Aristolochia ringens extract ameliorates oxidative stress and dyslipidaemia associated with streptozotocin-induced hyperglycaemia in rats
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The study was designed to assess antioxidant and antidyslipidaemic effects of terpenoid-rich extract from the root of Aristolochia ringens V. Hyperglycemia-induced oxidative stress and dyslipidemia were established in rats by single intraperitoneal administration of 65 mg/kg bw streptozotocin. Based on therapeutic dose determined in previous study, streptozotocin-induced rats were orally administered with 75 and 150 mg/Kg bw of A. ringens extract for 14 days. Total protein, serum lipid profiles and biomarkers of oxidative stress in liver and kidney of the experimental rats were determined. Atherogenic and cardiovascular disease risk indices were computed. Streptozotocin-induced hyperglycaemia significantly (p < 0.05) decreased activities of superoxide dismutase, catalase and glutathione transferase as well as the amount of reduced glutathione in both tissues indicating oxidative stress induced kidney and liver injury due to glucotoxicity. In comparison to non-treated hyperglycaemic rats, activities of the antioxidant enzymes and concentration of glutathione-H were significantly (p < 0.0001) increased, whereas malondialdehyde was reduced in the tissues of rats treated with both 75 and 150 mg/Kg bw of the extract. The extract also caused significant (p < 0.001) reduction in elevated levels of total cholesterol, triglycerides and low density lipoprotein-cholesterol levels, whereas concentration of the attenuated high density lipoprotein-cholesterol was increased in serum of the treated rats. Reduced atherogenic and cardiac risk indices were projected for the A. ringens extract-treated groups. Results from this study showed that extract from A. ringens root was rich in terpenoids and may reduce risks of complications associated with hyperglycemia-induced oxidative stress and dyslipidemia.
Abstract
The study was designed to assess antioxidant and antidyslipidaemic effects of terpenoid-rich extract from the root of Aristolochia ringens V. Hyperglycemia-induced oxidative stress and dyslipidemia were established in rats by single intraperitoneal administration of 65 mg/kg bw streptozotocin. Based on therapeutic dose determined in previous study, streptozotocin-induced rats were orally administered with 75 and 150 mg/Kg bw of A. ringens extract for 14 days. Total protein, serum lipid profiles and biomarkers of oxidative stress in liver and kidney of the experimental rats were determined. Atherogenic and cardiovascular disease risk indices were computed. Streptozotocin-induced hyperglycaemia significantly (p < 0.05) decreased activities of superoxide dismutase, catalase and glutathione transferase as well as the amount of reduced glutathione in both tissues indicating oxidative stress induced kidney and liver injury due to glucotoxicity. In comparison to non-treated hyperglycaemic rats, activities of the antioxidant enzymes and concentration of glutathione-H were significantly (p < 0.0001) increased, whereas malondialdehyde was reduced in the tissues of rats treated with both 75 and 150 mg/Kg bw of the extract. The extract also caused significant (p < 0.001) reduction in elevated levels of total cholesterol, triglycerides and low density lipoprotein-cholesterol levels, whereas concentration of the attenuated high density lipoprotein-cholesterol was increased in serum of the treated rats. Reduced atherogenic and cardiac risk indices were projected for the A. ringens extract-treated groups. Results from this study showed that extract from A. ringens root was rich in terpenoids and may reduce risks of complications associated with hyperglycemia-induced oxidative stress and dyslipidemia.
참고문헌 (38)
- Abdulrazaq NB, "Beneficial effects of ginger (Zingiber officinale) on carbohydrate metabolism in streptozotocin-induced diabetic rats", British Journal of Nutrition, 108(7), pp.1194~1201 (2012)[DOI]
- Adeyemi O, "The antidiarrhoeal activity of the aqueous root extract of Aristolochia ringens (Vahl.) Aristolochiaceae", Nigerian quarterly journal of hospital medicine, 22(1), pp.29~33 (2012)
- Aigbe FR., "Alterations of haemodynamic parameters in spontaneously hypertensive rats by Aristolochia ringens Vahl. (Aristolochiaceae)", Journal of Traditional and Complementary Medicine, 8(1), pp.72~80 (2018)[DOI]
- Akindele AJ, "Anticancer activity of Aristolochia ringens Vahl. (Aristolochiaceae)", Journal of Traditional and Complementary Medicine, 5(1), pp.35~41 (2015)[DOI]
- Almuaigel MF, "Hypoglycemic and hypolipidemic effects of aqueous extract of phaseolus vulgaris pods in streptozotocin-diabetic rats", Biomedicine & Pharmacotherapy, 94, pp.742~746 (2017)[DOI]
- Armstrong D, "Lipid peroxidation and retinopathy in streptozotocin-induced diabetes", Free Radical Biology and Medicine, 11(4), pp.433~436 (1991)[DOI]
