Serum Biochemical, Histopathology and SEM Analyses of the Effects of the Indian Traditional Herb Wattakaka Volubilis Leaf Extract on Wistar Male Rats
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: The present study investigated the protective effect of Wattakaka (W.) volubilis leaf extract against streptozotocin (STZ)-induced diabetes in rats. Methods: Male Wistar rats were divided into five groups (with six rats in each group) and were fed ad libitum. The rats were fasted for sixteen hours before diabetes was induced by injecting a single dose of 90 mg/kg body weight of STZ in 0.9-percent normal saline through an intraperitoneal route. The five groups were as follows: Group 1: normal control (saline-treated), Group 2: untreated diabetic rats, Groups 3 and 4: diabetic rats treated orally with petroleum ether cold maceration extract (PEME) of W. volubilis (50 and 100 mg/kg body weight), and Group 5: diabetic rats treated orally with metformin (250 mg/kg body weight). All rats received treatment for 21 days. For the STZ-induced diabetic rats, the blood-glucose, α-amylase, total protein and alanine transaminase (ALT) levels were measured on days 7, 14 and 21 of the treatment with PEME of W. volubilis and the treatment with metformin. Histopathological changes in the liver were examined with hematoxylin-eosin staining. Morphological changes in the liver were also examined with glutaraldehyde fixation. Results: The treatments with PEME of W. volubilis and with metformin in experimental rats by oral injections for 21 days produced reductions in the levels of serum biochemical markers. Histopathology and scanning electron microscopy results showed that the administrations of PEME of W. volubilis and of metformin suppressed the generation of abnormal liver cells in the STZ-treated rats. Conclusion: These results suggest that both PEME of W. volubilis and metformin have a protective effect against STZ-induced diabetes.
Abstract
Objectives: The present study investigated the protective effect of Wattakaka (W.) volubilis leaf extract against streptozotocin (STZ)-induced diabetes in rats. Methods: Male Wistar rats were divided into five groups (with six rats in each group) and were fed ad libitum. The rats were fasted for sixteen hours before diabetes was induced by injecting a single dose of 90 mg/kg body weight of STZ in 0.9-percent normal saline through an intraperitoneal route. The five groups were as follows: Group 1: normal control (saline-treated), Group 2: untreated diabetic rats, Groups 3 and 4: diabetic rats treated orally with petroleum ether cold maceration extract (PEME) of W. volubilis (50 and 100 mg/kg body weight), and Group 5: diabetic rats treated orally with metformin (250 mg/kg body weight). All rats received treatment for 21 days. For the STZ-induced diabetic rats, the blood-glucose, α-amylase, total protein and alanine transaminase (ALT) levels were measured on days 7, 14 and 21 of the treatment with PEME of W. volubilis and the treatment with metformin. Histopathological changes in the liver were examined with hematoxylin-eosin staining. Morphological changes in the liver were also examined with glutaraldehyde fixation. Results: The treatments with PEME of W. volubilis and with metformin in experimental rats by oral injections for 21 days produced reductions in the levels of serum biochemical markers. Histopathology and scanning electron microscopy results showed that the administrations of PEME of W. volubilis and of metformin suppressed the generation of abnormal liver cells in the STZ-treated rats. Conclusion: These results suggest that both PEME of W. volubilis and metformin have a protective effect against STZ-induced diabetes.
참고문헌 (18)
- Andallu B., "Control of hyperglycemic and retardation of cataract by mulberry(Morusindica. L)leaves in streptozotocin diabetic rats", Ind J Exp Biol, 40(7), pp.791~795 (2002)
- Wild SG., "Global prevalence of diabetes : estimates for the year 2000 and projection for 2030", Diabetes Care, 27(5), pp.1047~1053 (2004)
- Kumar S., "Antidiabetic and free-radical scavenging potential of Euphorbia hirta flower extract", Indian J Pharm Sci, 72(4), pp.531~537 (2010)
- Kumar S, "D Evaluation of antidiabetic activity of Euphorbia hirta Linn. In streptozotocin induced diabetic mice", Indian J Nat Prod Resour, 1(2), pp.200~203 (2010)
- Eidi A., "Antidiabetic effects of sage(Salvia officinalis L. )leaves in normal and streptozotocin-induced diabetic rats", Diabetes Met SynClin Res Rev, 3(1), pp.40~44 (2009)
- Pandit R., "Antidiabetic effect of Ficus religiosa extract in streptozotocin-induced diabetic rats", J Ethnopharmacol, 128(2), pp.462~466 (2010)
- Tripathi AK., "Herbal antidiabetics : A review", Int J Res Pharm Sci, 2(1), pp.30~37 (2011)
- Ramachandran V., "Hypoglycemic, antioxidant and hypolipidemic activity of Asparagus racemosuson streptozotocin induced diabetes in rats", AdvApplSci Res, 2(3), pp.179~185 (2011)
- Panda N, "Pregnaneglycosides", Nat Prod Comm, pp.665~669 (2006)
- Ayyanar M., "Traditional herbal medicines used for the treatment of diabetes among two major tribal groups in South Tamil, Nadu, India", Ethnobotanical Leaflets, 12, pp.276~280 (2008)
- Kumarappan CT., "Modulatory effect of polyphenolic extracts of Ichnocarpus frutescens on oxidative stress in rats with experimentally induced diabetes", Indian J Med Res, 136(5), pp.815~821 (2012)
- Trinder P., "Determination of blood glucose using an oxidase-peroxidase system with a non-carginogenic chemogen", J Clin Pathol, 22(2), pp.158~161 (1969)
- Tarling CA., "The search for novel human pancreatic alpha-amylase inhibitors : high-throughput screening of terrestrial and marine natural product extracts", Chem Bio Chem, 9(3), pp.433~438 (2008)
- Vincent AM., "Oxidative stress in the pathogenesis of diabetic neuropathy", Endocr Rev, 25(4), pp.612~628 (2004)
- Mauer SM., "The kidney in diabetes", Am J Med, 70(3), pp.603~612 (1981)
- Almdal JP., "Strict insulin therapy normalizes organ nitrogen contents and capacity of urea nitrogen synthesis in experimental diabetes in rats", Diabetolgia, 31(2), pp.114~118 (1988)
- Agbaje EO., "Biochemical and toxicological studies of aqueous extract of syzigium aromaticum(L. )merr. & perry(myrtaceae)in rodent", Aft J Tradit Complement Alter Med, 6(3), pp.241~254 (2009)
- Davern TJ, "Sleisenger and Fordtran’s Gastrointestinal and liver disease: pathophysiology, diagnosis, management. 7th ed", pp.1227~1228 (2002), W B Saunders Co