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膈下逐瘀湯이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 肝癌 形成과 肝 損傷에 미치는 영향

The Effect of the Keughachukeo-tang Extract on the Hepatocellular Carcinogenesis and Acute Liver Damage Induced by Diethylnitrosamine and CCl4 in Rats

문구|이종덕|권소연|원진희|성기문|김소연|제준태|허래경
대한약침학회 (Korean Pharmacopuncture Institute)|2009.12|Journal of Pharmacopuncture|제12권 제4호|pp. 63-76

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참고문헌

키워드

p53CCl4DENAHepatocellular CarcinogenesisKeughachukeo-tang
주제분류한의학

초록

This study was performed to observe the effect of Keughachukeo-tang(KH) extract on the hepatocellular carcinogenesis and acute liver damage induced by Diethylnitrosamine(DENA) and CCl4 in Rats. Experimental groups were divided into four; normal group(Nor), acute liver damage and hepatocellular cancer inducing control group(Con), KH extract 350㎎/㎏/day(KHA), and 700㎎/㎏/day(KHB) administered groups to Con. The results obtained are as follows: The body weight increased in KHA and KHB than Con from 7th week to the 8th week. The activities of Alanine aminotransferase(ALT) were the most increased in the Con among experimental group. The activities of aspartate aminotransferase(AST), alkaline phosphatase(ALP), and lactacte dehydrogenase(LDH) were significantly decreased(p<0.05) in the KHA and KHB compared with Con. Alpha fetoprotein(AFP) were the most increased in the Con among experimental groups. The activities of superoxide dismutase(SOD) were the most increased in the Con among experimental groups. The activities of catalase were significantly increased(p<0.05) in the KHA and KHB compared with Con. The results of light microscopical observation, a number of hepatocytes were damaged in the Con compared with Nor and KH extract administerd groups. The number of hepatic p53 positive cells was reduced in the KH extract administered groups compared with Con. These results suggest that administration of KH extract suppress or retard on the Hepatocellular Carcinogenesis and acute liver damage induced by DENA and CCl4 in Rats.

Abstract

This study was performed to observe the effect of Keughachukeo-tang(KH) extract on the hepatocellular carcinogenesis and acute liver damage induced by Diethylnitrosamine(DENA) and CCl4 in Rats. Experimental groups were divided into four; normal group(Nor), acute liver damage and hepatocellular cancer inducing control group(Con), KH extract 350㎎/㎏/day(KHA), and 700㎎/㎏/day(KHB) administered groups to Con. The results obtained are as follows: The body weight increased in KHA and KHB than Con from 7th week to the 8th week. The activities of Alanine aminotransferase(ALT) were the most increased in the Con among experimental group. The activities of aspartate aminotransferase(AST), alkaline phosphatase(ALP), and lactacte dehydrogenase(LDH) were significantly decreased(p<0.05) in the KHA and KHB compared with Con. Alpha fetoprotein(AFP) were the most increased in the Con among experimental groups. The activities of superoxide dismutase(SOD) were the most increased in the Con among experimental groups. The activities of catalase were significantly increased(p<0.05) in the KHA and KHB compared with Con. The results of light microscopical observation, a number of hepatocytes were damaged in the Con compared with Nor and KH extract administerd groups. The number of hepatic p53 positive cells was reduced in the KH extract administered groups compared with Con. These results suggest that administration of KH extract suppress or retard on the Hepatocellular Carcinogenesis and acute liver damage induced by DENA and CCl4 in Rats.

참고문헌 (39)

