龍膽瀉肝湯加味方 3種의 염증관련 인자 억제에 관한 연구
Suppressive Effect of Yongdamsagantanggamibang on the Inflammatory Factors
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Purpose: The purpose of this study is to investigate the anti-inflammatory effects of three types of Yongdamsagantanggamibang(YSTG) which has been medicated the patient with inflammatory disease of female genitourinary system. Methods: To verify the anti-inflammatory mechanism of YSTGs, expressions of IL-1β, IL-6, MCP-1, COX-2 and TNF-α mRNA in THP-1 cells were examined. And we investigated the production levels of IL-1β, IL-6 and TNF-α in mouse following LPS co-treatment. Results: 1. YSTG1, YSTG2 and YSTG3 extract did not show any cytotoxic effect on human fibroblast cells at any of the concentrations evaluated(500, 250, 125, 62.5, 37.25 ㎍/㎖) 2. YSTG1, YSTG2 and YSTG3 extract showed scavenging activity on DPPH free radical and SOD-like activity. 3. YSTG1, YSTG2 and YSTG3 extract decreased production levels of IL-1β, IL-6, IL-8, TNF-α and MCP-1 in LPS-treated THP-1 cells. 4. YSTG1, YSTG2 and YSTG3 extract decreased expressions of IL-1β, IL-6, MCP-1, COX-2 and TNF-α mRNA in LPS-treated THP-1 cells. 5. YSTG1, YSTG2 and YSTG3 extract decreased production levels of IL-1β, IL-6 and TNF-α in serum of LPS-treated mouse. Conclusion: Based on results above, it is revealed three types of YSTG have the anti-inflammatory effect, and may be effective in the treatment for inflammatory disease of female genitourinary system.
Abstract
Purpose: The purpose of this study is to investigate the anti-inflammatory effects of three types of Yongdamsagantanggamibang(YSTG) which has been medicated the patient with inflammatory disease of female genitourinary system. Methods: To verify the anti-inflammatory mechanism of YSTGs, expressions of IL-1β, IL-6, MCP-1, COX-2 and TNF-α mRNA in THP-1 cells were examined. And we investigated the production levels of IL-1β, IL-6 and TNF-α in mouse following LPS co-treatment. Results: 1. YSTG1, YSTG2 and YSTG3 extract did not show any cytotoxic effect on human fibroblast cells at any of the concentrations evaluated(500, 250, 125, 62.5, 37.25 ㎍/㎖) 2. YSTG1, YSTG2 and YSTG3 extract showed scavenging activity on DPPH free radical and SOD-like activity. 3. YSTG1, YSTG2 and YSTG3 extract decreased production levels of IL-1β, IL-6, IL-8, TNF-α and MCP-1 in LPS-treated THP-1 cells. 4. YSTG1, YSTG2 and YSTG3 extract decreased expressions of IL-1β, IL-6, MCP-1, COX-2 and TNF-α mRNA in LPS-treated THP-1 cells. 5. YSTG1, YSTG2 and YSTG3 extract decreased production levels of IL-1β, IL-6 and TNF-α in serum of LPS-treated mouse. Conclusion: Based on results above, it is revealed three types of YSTG have the anti-inflammatory effect, and may be effective in the treatment for inflammatory disease of female genitourinary system.
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