梔子가 대식세포의 Cytokine에 미치는 영향
Effects of Gardeniae Fructus on Cytokines in Mouse Macrophage
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초록
Objectives: The purpose of this study was to investigate the effects of GardeniaeFructus Water Extract (GF) on the production of inflammatory mediators in RAW264.7 cell treated with lipopolysaccharide (LPS). Methods: Gradeniae Fructus was extracted with distilled water (2,000 ml) for 2hours. In order to evaluate cytotoxicity of GF, 3 - (4,5-dimethylthiazol-2-yl) - 2,5- diphenyltetrazolium bromide (MTT) assay was performed. To investigate antiinflammatoryeffects, the concentration of nitric oxide (NO) was measured with Noassay, calcium (Ca) was measured with Fluo-4 Ca assay, and cytokine was measuredby Bio-Plex cytokine assay in RAW 264.7 cell. And when p-value is below 0.05, itis judged to have the significant difference statistically. Results:1. GF did not show any cytotoxicity. 2. GF suppressed the production of NO and Ca at the concentration of 25, 50,100 and 200 μg/ml. 3. GF suppressed the production of interleukin (IL)-1β, IL-10, IL-12p40, macrophage-colony stimulating factor (M-CSF), macrophage inflammatory protein (MIP)-1β andkeratinocyte chemoattractant(KC) at the concentration of 25, 50, 100 and 200 μg/ml. 4. GF suppressed the production of vascular endothelial growth factor (VEGF),granulocyte-colony stimulating factor (G-CSF) and monocyte cheomattractant protein(MCP)-1 at the concentration of 25, 50 and 100 μg/ml. 5. GF suppressed the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF) and regulated on activation, normal T cell expressed and secreted(RANTES) at the concentration of 25 and 50 μg/ml. 6. GF suppressed the production of MIP-2 at the concentration of 50 and 100μg/ml, and tumor necrosis factor (TNF)-α at the concentration of 50 and 200 μg/ml. Conclusions: These results suggest that GF has anti-inflammatory effect andimmuno-modulating activity.
Abstract
Objectives: The purpose of this study was to investigate the effects of GardeniaeFructus Water Extract (GF) on the production of inflammatory mediators in RAW264.7 cell treated with lipopolysaccharide (LPS). Methods: Gradeniae Fructus was extracted with distilled water (2,000 ml) for 2hours. In order to evaluate cytotoxicity of GF, 3 - (4,5-dimethylthiazol-2-yl) - 2,5- diphenyltetrazolium bromide (MTT) assay was performed. To investigate antiinflammatoryeffects, the concentration of nitric oxide (NO) was measured with Noassay, calcium (Ca) was measured with Fluo-4 Ca assay, and cytokine was measuredby Bio-Plex cytokine assay in RAW 264.7 cell. And when p-value is below 0.05, itis judged to have the significant difference statistically. Results:1. GF did not show any cytotoxicity. 2. GF suppressed the production of NO and Ca at the concentration of 25, 50,100 and 200 μg/ml. 3. GF suppressed the production of interleukin (IL)-1β, IL-10, IL-12p40, macrophage-colony stimulating factor (M-CSF), macrophage inflammatory protein (MIP)-1β andkeratinocyte chemoattractant(KC) at the concentration of 25, 50, 100 and 200 μg/ml. 4. GF suppressed the production of vascular endothelial growth factor (VEGF),granulocyte-colony stimulating factor (G-CSF) and monocyte cheomattractant protein(MCP)-1 at the concentration of 25, 50 and 100 μg/ml. 5. GF suppressed the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF) and regulated on activation, normal T cell expressed and secreted(RANTES) at the concentration of 25 and 50 μg/ml. 6. GF suppressed the production of MIP-2 at the concentration of 50 and 100μg/ml, and tumor necrosis factor (TNF)-α at the concentration of 50 and 200 μg/ml. Conclusions: These results suggest that GF has anti-inflammatory effect andimmuno-modulating activity.
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