女貞子, 旱蓮草 및 二至丸의 항산화활성 및 혈압에 미치는 영향에 관한 연구
A study on effects of Ligustri Lucidi Fructus, Ecliptae Herba and Yijihwan on antioxidant activity and blood pressure
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Purpose: Ligustri Lucidi Fructus and Ecliptae Herba has long been used for clinical therapy associated especially with menopausal symptoms in Korea. To provide a scientific rationale for such use, we have investigated the antioxidant and vasorelaxant effects of Ligustri Lucidi Fructus, Ecliptae Herba and its mixture. Methods: The antioxidant activity of the extracts from Ligustri Lucidi Fructus, Ecliptae Herba and its mixture were evaluated and compared with that of BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene), vitamin C and vitamin E, using the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. Results: Antioxidant activity of all extracts using the DPPH radical scavenging method decreased in the order vitamin C>BHA>vitamin E>Ligustri Lucidi Fructus>Ligustri Lucidi Fructus:Ecliptae Herba(1:1)>Ecliptae Herba>BHT. The vasorelaxant effects of extracts were investigated on the vasomotor tone of the rat thoracic aorta in an organ bath. All of the extracts elicited a long-term relaxing response in the endothelium-intact as well as endothelium-denuded rat aorta contracted with norepinephrine. This relaxant effect was abolished by precontraction with 72 mM KCl. Thus, it is suggested that the mechanism of vasorelaxant effect of extracts not involve voltage-operated Ca2+ channel blocking but receptor-mediated route. Conclusion: These antioxidant and vasorelaxant effecs of the extracts may contribute to the beneficial effects in postmenopausal women.
Abstract
Purpose: Ligustri Lucidi Fructus and Ecliptae Herba has long been used for clinical therapy associated especially with menopausal symptoms in Korea. To provide a scientific rationale for such use, we have investigated the antioxidant and vasorelaxant effects of Ligustri Lucidi Fructus, Ecliptae Herba and its mixture. Methods: The antioxidant activity of the extracts from Ligustri Lucidi Fructus, Ecliptae Herba and its mixture were evaluated and compared with that of BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene), vitamin C and vitamin E, using the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging method. Results: Antioxidant activity of all extracts using the DPPH radical scavenging method decreased in the order vitamin C>BHA>vitamin E>Ligustri Lucidi Fructus>Ligustri Lucidi Fructus:Ecliptae Herba(1:1)>Ecliptae Herba>BHT. The vasorelaxant effects of extracts were investigated on the vasomotor tone of the rat thoracic aorta in an organ bath. All of the extracts elicited a long-term relaxing response in the endothelium-intact as well as endothelium-denuded rat aorta contracted with norepinephrine. This relaxant effect was abolished by precontraction with 72 mM KCl. Thus, it is suggested that the mechanism of vasorelaxant effect of extracts not involve voltage-operated Ca2+ channel blocking but receptor-mediated route. Conclusion: These antioxidant and vasorelaxant effecs of the extracts may contribute to the beneficial effects in postmenopausal women.
참고문헌 (23)
- "The influence of menopause on blood pressure", pp.427~433 (1989)
- "Coronary heart disease risk factors in relation to the menopause", pp.889~896 (1992)
- "Estrogen replacement in menopause", 81, pp.3829~3838 (1996)
- "Expression of an estrogen receptor by human coronary artery and umbilical vein endothelial cells", 94, pp.1402~1407 (1996)
- "Protective effect of 17-beta-estradiol against the cytotoxicity of minimally oxidized LDL to cultured bovine aortic endothelial cells", pp.207~217 (1993)
- "Risk and Benefits of Estrogen plus Progestin in healthy postmenopausal women", 288(3), pp.321~333 (2002)
- "Estrogen plus Progestin and the Risk of Coronary Heart Diseas", 349(6), pp.523~534 (2003)
- "Use of Alternative Therapies for Menopause Symptoms: Results of a population-Based Survey", 100(1), pp.18~25 (2002)
- "Use of a free radical method to evaluate antioxidant activity", pp.25~30 (1985)
- "Spectrophotometric assay and properties of the angiotensin converting enzyme of rabbit lung", pp.1637~1648 (1970)
- "Evaluation of extracts from gevunia avellana hulls as antioxidants", 48, pp.3890~3897 (2000)
- "Studies on the antioxidant activity of Indian laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp", 79, pp.61~67 (2002)
- "Comparison of the antioxidant activity of rooibos tea (Aspalathus linearis) with green, oolong and black tea", 60, pp.73~77 (1997)
- "Secoiridoid constituents from the fruits of Ligusticum lucidum", 56, pp.327~330 (2001)
- "Studies on the antilipid peroxidation of nine sorts of Chinese herbal medicines with the function of protecting liver", 20, pp.624~626 (1997)
- "Textbook of medical physiology", the auto(mic nervous system), pp.671~673 (1991)
- "Determination of rutin and quercetin in plants by capillary electrophoresis with electrochemcal detection", pp.69~76
- "Mechanism of vascular smooth muscle relaxation by estrogen in depolarized rat and mouse aorta. Role of nuclear estrogen receptor and Ca2+ uptake", 81, pp.242~248 (1997)
- "中藥大辭典(6)", pp.3783~3786 (1998), 서울;도서출판 정담
- "오배자의 항산화 및 자유라디칼 소거 효과", 6(3), pp.181~187 (1998)
- "韓醫婦人科學(上)", pp.242~243 (2002), 서울;도서출판 정담
- "된장으로부터 Angiotensin Converting Enzyme 활성 저해물질의 정제", pp.441~446 (1994)
- "심혈관질환의 분자세포생물학", pp.76~83 (1998), 서울;고려의학