A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929). Materials and Methods Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis. Results Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity. Conclusions Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.
Abstract
Objectives The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929). Materials and Methods Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis. Results Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity. Conclusions Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.
참고문헌 (38)
- Ingle JI, "Endodontics, In Obturation of the radicular space", pp.1053~1087 (2008), Decker Inc
- Grossman LI, "Endodontic practice, In Obturation of radicular space", pp.278~309 (2010), Walter Kluwer Pvt. Ltd
- De Deus QD, "Frequency, location, and direction of lateral, secondary, and accessory canals", J Endod, 1, pp.361~366 (1975)
- Dongari A, "Periodontally derived pulpal lesions", Endod Dent Traumatol, 4, pp.49~54 (1988)
- Dahl JE, "In vitro biocompatibility of denture relining materials", Gerodontology, 23, pp.17~22 (2006)
- Miletić I, "The cytotoxicity of Roeko Seal and AH plus compared during different setting periods", J Endod, 31, pp.307~309 (2005)
- Al-Nazhan S, "Morphological cell changes due to chemical toxicity of a dental material: an electron microscopic study on human periodontal ligament fibroblasts and L929 cells", J Endod, 16, pp.129~134 (1990)
- "ISO-Standards ISO 10993. Biological compatibility of medical devices. Test for cytotoxicity: in vitro methods" (2009)[URL]
- Leonardo RT, "Evaluation of cell culture cytotoxicity of five root canal sealers", J Endod, 26, pp.328~330 (2000)
- Huang FM, "Induction of c-fos and c-jun protooncogenes expression by formaldehyde-releasing and epoxy resin-based rootcanal sealers in human osteoblastic cells", J Biomed Mater Res, 59, pp.460~465 (2002)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
- Oztan MD, "A comparison of the in vitro cytotoxicity of two root canal sealers", J Oral Rehabil, 30, pp.426~429 (2003)
- Koulaouzidou EA, "Cytotoxicity of three resin-based root canal sealers: an in vitro evaluation", Endod Dent Traumatol, 14, pp.182~185 (1998)
- Pinna L, "In vitro cytotoxicity evaluation of a self-adhesive, methacrylate resin-based root canal sealer", J Endod, 34, pp.1085~1088 (2008)
- Osorio RM, "Cytotoxicity of endodontic materials", J Endod, 24, pp.91~96 (1998)
- Azar NG, "In vitro cytotoxicity of a new epoxy resin root canal sealer", J Endod, 26, pp.462~465 (2000)
- Miletić I, "Cytotoxic effect of four root filling materials", Endod Dent Traumatol, 16, pp.287~290 (2000)
- Geursten W, "Cytotoxicity of four root canal sealers in permanent 3T3 cells and primary human periodontal ligament fibroblast cultures", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, pp.592~597 (1998)
- Nakamura H, "Study on cytotoxicity of root canal filling materials", J Endod, 12, pp.156~160 (1986)
- Guigand M, "Evaluation of the cytotocompatibility of three endodontic materials", J Endod, 25, pp.419~423 (1999)
- Gerosa R, "Cytotoxicity evaluation of six root canal sealers", J Endod, 21, pp.446~448 (1995)
- Schwarze T, "The cellular compatibility of five endodontic sealers during the setting period", J Endod, 28, pp.784~786 (2002)
- Tornek CD, "The effect of calcium hydroxide on porcine pulp fibroblasts in vitro", J Endod, 9, pp.131~136 (1983)
- Swierenga SH, "Regulation by calcium of the proliferation of heart cells from young adult rats", In Vitro, 12, pp.31~36 (1976)
- Whitfield JF, "The positive control of cell proliferation by the interplay on calcium ions and cyclic nucleotides. A review", In Vitro, 12, pp.1~18 (1976)
- 김창규, "레진계 근관충전실러의 방사선 불투과성 및 세포 독성에 대한 평가", Restorative Dentistry and Endodontics, 32(5), pp.419~425 (2007), 대한치과보존학회
- Schwarze T, "Long-term cytocompatibility of various endodontic sealers using a new root canal model", J Endod, 28, pp.749~753 (2002)
- 김희정, "신개발 인산칼슘계 근관 봉함재의 세포독성 및 유전독성에 관한 연구", Restorative Dentistry and Endodontics, 31(1), pp.36~49 (2006), 대한치과보존학회
- 박소영, "새로운 레진 계통의 근관봉함재의 독성과 항균 작용에 대한 연구", Restorative Dentistry and Endodontics, 28(2), pp.162~168 (2003), 대한치과보존학회
- Cohen BI, "An in vitro study of the cytotoxicity of two root canal sealers", J Endod, 26, pp.228~229 (2000)
- Vajrabhaya L, "Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers", Int Endod J, 30, pp.141~144 (1997)
- Spångberg LS, "AH-26 releases formaldehyde", J Endod, 19, pp.596~598 (1993)
- Kaplan AE, "Disintegration of endodontic cements in water", J Endod, 23, pp.439~441 (1997)
- Araki K, "Reduced cytotoxicity of a root canal sealer through eugenol substitution", J Endod, 19, pp.554~557 (1993)
- Key JE, "Cytotoxicity of a new root canal filling material on human gingival fibroblasts", J Endod, 32, pp.756~758 (2006)
- Watts A, "Cellular responses in the dental pulp: a review", Int Endod J, 14, pp.10~19 (1981)
- Wataha JC, "Cytotoxicity of components of resins and other dental restorative materials", J Oral Rehabil, 21, pp.453~462 (1994)
- Wiegand C, "Methods for the measurement of cell and tissue compatibility including tissue regeneration processes", GMS Krankenhhyg Interdiszip, 3, pp.Doc 12~ (2008)