호장근 물추출물의 내피세포 의존성 혈관이완효과
Endothelium Dependent Vasorelaxant Effect of Aqueous Extract of Polygoni Cuspidatae Radix on Arterial Contraction in Rabbit
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This study was undertaken to define the effect of Polygoni cuspidatae Radix on contracted rabbit common carotid artery and its mechanism. In order to investigate the effect of Polygoni cuspidatae Radix on rabbit's contracted vascular ring detached from common carotid artery, vascular ring with intact or damaged endothelium was used for the experiment using organ bath. To analyze the mechanism of Polygoni cuspidatae Radix-induced relaxation, Polygoni cuspidatae Radix extract was infused into contracted vascular ring which had been pretreated by Nω-nitro-L-arginine(L-NNA), Methylene blue(MB), and Ca2+ was infused into contracted vascular ring induced by NE or KCl after treatment of Polygoni cuspidatae Radix extract in Ca2+-free solution. The results were as follows : Polygini cuspidatae Radix had an effective relaxation to the contracted vascular ring by NE in 0.1 mg/ml and 0.3 mg/ml level. Polygini cuspidatae Radix had an effective relaxation to the intact endothelium vascular ring, but when endothelium was removed, vascular ring did not relax. Polygini cuspidatae Radix-induced relaxation was inhibited by the pretreatment of L-NNA and MB. Pretreatment of Polygini cuspidatae Radix extract inhibit the contraction by influx of extra-Ca2+ in contracted vascular ring induced by NE or KCl in Ca2+-free solution. As mentioned above, we suggest that Polygini cuspidatae Radix relaxes vascular ring through suppress influx of extra-cellular Ca2+ by the action of nitric oxide from endothelium.
Abstract
This study was undertaken to define the effect of Polygoni cuspidatae Radix on contracted rabbit common carotid artery and its mechanism. In order to investigate the effect of Polygoni cuspidatae Radix on rabbit's contracted vascular ring detached from common carotid artery, vascular ring with intact or damaged endothelium was used for the experiment using organ bath. To analyze the mechanism of Polygoni cuspidatae Radix-induced relaxation, Polygoni cuspidatae Radix extract was infused into contracted vascular ring which had been pretreated by Nω-nitro-L-arginine(L-NNA), Methylene blue(MB), and Ca2+ was infused into contracted vascular ring induced by NE or KCl after treatment of Polygoni cuspidatae Radix extract in Ca2+-free solution. The results were as follows : Polygini cuspidatae Radix had an effective relaxation to the contracted vascular ring by NE in 0.1 mg/ml and 0.3 mg/ml level. Polygini cuspidatae Radix had an effective relaxation to the intact endothelium vascular ring, but when endothelium was removed, vascular ring did not relax. Polygini cuspidatae Radix-induced relaxation was inhibited by the pretreatment of L-NNA and MB. Pretreatment of Polygini cuspidatae Radix extract inhibit the contraction by influx of extra-Ca2+ in contracted vascular ring induced by NE or KCl in Ca2+-free solution. As mentioned above, we suggest that Polygini cuspidatae Radix relaxes vascular ring through suppress influx of extra-cellular Ca2+ by the action of nitric oxide from endothelium.
