Anti-Diabetic Effects of an Ethanol Extract of Cassia Abbreviata Stem Bark on Diabetic Rats and Possible Mechanism of Its Action - Anti-diabetic Properties of Cassia abbreviata -
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Objectives: This study aimed to evaluate the hypoglycemic effects of an ethanol extract of Cassia abbreviata (ECA) bark and the possible mechanisms of its action in diabetic albino rats. Methods: ECA was prepared by soaking the powdered plant material in 70% ethanol. It was filtered and made solvent-free by evaporation on a rotary evaporator. Type 2 diabetes was induced in albino rats by injecting 35 mg/ kg body weight (bw) of streptozotocin after having fed the rats a high-fat diet for 2 weeks. Diabetic rats were divided into ECA-150, ECA-300 and Metformin (MET)-180 groups, where the numbers are the doses in mg.kg.bw administered to the groups. Normal (NC) and diabetic (DC) controls were given distilled water. The animals had their fasting blood glucose levels and body weights determined every 7 days for 21 days. Oral glucose tolerance tests (OGTTs) were carried out in all animals at the beginning and the end of the experiment. Liver and kidney samples were harvested for glucose 6 phosphatase (G6Pase) and hexokinase activity analyses. Small intestines and diaphragms from normal rats were used for α-glucosidase and glucose uptake studies against the extract. Results: Two doses, 150 and 300 mg/kg bw, significantly reduced the fasting blood glucose levels in diabetic rats and helped them maintain normal body weights. The glucose level in DC rats significantly increased while their body weights decreased. The 150 mg/kg bw dose significantly increased hexokinase and decreased G6Pase activities in the liver and the kidneys. ECA inhibited α-glucosidase activity and promoted glucose uptake in the rats’ hemi-diaphragms. Conclusion: This study revealed that ECA normalized blood glucose levels and body weights in type 2 diabetic rats. The normalization of the glucose levels may possibly be due to inhibition of α-glucosidase, decreased G6Pase activity, increased hexokinase activity and improved glucose uptake by muscle tissues.
Abstract
Objectives: This study aimed to evaluate the hypoglycemic effects of an ethanol extract of Cassia abbreviata (ECA) bark and the possible mechanisms of its action in diabetic albino rats. Methods: ECA was prepared by soaking the powdered plant material in 70% ethanol. It was filtered and made solvent-free by evaporation on a rotary evaporator. Type 2 diabetes was induced in albino rats by injecting 35 mg/ kg body weight (bw) of streptozotocin after having fed the rats a high-fat diet for 2 weeks. Diabetic rats were divided into ECA-150, ECA-300 and Metformin (MET)-180 groups, where the numbers are the doses in mg.kg.bw administered to the groups. Normal (NC) and diabetic (DC) controls were given distilled water. The animals had their fasting blood glucose levels and body weights determined every 7 days for 21 days. Oral glucose tolerance tests (OGTTs) were carried out in all animals at the beginning and the end of the experiment. Liver and kidney samples were harvested for glucose 6 phosphatase (G6Pase) and hexokinase activity analyses. Small intestines and diaphragms from normal rats were used for α-glucosidase and glucose uptake studies against the extract. Results: Two doses, 150 and 300 mg/kg bw, significantly reduced the fasting blood glucose levels in diabetic rats and helped them maintain normal body weights. The glucose level in DC rats significantly increased while their body weights decreased. The 150 mg/kg bw dose significantly increased hexokinase and decreased G6Pase activities in the liver and the kidneys. ECA inhibited α-glucosidase activity and promoted glucose uptake in the rats’ hemi-diaphragms. Conclusion: This study revealed that ECA normalized blood glucose levels and body weights in type 2 diabetic rats. The normalization of the glucose levels may possibly be due to inhibition of α-glucosidase, decreased G6Pase activity, increased hexokinase activity and improved glucose uptake by muscle tissues.
