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女貞實이 LPS로 유발된 RAW 264.7 cell의 염증에 미치는 영향

Anti-Inflammatory Effect of Ligustri Lucidi Fructus Water Extract in RAW 264.7 Cells Induced by LPS

임은미|이용현
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2013.11|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제26권 제4호|pp. 66-81

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키워드

NOAnti-inflammationCytokineLigustri Lucidi FructusCa
주제분류한의학

초록

Purpose: This study was carried out to investigate the anti-inflammatory effectsof Ligustri Lucidi Fructus water extract (LF) in the lipopolysaccharide (LPS)-inducedmouse macrophages RAW 264.7 cell. Methods: Ligustri Lucidi Fructus was extracted with distilled water (2,000 ml)for 2 hours. In order to evaluate cytotoxicity of LF, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. To investigateanti-inflammatory effects of LF, the concentration of nitric oxide (NO) was measuredwith NO assay, cytokine was measured by Bio-Plex cytokine assay, and intracellularcalcium (Ca) was measured with Fluo-4 Ca assay in RAW 264.7 cell. And when p-valueis below 0.05, it is judged to have the significant difference statistically (P<0.05). Results:1. LF showed no cytotoxicity. 2. LF inhibited significantly the production of NO at the concentration of 25,50 and 100 μg/ml. 3. LF inhibited significantly the production of interleukin (IL)-4, macrophageinflammatory protein (MIP)-1α, granulocyte colony stimulating factor (G-CSF)at the concentration of 25, 50, 100 and 200 μg/ml. 4. LF inhibited significantly the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF), vascular endothelial growth factor (VEGF) at the concentrationof 50, 100 and 200 μg/ml, the interferon (IFN)-γ at 25, 50 and 100 μg/ml respectively. 5. LF inhibited significantly the production of IL-1β at the concentration of 50and 200 μg/ml, the IL-5 at 25 and 100 μg/ml, the IL-12p70, MIP-1β at 50 and100 μg/ml, the regulated on activation, normal T cell expressed and secrete d(RANTES) at 100 and 200 μg/ml respectively. 6. LF inhibited significantly the production of IL-10, interferon gamma-inducedprotein (IP)-10 at the concentration of 200 μg/ml. 7. LF inhibited significantly the production of intracellular Ca at the concentrationof 25, 50, 100 and 200 μg/ml. Conclusions: These results suggest that LF has anti-inflammatory effect andimmuno-modulating activity.

Abstract

Purpose: This study was carried out to investigate the anti-inflammatory effectsof Ligustri Lucidi Fructus water extract (LF) in the lipopolysaccharide (LPS)-inducedmouse macrophages RAW 264.7 cell. Methods: Ligustri Lucidi Fructus was extracted with distilled water (2,000 ml)for 2 hours. In order to evaluate cytotoxicity of LF, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. To investigateanti-inflammatory effects of LF, the concentration of nitric oxide (NO) was measuredwith NO assay, cytokine was measured by Bio-Plex cytokine assay, and intracellularcalcium (Ca) was measured with Fluo-4 Ca assay in RAW 264.7 cell. And when p-valueis below 0.05, it is judged to have the significant difference statistically (P<0.05). Results:1. LF showed no cytotoxicity. 2. LF inhibited significantly the production of NO at the concentration of 25,50 and 100 μg/ml. 3. LF inhibited significantly the production of interleukin (IL)-4, macrophageinflammatory protein (MIP)-1α, granulocyte colony stimulating factor (G-CSF)at the concentration of 25, 50, 100 and 200 μg/ml. 4. LF inhibited significantly the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF), vascular endothelial growth factor (VEGF) at the concentrationof 50, 100 and 200 μg/ml, the interferon (IFN)-γ at 25, 50 and 100 μg/ml respectively. 5. LF inhibited significantly the production of IL-1β at the concentration of 50and 200 μg/ml, the IL-5 at 25 and 100 μg/ml, the IL-12p70, MIP-1β at 50 and100 μg/ml, the regulated on activation, normal T cell expressed and secrete d(RANTES) at 100 and 200 μg/ml respectively. 6. LF inhibited significantly the production of IL-10, interferon gamma-inducedprotein (IP)-10 at the concentration of 200 μg/ml. 7. LF inhibited significantly the production of intracellular Ca at the concentrationof 25, 50, 100 and 200 μg/ml. Conclusions: These results suggest that LF has anti-inflammatory effect andimmuno-modulating activity.

