인진호탕 추출액의 투여가 흰쥐의 간암 발생에 미치는 효과
The Effect of the Injinhotang Extract on Hepatocarcinogenesis in Rats
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본 실험은 N-nitrosodiethylamine (NDEA)와 사염화탄소(CCl4) 로 유발된 흰쥐의 간암에 대한 인진호탕의 효과를 알아보고자 하였다. 먼저 실험군 설정은 세 군으로 분류하였다. 정상군(Nor) 에는 일반적인 식이를 공급하였고, 대조군(Con)에는 NDEA(200 mg/kgb.w., i.p.)와 CCl4를 투여하였고, 인진호탕 추출액 투여군 (IJH)에는 인진호탕 추출액(260 mg/kg/day)을 8주 동안 투여하 였다. Superoxide dismutase (SOD)와 catalase와 같은 항산화계 효소를 측정한 결과, SOD의 활성은 Con군에서 증가하였으나, catalase의 활성은 Con군에서 감소하였다. 면역조직화학적 관찰 에서, 인진호탕 추출액의 투여로 인해 Con군에서보다 p53 면역 반응율을 감소시켰다. 전자현미경 관찰의 경우, 간암 세포들은 Con군에서 과립세포질세망의 팽대와 무과립세포질세망의 증가 가 뚜렷하게 관찰되었다. 이상의 결과로 보아 인진호탕 추출액 투여가 NDEA와 사염화탄소로 유도된 흰쥐의 간암을 억제 또는 지연하는 것으로 사료된다.
Abstract
In order to examine the effect of Injinhotang extract on the liver cancer induced by N-nitrosodiethylamine (NDEA) and carbon tetrachloride (CCl4) in Rats. The animals were divided into three groups. The normal (Nor) group were fed basal diet. Control (Con) group were administered with NDEA (200 mg/kgb.w., i.p.) and CCl4. Injinhotang extract (IJH) group treated with Injinhotang extract (260 mg/kg/day) for 8 weeks after NDEA+CCl4. Enzymic antioxidants, such as superoxide dismutase (SOD) and catalase levels were determined in all the groups of animals. The activities of SOD were significantly increased in the Con, but the activities of catalase were decreased in the Con, but the anti-oxidative enzyme activities of superoxide dismutase and catalase were increased in the IJH. In the immunohistochemistry observation, treatment of Injinhotang extract reduced the rates of p53 immunoreactivity. According to the electron microscopical observation, in the liver cancer cells were increased the smooth endoplasmic reticulum and dilated the rough endoplasmic reticulum in the Con compared with IJH. These results suggest that administration of Injinhotang extract suppress or retard NDEA and CCl4-induced liver cancer.
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