Biocompatibility and Bioactivity of Four Different Root Canal Sealers in Osteoblastic Cell Line MC3T3-El
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Endodontic sealers or their toxic components may become inflamed and lead to delayed wound healing when in direct contact with periapical tissues over an extended period. Moreover, an overfilled sealer can directly interact with adjacent tissues and may cause immediate necrosis or further resorption. Therefore, the treatment outcome conceivably depends on the endodontic sealer’s biocompatibility and osteogenic potential. This study aimed to evaluate the cell viability and osteogenic effects of four different sealers in osteoblastic cells. Methods: AH Plus (resin-based sealer), Pulp Canal Sealer EWT (zinc oxide-eugenol sealer), BioRoot RCS (calcium silicate-based sealer), and Well-Root ST (MTA-based calcium silicate sealer) were mixed strictly according to the manufacturer’s instructions, and dilutions of sealer extracts (1/2, 1/5 and 1/10) were determined. Cell viability was measured using the water-soluble tetrazolium-8 (WST-8) assay. Differentiation was assessed by alkaline phosphatase (ALP) activity and mineralized nodule formation by Alizarin Red S staining. Results: The cell viability of the extracts derived from the sealers excluding Well-Root ST was concentration dependent, with sealer extracts having the least viability at a 1/2 dilution. At sealer extract dilution of 1/10, the test groups showed the same survival rate as that control group, with the exception of BioRoot RCS. Among all experimental groups, BioRoot RCS showed the highest cell viability after 48 hours. The ALP activity was significantly higher in a concentration-dependent manner. Furthemore, all four materials promoted ALP activity and mineralized nodule formation compared to the control at 1/10 dilutions. Conclusion: This is the first study to highlight the differences in biological activity of these four materials. These results suggest that the composition of root canal sealers appears to alter the form of biocompatibility and osteoblastic differentiation.
Abstract
Background: Endodontic sealers or their toxic components may become inflamed and lead to delayed wound healing when in direct contact with periapical tissues over an extended period. Moreover, an overfilled sealer can directly interact with adjacent tissues and may cause immediate necrosis or further resorption. Therefore, the treatment outcome conceivably depends on the endodontic sealer’s biocompatibility and osteogenic potential. This study aimed to evaluate the cell viability and osteogenic effects of four different sealers in osteoblastic cells. Methods: AH Plus (resin-based sealer), Pulp Canal Sealer EWT (zinc oxide-eugenol sealer), BioRoot RCS (calcium silicate-based sealer), and Well-Root ST (MTA-based calcium silicate sealer) were mixed strictly according to the manufacturer’s instructions, and dilutions of sealer extracts (1/2, 1/5 and 1/10) were determined. Cell viability was measured using the water-soluble tetrazolium-8 (WST-8) assay. Differentiation was assessed by alkaline phosphatase (ALP) activity and mineralized nodule formation by Alizarin Red S staining. Results: The cell viability of the extracts derived from the sealers excluding Well-Root ST was concentration dependent, with sealer extracts having the least viability at a 1/2 dilution. At sealer extract dilution of 1/10, the test groups showed the same survival rate as that control group, with the exception of BioRoot RCS. Among all experimental groups, BioRoot RCS showed the highest cell viability after 48 hours. The ALP activity was significantly higher in a concentration-dependent manner. Furthemore, all four materials promoted ALP activity and mineralized nodule formation compared to the control at 1/10 dilutions. Conclusion: This is the first study to highlight the differences in biological activity of these four materials. These results suggest that the composition of root canal sealers appears to alter the form of biocompatibility and osteoblastic differentiation.