참고문헌 (56)

  1. 김각균, "최신 면역학", pp.1~39 (2005), 범문사
  2. 대한병리학회, "병리학", pp.67~94 (2003), 고문사
  3. 송계용, "핵심 병리학", pp.63~64 (1998), 고려의학
  4. 고려대학교 의과대학 병리학교실, "병리학", pp.28~31 (2001), 신광출판사
  5. Wu MJ, "Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage cell line by downregulating LPS-induced NF-κB", J Biomed Sci, 11(2), pp.186~199 (2004)
  6. Dinarello CA, "Interleukin-1, interleukin-1 receptors and interleukin-1 receptor antagonist", Int Rev Immunol, 16(5-6), pp.457~499 (1998)
  7. Nathan C, "Natural resistance and nitric oxide", Cell, 82(6), pp.873~876 (1995)
  8. Fenton MJ, "LPS-binding proteins and receptors", J Leukoc Biol, 64(1), pp.25~32 (1998)
  9. Glauser MP, "The inflammatory cytokines. New developments in the pathophysiology and treatment of septic shock", Drugs, 52(2), pp.9~17 (1996)
  10. 서부일, "본초학", pp.937~939 (2012), 영림사
  11. 이광석, "여정자가 대식세포 탐식능에 미치는 영향", 대한한의학회지, 17(2), pp.227~236 (1996)
  12. 송제문, "활성화된 대식세포로부터 일산화질소 및 염증성 세포활성물질들의 생성에 대한 여정자의 효과", 석사, 한의학, pp.2004
  13. 전춘산, "이지환과 그 구성약재의 항산화 및 항염증 효과", 박사, 한의학, pp.2009
  14. 나유진, "H₂O₂에 노출된 LLC-PK₁ 세포에서 Redox Status 및 NF-κB Signaling에 미치는 여정자의 효과", 석사, 한의학, pp.2009
  15. Politch JA, "Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men", Hum Reprod, 22(11), pp.2928~2935 (2007)
  16. Lee KS, "Bioplex analysis of plasma cytokines in Alzheimer's disease and mild cognitive impairment", Immunol Lett, 121(2), pp.105~109 (2008)
  17. Guo LY, "Anti-inflammatory effects of schisandrin isolated from the fruit of schisandra chinensis baill", Eur J Pharmacol, 591(1-3), pp.293~299 (2008)
  18. Bosca L, "Nitric oxide and cell viability in inflammatory cells : a role for NO in macrophage function and fate", Toxicology, 208(2), pp.249~258 (2005)
  19. Nathan C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6(12), pp.3051~3064 (1992)
  20. Wei W, "Anti-inflammatory and immunopharmacology", pp.10~17 (2004), Renmin weisheng chubanshe
  21. Turini ME, "Cyclooxygenase-2: a therapeutic target", Annu Rev Med, 53, pp.35~57 (2002)
  22. Tilg H, "Serum levels of cytokines in chronic liver diseases", Gastroenterology, 103(1), pp.264~274 (1992)
  23. Libby P, "Inflammation and atherosclerosis", Circulation, 105(9), pp.1135~1143 (2002)
  24. Kim KM, "Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-kappaB activation", Toxicol Appl pharmacol, 190(1), pp.1~8 (2003)
  25. 염미정, "RAW 264.7 면역세포에서 염증유발인자의 유전자 발현에 대한 軟玉水와 軟玉粉의 억제 효과", 동의생리병리학회지, 18(6), pp.1622~1627 (2004), 대한동의병리학회
  26. Greenstein B, "The endocrine system at a glance", pp.98~101 (2008), Blackwell publishing Ltd
  27. Lefkowith JB, "Cyclooxygenase-2 specificity and its clinical implications", American Journal of Medicine, 106(5), pp.43~50 (1999)
  28. Solomon SD, "Cyclooxygenase-2 inhibitors and cardiovascular risk", Curr Opin Cardiol, 21(6), pp.613~617 (2006)
  29. 홍원식, "精校黃帝內經素問", pp.38~ (1985), 동양의학연구원출판사
  30. 홍원식, "精校黃帝內經靈樞", pp.317~ (1981), 동양의학연구원출판사
  31. 채우석, "면역질환의 한방개념과 치료에 관한 문헌적 고찰", 대한한의학회지, 11(2), pp.54~58 (1990)
  32. 조종관, "면역에 관한 동양의학적 고찰", 동양의학, 12(1), pp.19~23 (1986)
  33. 吳普, "孫星衍 孫馮翼 輯錄. 神農本草經", pp.43~ (1982), 人民衛生出版社