  1. Devita VT, "Cancer. Principles and Practice of Oncology", pp.7~ (2004), Lippincott Williams & Wilkins
  2. 의학교육연구원, "가정의학", pp.266~ (1995), 서울대학교 출판부
  3. 통계청 편집부, "사망원인통계연보 전국편(`2006)" (2007)
  4. No HJ, "Experimental Syudies on the Antitumor Effects of Sojeokbojungwhan Extract", J of Korean Oriental Oncology, 2(1), pp.43~56 (1996)
  5. Jeong HW, "Experimental Study of NaetakKanghwal-Tang against tumor and immunologic control function", Korean J of oriental medical pathology, 11(2), pp.81~91 (1997)
  6. Yu DH, "Effects of Sa-Mul-Tang on Immunocytes of L1210 Celltransplanted or Antitumor Drugs-administered Mice", Korean J of Pharmacognosy, 29(2), pp.110~119 (1998)
  7. Baek DG, "Anticancer Effect of Paljinhangahm- dan on Human Acute Myeloid Leukemia", J of Wonkwang Uni. (2002)
  8. Baek EG, "Anticancer Effect of Combination with Paljinhangaham-dan and adriamycin on HepG2 Human Malignant Hepatoma Cell Line", J of Wonkwang Uni. (2003)
  9. 王淸任, "醫林改錯", pp.66~68 (1992), 一中社
  10. 余桂淸, "􆨤代中醫腫類案論選粹", pp.1~49 (1988), 北京出版社
  11. Lee KH, "A Study on the Effect of Keughachukeo-tang on Thrombosis and elevated Blood Viscosity", J of Kyunghee Uni., pp.15~22 (1992)
  12. Reitman S, "A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases", Am J Clin Pathol., 28, pp.56~63 (1987)
  13. Lee HS, "Specificities of serum alpha-fetoprotein in HBsAg+ and HBsAg- patients in the diagnosis of hepatocellular carcinoma", Hepatology, 14, pp.68~72 (1991)
  14. Sato Y, "Early recognition of hepatocellular carcinoma based on altered profiles of alpha-fetoprotein", N Engl J Med., 328, pp.1802~1806 (1993)
  15. Beers RF Jr, "A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase", J Biol Chem., 195(1), pp.133~140 (1992)
  16. 이문호, "내과학", pp.2458~2482 (1970), 금강출판사
  17. 서정선, "20세기말의 의 생물학의 새로운 비전 apoptosis 몸의 효율적 생존을 위한 세포의 능동적 사망기전", pp.30~32 (1998), 의학연구의 최신동향
  18. 王氷, "黃帝內經", pp.91~ (1977), 高文社
  19. 上海中醫學院, "實用中醫內科學", pp.621~635 (1986), 上海科學技術出版社
  20. 신민교, "臨床本草學", pp.172~175 (2000), 남산당
  21. 李時珍, "本草綱目", pp.401~ (1983), 高文社
  22. 許浚, "東醫寶鑑", pp.731~ (1986), 南山堂
  23. Kim HS, "The Effect of Nutritional Support with Nucare^R in Cancer Patients Receiving Chemotherapy", Chungnam medical J., 24(2), pp.181~189 (1997)
  24. 이귀녕, "임상병리파일", pp.334~337 (2003), 의학문화사
  25. Takeda, Y, "The biochemistry of animal cells the effect of corticosteroids on leakage of enzyme from dispersed rat liver cell", J Biol Chem, 239, pp.3590~3596 (1964)
  26. Brucker, JV, "Oral toxicity of carbon tetrachloride Acute, subacute, and subchronic studies in rats", Fund Appl Toxicol, pp.616~634 (1986)
  27. Dinman, BD, "CCl4 Toxicity III", Hepatostructural and enzyme change Arch Environ Health, 7, pp.630~646 (1963)
  28. 대한임상병리학회, "임상병리학", pp.67~76 (1996), 고려의학
  29. Henry RJ, "Clinical chemistry principles and techniques", pp.2~ (1974), Harper and Row
  30. Lee JH, "Relationship Between the Size of Hetatocellular Carcinoma and Serum Alpha-Fetoprotein Level and Ultrasonographic Echogenicity", Korean J Gastroenterol., 20(3), pp.679~684 (1988)
  31. Fieg D.I, "L.A. Reactive oxygen species in tumorigenesis", Cancer Res., pp.1890~1894 (1994)
  32. Diguiseppi. J, "Oxygen toxicity in streptococcus sangius", J. biol. Chem., 257, pp.4046~4051 (1982)
  33. Bellisola G, "Catalase activity in human hepatocellular carcinoma(HCC)", Italy. Clin Biochem., 20(6), pp.415~417 (1987)
  34. Strobel P, "Xenomorphic hepatocellular precusor and neoplastic progression of tigroid cell foci induced in rats with low doses of N-nitrosomorpholine", Carcinogenesis, 19, pp.2069~2080 (1998)
  35. Steinberg P, "Eigenbrodt. Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats", Virchows Arch, 434, pp.213~220 (1999)
  36. Yu E, "Progressive changes of the nuclear matrix protein composition in diethylnitrosamine induced rat hepatocarcinogenesis", Cancer Lett, 138, pp.151~157 (1999)
  37. Pruschy M, "Key targets for the execution of radiation induced tumor cell apoptosis : the role of p53 and caspases", Int. J. Radiat. Oncol. Biol. Phys., 49(2), pp.561~567 (2001)
  38. Jessup JM, "Prognostic markers in rectal carcinoma", Semin Surg Oncol., 15, pp.131~140 (1998)
  39. Sturm I, "Impaired BAX proteins expression in breast cancer:mutational analysis of the Bax and the p53 gene", Int J Cancer, 87, pp.517~521 (2000)

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