role of sucrose in the fitness of Streptococcus mutans", Oral Microbiol Immunol, 23, pp.213~219 (2008)