  34. 송석복, "여정실류의 효능에 관한 비교연구", 본초분류학회지, 6(1), pp.13~19 (1991)
  35. McCann SM, "The nitric oxide theory of aging revisited", Ann N Y Acad Sci, 1057, pp.64~84 (2005)
  36. Roitt I, "Immunology", pp.119~ (2001), Mosby
  37. Brandt EB, "Th2 Cytokines and Atopic Dermatitis", J Clin Cell Immunol, 2(3), pp.110~124 (2011)
  38. van Driel WJ, "Presence of an eosinophilic infiltrate in cervical squamous carcinoma results from a type 2 immune response", Gynecol Oncol, 74(2), pp.188~195 (1999)
  39. Goldsby RA, "Immunology", pp.281~ (2005), WH Freeman and Company
  40. Trinchieri G, "Interleukin-12 and the regulation of innate resistance and adaptive immunity", Nat Rev Immunol, 3(2), pp.133~146 (2003)
  41. Maurer M, "Macrophage inflammatory protein-1", Int J Biochem Cell Biol, 36(10), pp.1882~1886 (2004)
  42. Irving SG, "Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q", Nucleic Acids Res, 18(11), pp.3261~3270 (1990)
  43. Ren M, "Polymerization of MIP-1 chemokine(CCL3 and CCL4) and clearance of MIP-1 by insulin -degrading enzyme", EMBO J, 29(23), pp.3952~3966 (2010)
  44. Maghazachi AA, "CC chemokines induce the generation of killer cells from CD56+ cells", Eur J Immunol, 26(2), pp.315~319 (1996)
  45. Luster AD, "Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins", Nature, 315(6021), pp.672~676 (1985)
  46. Agostini C, "Involvement of the IP-10 chemokine in sarcoid granulomatous reactions", J immunol, 161(11), pp.6413~6420 (1998)
  47. Agostini C, "CXC chemokines IP-10 and mig expression and direct migration of pulmonary CD8+", CXCR3+ T cells in the lungs of patients with HIV infection and T-cell alveolitis, 2000, pp.162(4), 1466~1473
  48. Richards MK, "Pivotal role of granulocyte colony-stimulating factor in the development of progenitors in the common myeloid pathway", Blood, 102(10), pp.3562~3568 (2003)
  49. Barreda DR, "Regulation of myeloid development and function by colony stimulating factors", Dev Comp Immunol, 28(5), pp.509~554 (2004)
  50. Zhu QS, "G-CSF induced reactive oxygen species involves Lyn-PI3-kinase-Akt and contributes to myeloid cell growth", Blood, 107(5), pp.1847~1856 (2006)
  51. Morikawa K, "Characterization of granulocyte colony-stimulating factor receptor expressed on human lymphocytes", Br J Haematol, 118(1), pp.296~304 (2002)
  52. Geijsen N, "Specificity in cytokine signal transduction: lessons learned from the IL-3", IL-5, Cytokine Growth Factor Rev, pp.2001, 12(1)[DOI]
  53. Tischer E, "Vascular endothelial growth factor : a new member of the platelet-derived growth factor gene family", Biochem Biophys Res Commun, 165(3), pp.1198~1206 (1989)
  54. Mire-Sluis AR, "Cytokines", pp.19~400 (1998), Academic Press
  55. Martin L, "Activation of the adenosine A3 receptor in RAW 264.7 cells inhibits lipopolysaccharide-stimulated tumor necrosis factor-alpha release by reducing calcium-dependent activation of nuclear factor-kappaB and extracellular signal-regulated kinase 1", 2, 2006, pp.316(1), 71~78
  56. Fehsel K, "Nitric oxide induces apoptosis in mouse thymocytes", J Immunol, 155(6), pp.2858~2865 (1995)

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