- Bandyopadhyay U, "Reactive oxygen species: Oxidative damage and pathogenesis", Current science, 77(5), pp.658~666 (1999)
- Draper HH, "A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials", Free Radical Biology and Medicine, 15(4), pp.353~363 (1993)[DOI]
- Edelstein D, "Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage", Nature, 404(6779), pp.787~790 (2000)[DOI]
- Ellman GL, "Tissue sulfhydryl groups", Archives of biochemistry and biophysics, 82(1), pp.70~77 (1959)[DOI]
- Friedewald WT, "Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge", Clinical chemistry, 18(6), pp.499~502 (1972)
- Grassmann J, "Terpenoids as plant antioxidants", Vitamins & Hormones, 72, pp.505~535 (2005)
- Grassmann J, "Plant's defence and its benefits for animals and medicine: role of phenolics and terpenoids in avoiding oxygen stress", Plant Physiology and Biochemistry, 40(6), pp.471~478 (2002)[DOI]
- Habig WH, "The identity of glutathione S-transferase B with ligandin, a major binding protein of liver", Proceedings of the National Academy of Sciences, 71(10), pp.3879~3882 (1974)[DOI]
- Huber H, "Flowering Plants.Dicotyledons. The Families and Genera of Vascular Plants, vol 2", pp.129~137 (1993), Springer
- Institute for Laboratory Animal Research(U.S.), "Guide for the Care and Use of Laboratory Animals" (2010), National Academies Press
- Kalinina E, "Role of glutathione, glutathione transferase, and glutaredoxin in regulation of redox-dependent processes", Biochemistry (Moscow), 79(13), pp.1562~1583 (2014)[DOI]
- Kostalova D, "Constituents of Aristolochia-clematitis L", Chemical Papers, 45(5), pp.713~716 (1991)
- Loumouamou AN, "Optimization of the extraction of the pmenthadienol isomers and aristolone contained in the essential oil from Elyonurus hensii using a 23 full factorial design", Food Science & Nutrition, 5(3), pp.784~792 (2017)[DOI]
- Lowry OH, "Protein measurement with the Folin phenol reagent", Journal of biological chemistry, 193(1), pp.265~275 (1951)
- Madhuri K, "Ameliorative effect of borneol, a natural bicyclic monoterpene against hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic Wistar rats", Biomedicine & Pharmacotherapy, 96, pp.336~347 (2017)[DOI]
- Marfella R, "Polyphenols, Oxidative Stress, and Vascular Damage in Diabetes Diabetes: oxidative stress and dietary antioxidants" (2014), Academic Press
- Marrelli M, "Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells", Food and Chemical Toxicology, 86, pp.16~24 (2015)[DOI]
- Misra HP, "The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase", Journal of Biological Chemistry, 247(10), pp.3170~3175 (1972)
- Nazaruk J, "The role of triterpenes in the management of diabetes mellitus and its complications", Phytochemistry Reviews, 14(4), pp.675~690 (2015)[DOI]
- Nishikawa T, "Antitrypanosomal activity of Aristolochia ringens against Trypanosoma congolense infection in mice", Journal of Pharmacognosy and Phytotherapy, 6(1), pp.1~3 (2014)[DOI]
- Rani MP, "HPTLC and reverse phase HPLC methods for the simultaneous quantification and in vitro screening of antioxidant potential of isolated sesquiterpenoids from the rhizomes of Cyperus rotundus", J Chromatogr B Analyt Technol Biomed Life Sci, 904, pp.22~28 (2012)[DOI]
- Ruth AF, "The aqueous root extract of Aristolochia ringens (Vahl.) Aristolochiaceae inhibits chemically-induced inflammation in rodents", Pak. J. Pharm. Sci, 27(6), pp.1885~1889 (2014)
- Saljoughian S, "The effects of food essential oils on cardiovascular diseases: A review", Critical Reviews in Food Science and Nutrition, 58(10), pp.1688~1705 (2018)[DOI]
- Silou T, "Extraction of Elionurus Hensii K. Schum Essential Oil in a Domestic Scale in the "Plateau Des Cataractes" (Congo-Brazzaville)", International Journal of Engineering Science Technologies, 2(1) (2017)
- Sindhu RK, "Dysregulation of hepatic superoxide dismutase, catalase and glutathione peroxidase in diabetes: response to insulin and antioxidant therapies", Clinical and experimental hypertension, 26(1), pp.43~53 (2004)[DOI]
- Sinha AK, "Colorimetric assay of catalase", Analytical biochemistry, 47(2), pp.389~394 (1972)[DOI]
- Sulyman A, "Antidiabetic potentials of ethanolic extract of Aristolochia ringens (Vahl.) roots", Journal of ethnopharmacology, 182, pp.122~128 (2016)[DOI]
- Sulyman AO, "Effect of Administration of Root Ethanolic Extract of Aristolochia Ringens on the Liver Functional Indices of Male Wistar Rats", Iranian Journal of Toxicology, 11(1), pp.55~58 (2017)[DOI]
- Szkudelski T, "The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas", Physiological research, 50(6), pp.537~546 (2001)
- Szkudelski T, "Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model", Experimental Biology and Medicine, 237(5), pp.481~490 (2012)[DOI]
- Szkudelski T, "Streptozotocin induces lipolysis in rat adipocytes in vitro", Physiological research, 51(3), pp.255~260 (2002)
- Tangvarasittichai S, "Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus", World journal of diabetes, 6(3), pp.456~ (2015)[DOI]