참고문헌 (27)
- Domenico Ricucci, "Apically Extruded Sealers: Fate and Influence on Treatment Outcome", Journal of Endodontics, 42(2), pp.243~249 (2016), Elsevier BV[DOI]
- Giuseppe TROIANO, "<i>In vitro<", i> evaluation of the cytotoxic activity of three epoxy resin-based endodontic sealers, 2018, pp.37(3), 374~378[DOI]
- Akhil Chandra, "Discuss the factors that affect the outcome of endodontic treatment", Australian Endodontic Journal, 35(2), pp.98~107 (2009), Wiley[DOI]
- M. Bernáth, "Tissue reaction initiated by different sealers", International Endodontic Journal, 36(4), pp.256~261 (2003), Wiley[DOI]
- Roberto Holland, "Factors affecting the periapical healing process of endodontically treated teeth", Journal of Applied Oral Science, 25(5), pp.465~476 (2017), FapUNIFESP (SciELO)[DOI]
- S Ghanaati, "Tissue reaction to sealing materials: different view at biocompatibility", European Journal of Medical Research, 15(11), pp.483~492 (2010), Springer Science and Business Media LLC[DOI]
- Alejandro Victoria-Escandell, "Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials", Stem Cells International, 2017, pp.1~14 (2017), Hindawi Limited[DOI]
- M. Almeida, "Analysis of the physicochemical properties, cytotoxicity and volumetric changes of AH Plus, MTA Fillapex and TotalFill BC Sealer", Journal of Clinical and Experimental Dentistry, 12, pp.e1058~e1065 (2020), Medicina Oral, S.L.[DOI]
- Ines Willershausen, "In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers", Head & Face Medicine, 7(1), pp.15~ (2011), Springer Science and Business Media LLC[DOI]
- Bethany A. Loushine, "Setting Properties and Cytotoxicity Evaluation of a Premixed Bioceramic Root Canal Sealer", Journal of Endodontics, 37(5), pp.673~677 (2011), Elsevier BV[DOI]
- João E. Gomes-Filho, "Evaluation of the biocompatibility of root canal sealers using subcutaneous implants", Journal of Applied Oral Science, 15(3), pp.186~194 (2007), FapUNIFESP (SciELO)[DOI]
- Vassiliki Taraslia, "Assessment of cell viability in four novel endodontic sealers", European Journal of Dentistry, 12(02), pp.287~291 (2018), Georg Thieme Verlag KG[DOI]
- Jung S, "Cytotoxic effects of four different root canal sealers on human osteoblasts", PLoS One, 13, pp.e0194467~ (2018)[DOI]
- Taddei P, "Vibrational study on the bioactivity of Portland cement-based materials for endodontic use", J Mol Struct, 924-926, pp.548~554 (2009)[DOI]
- João Eduardo Gomes-Filho, "A Mineral Trioxide Aggregate Sealer Stimulated Mineralization", Journal of Endodontics, 35(2), pp.256~260 (2009), Elsevier BV[DOI]
- W. Geurtsen, "Biological aspects of root canal filling materials - histocompatibility, cytotoxicity, and mutagenicity", Clinical Oral Investigations, 1(1), pp.5~11 (1997), Springer Science and Business Media LLC[DOI]
- Lodiene G, "Toxicity evaluation of root canal sealers in vitro", Int Endod J, 41, pp.72~77 (2008)[DOI]
- Deog-Gyu Seo, "Biocompatibility and Mineralization Activity of Three Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Dental Pulp Stem Cells", Materials, 12(15), pp.2482~ (2019), MDPI AG[DOI]
- 김희정, "레진 계열 근관봉함재 Adseal의 세포독성에 관한 연구", Restorative Dentistry and Endodontics, 29(6), pp.498~503 (2004), 대한치과보존학회
- H. Schweikl, "Mutagenicity of the root canal sealer AHPlus in the Ames test", Clinical Oral Investigations, 2(3), pp.125~129 (1998), Springer Science and Business Media LLC[DOI]
- Hubbe KL, "AH Plus extrusion into periapical tissue: literature review of main related properties and report of clinical cases", Revis Sul-Bras Odontol, 13, pp.280~288 (2016)
- Lee JK, "In vitro comparison of biocompatibility of calcium silicate-based root canal sealers", Materials (Basel), 12, pp.2411~ (2019)[DOI]
- David Donnermeyer, "Endodontic sealers based on calcium silicates: a systematic review", Odontology, 107(4), pp.421~436 (2019), Springer Science and Business Media LLC[DOI]
- C. L. Zordan‐Bronzel, "Cytocompatibility, bioactive potential and antimicrobial activity of an experimental calcium silicate‐based endodontic sealer", International Endodontic Journal, 52(7), pp.979~986 (2019), Wiley[DOI]
- Satoshi Imazato, "Responses of MC3T3-E1 cells to three dental resin-based restorative materials", Journal of Biomedical Materials Research Part A, 76A(4), pp.765~772 (2006), Wiley[DOI]
- Mukaddes Gulec, "Vitamin E Protects Against Oxidative Damage Caused by Formaldehyde in the Liver and Plasma of Rats", Molecular and Cellular Biochemistry, 290(1-2), pp.61~67 (2006), Springer Science and Business Media LLC[DOI]
- Fu-Mei Huang, "Effects of Root Canal Sealers on Alkaline Phosphatase in Human Osteoblastic Cells", Journal of Endodontics, 36(7), pp.1230~1233 (2010), Elsevier BV[DOI]