참고문헌 (32)
- 全國韓醫科大學 本草學敎授, "本草學", pp.420~421 (1998), 永林社
- 신민교, "臨床本草學", pp.476~477 (1986), 南山堂
- 上海中醫學院.中草藥學.上海, "商務印書館香港分館", pp.402~403 (1983), 商務印書館香港分館
- 新文豐出版公司, "中藥大辭典 第二冊", pp.1107~1109 (1982), 新文豐出版公司
- 許浚, "東醫寶鑑", pp.1962~ (1999), 법인문화사
- 두호경, "東醫腎系學(上)", pp.85~132 (1995), 동양의학연구원
- 王溶生, "中藥藥理與應用", pp.652~658 (1983), 人民衛生出版社
- 최병진, "韓藥의 藥理", pp.117~339 (2000), 고려의학
- 이태규, "虎杖根으로부터 분리된 emodin의 혈관신생 억제 활성", 한국농화학회지, 46(1), pp.50~54 (2003)
- 김신규, "호장근의 항세균효과 및 항부착효과", 대한구강보건학회지, 29(1), pp.80~90 (2005), 대한구강보건학회
- 김동일, "Gardnerella vaginalis 균의 생육을 억제하는 한약재 탐색에 관한 연구", 대한한방부인과학회지, 10(2), pp.97~115 (1997)
- 정운석, "百日咳 誘發菌 Bordetella pertussis에 대한 虎杖根의 生育 抑制 效果", 대한한방내과학회지, 26(3), pp.543~550 (2005), 대한한방내과학회
- 전국 한의과대학 심계내과학교실, "심계내과학", pp.189~196 (1999), 서원당
- 김종설, "고혈압의 이해와 치료", pp.10~13 (2002), 고려의학
- 임동윤, "리핀코트의 그림으로 보는 약리학", pp.187~189 (2002), 신일상사
- 조려화, "선학초 부탄올 추출물의 혈관 이완 효과의 기전에 대한 연구", 생약학회지, 37(2), pp.67~73 (2006), 한국생약학회
- 배명효, "山査의 내피세포 의존성 혈관이완효과 기전에 대한 연구", 大韓本草學會誌, 18(3), pp.169~180 (2003)
- 신형섭, "烏藥順氣散이 고혈압과 동맥혈관에 미치는 영향", 한국한의학연구원 논문집, 10(2), pp.79~92 (2004)
- 임준규, "동서의학협진과 임상실제", pp.47~67 (1995), 한성사
- 동서의학융합연구회편, "임상동서의학", pp.202~205 (1997), 영림사
- Johns, R.A., "Endothelium-derived relaxing factor;Basic review and clinical implications", Cardiothorac. Vasc. Anesth, 5(1), pp.69~79 (1991)
- Palmer, R.M.J., "Nitric oxide release accounts for the biological activity of endothelium -derived relaxing factor", Nature, 327, pp.524~526 (1987)
- Palmer, R.M., "L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation, Biochem", Biophys. Res. Commun, 153(3), pp.1251~1256 (1988)
- Moore, P.K., "L-NG-nitro arginine(L-NOARG), a novel, L-arginine-reversible inhibitor of endothelium-dependent vasodilatation in vitro", Br. J. Pharmacol, 99, pp.408~412 (1990)
- Nandor, M., "Endothelial cGMP does not regulate basal release of endothelium-derived relaxing factor in culture", Am. J. Physiol, 263, pp.113~121 (1992)
- Deth, R., "van, Relative contributions of Ca2+ influx and cellular Ca2+ release during drug induced activation of the rabbit aorta", Pflügers Arch, 348(1), pp.13~22 (1974)
- Fabiato, A., "Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, drug, cat, rabbit, rat, and frog hearts and from fetal and new-born rat ventricles", pp.491~521 (1978), Annuals New York Academy of Sciences
- Cynthia Cauvin, "Different Ca2+ channels along the arterial tree", Journal of Cardio vascular Pharmacology, 7(4), pp.4~10 (1985)
- 성호경, "생리학", pp.103~135 (1997), 의학문화사
- 안재호, "쥐 대동맥 혈관 내피세포에서 세포 외 K 에 의한 혈관 수축성 조절 기전", The Korean Journal of Thoracic and Cardiovascular Surgery, 36(12), pp.887~893 (2003), 대한흉부외과학회
- 서석효, "Effects of extracellular Ca2+ and Ca2+-antagonists on endothelium-dependent relaxation in rabbit aorta", 대한생리학회지, 24(1), pp.91~102 (1991)
- J.M. Farley., "Role of depolarization in acetylcholine-induced contractions of dog trachealis muscle", The Journal of Pharmacology and Experimental Therapeutics, 201, pp.199~205 (1997)