참고문헌 (24)
- Wild S, "Estimates for the year 2000 and projections for 2030", Diabetes Care, 27(5), pp.1047~1053 (2004)
- American Diabetes Association, "Diagnosis and classification of diabetes mellitus", Diabetes Care, 33(S1), pp.62~69 (2012)
- Shearer BG, "The next generation of PPAR drugs : do we have the tools to find them?", Biochim Biophys Acta, 1771(8), pp.1082~1093 (2007)
- Noh RM, "Medically minimising the impact of hypoglycaemia in type 2 diabetes : a review", Expert Opin Pharmacother, 12(14), pp.2161~2175 (2011)
- The effect of intensive treatment of diabetes on the development and the progression of long term complications in IDDM, "WHO Expert Committee on Diabetes Mellitus", World Health Organization, 123~, pp.Technical Report Series 646 (1980)
- Setshogo MP, "Floristic diversity and uses of medicinal plants sold by street vendors in Gaborone, Botswana", Afri. J. Plant Sci. Biotech, 5, pp.69~74 (2011)
- Venter F, "Making the most of indigenous trees", pp.92~ (2009), Briza Publications
- Leteane MM, "Old plant newly discovered: Cassia sieberiana D.C and Cassia abbreviata Oliv. root extracts inhibit in vitro HIV-1c replication in peripheral blood mononuclear cells (PBMCs) by different modes of action", J Ethnopharmacol, 141(1), pp.48~56 (2012)
- Shai LJ, "Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa", S Afr J Bot, 76(3), pp.465~470 (2010)
- Somani G, "Inhibition of carbohydrate hydrolyzing enzymes by methanolic ex-tract of Couroupita guianensis leaves", Int J Pharm Bio Sci, 3(4), pp.511~520 (2012)
- Ahmed F, "In vitro studies on the hypoglycemic potential of Ficus racemosa stem bark", J Sci Food Agric, 90(3), pp.397~401 (2010)
- Bisswanger H., "Practical enzymology", pp.69~ (2004), Wiley–VCH
- Tiwari AK, "Diabetes mellitus and multiple therapeutic approaches of phytochemicals : present status and future prospects", Curr Sci, 83(1), pp.30~38 (2002)
- Shai LJ, "Inhibitory effects of five medicinal plants on rat alpha-glucosidase : comparison with their effects on yeast alpha-glucosidase", J Med Plants Res, 5(13), pp.2863~2867 (2011)
- Ferrier DR, "Lippincott’s illustrated reviews: biochemistry", pp.53~ (2011), Lippincott Williams and Wilkins
- Richter EA, "Exercise, GLUT4 and skeletal muscle glucose uptake", Physiol Rev, 93(3), pp.993~1017 (2013)
- Erasto P, "Bioactive proanthocyanidins from the root bark of Cassia abbreviata", Int J Biol Chem Sci, 5(5), pp.2170~2179 (2011)
- Mongalo NI, "Antibacterial activities of selected medicinal plants used to treat sexually transmitted infections in Blouberg area, Limpopo Province", pp.2013
- Mithieux G, "Intrahepatic mechanisms underlying the effect of metformin in decreasing basal glucose production in rats fed a high fat diet", Diabetes, 51(1), pp.139~143 (2002)
- Mallick C, "Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Coccinia indica in streptozotocin-induced diabetic male albino rat", Afr J Tradit Complement Altern Med, 4(3), pp.362~371 (2007)
- Arion JW, "Direct evidence for the involvement of two glucose 6 phosphate binding sites in the glucose 6phosphatase activity of intact liver microsomes", J Biol Chem, 273(11), pp.6223~6227 (1998)
- Bhandari U, "Antidiabetic effects of Embelia ribes extract in high fat diet and low dose streptozotocin-induced type 2 diabetic rats", Front Life Sci, 7(3), pp.186~196 (2013)
- Massa ML, "Liver glucokinase : an overview on the regulatory mechanisms of its activity", IUBMB Life, 63(1), pp.1~6 (2011)
- Torres TP, "Restoration of hepatic glucokinase expression corrects hepatic glucose flux and normalizes plasma glucose in zucker diabetic fatty rats", Diabetes, 58(1), pp.78~86 (2009)