銀甲方이 염증 관련 cytokines의 유전자 발현과 생성량에 미치는 영향
The Experimental Study on Anti-inflammatory Effects of Eungapbang(EGB)
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Purpose: This study was performed to evaluate the anti-inflammatory effect of Eungapbang extract (EGB). Methods: To evaluate the anti-inflammatory effects of EGB, we nourished RAW 264.7 cell lines in the laboratory dish. Next, inflammatory cytokine concentrations were analyzed. Then, sera were prepared from blood after lipopolysaccharide (LPS) injection in chemically induced mouse models of intestinal inflammation, and Interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-α) were measured using ELISA kits. Results: 1. EGB significantly suppressed the expression levels of IL-1β and NOS-Ⅱ genes at 100, 50 and 10 ㎍/㎖ concentrations, and IL-6 , TNF-α and COX-2 mRNAs at 100 and 50 ㎍/㎖ concentrations. 2. EGB significantly reduced the production level of IL-1β and TNF-α at 100 ㎍/㎖ concentrations, and IL-6 at 100 and 50 ㎍/㎖ concentrations. 3. EGB significantly decreased the production level of IL-1β and IL-6 in sera of acute inflammation induced mice. 4. EGB could suppress the expression level of IL-1β and IL-6 mRNA in spleen tissues in acute inflammation induced mice. Conclusion: On the basis of the above results, it is confirmed that the anti-inflammatory effects of EGB were recognized. Therefore, EGB is recommended as promising therapy for treatment of such ailments as pelvic inflammatory disease.
Abstract
Purpose: This study was performed to evaluate the anti-inflammatory effect of Eungapbang extract (EGB). Methods: To evaluate the anti-inflammatory effects of EGB, we nourished RAW 264.7 cell lines in the laboratory dish. Next, inflammatory cytokine concentrations were analyzed. Then, sera were prepared from blood after lipopolysaccharide (LPS) injection in chemically induced mouse models of intestinal inflammation, and Interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-α) were measured using ELISA kits. Results: 1. EGB significantly suppressed the expression levels of IL-1β and NOS-Ⅱ genes at 100, 50 and 10 ㎍/㎖ concentrations, and IL-6 , TNF-α and COX-2 mRNAs at 100 and 50 ㎍/㎖ concentrations. 2. EGB significantly reduced the production level of IL-1β and TNF-α at 100 ㎍/㎖ concentrations, and IL-6 at 100 and 50 ㎍/㎖ concentrations. 3. EGB significantly decreased the production level of IL-1β and IL-6 in sera of acute inflammation induced mice. 4. EGB could suppress the expression level of IL-1β and IL-6 mRNA in spleen tissues in acute inflammation induced mice. Conclusion: On the basis of the above results, it is confirmed that the anti-inflammatory effects of EGB were recognized. Therefore, EGB is recommended as promising therapy for treatment of such ailments as pelvic inflammatory disease.
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