五積散 경구투여에 의한 면역활성과 종양 전이 억제 효과
Activation of Immune System & Antimetastatic Effects of Ojeok-san by Oral Administration
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives: This study was designed to investigate intestinal immune systemactivation and antimetastatic effect of Ojeok-san on cancer cells by oral administration. Methods: Cell viability of Ojeok-san was tested with colon 26-M3.1 carcinomacells and Peyer's patch cells in vitro. Antimetastatic experiments were conductedin vivo mouse model by using colon 26-M3.1 carcinoma cell. To observe immunomodulatingeffects of Ojeok-san on Peyer's patch cells, we measured interleukin (IL)-4, GM-CSF. In addition to observing effects of Ojeok-san on hematopoiesis, we measured proliferationof bone marrow cells mediated by Peyer's patch cells in vitro. IgA induction activated in serum and intestinal content was measured to observethe effect of orally administered Ojeok-san on mucosal immune system. After administeringOvalbumin (OVA) with Ojeok-san, Proliferation of Peyer's patch cell was measuredto investigate gut immunostimulatory effect. Results: in vitro cytotoxicity analysis, the inhibitory concentration (IC)50 of thecolon 26-M3.1 carcinoma cell was 890 μg/ml. IC50 of the Peyer's patch cells withLPS was 990 μg/ml. We found that orally administered Ojeok-san significantly inhibitedtumor metastasis in vivo. In addition, the amounts of IL-4 and GM-CSF in the culturesupernatant of Peyer's patch cells were significantly increased compared to the controlgroup. The proliferation of bone marrow cell was significantly up-regulated withOjeok-san. These results indicate that oral administration of Ojeok-san enhances thesecretion of hematopoietic growth factors such as GM-CSF and IL-4 from Peyer's patchcells, and these cytokines also act on modulator of bone marrow cell proliferation. After orally administering Ovalbumin (OVA) with Ojeok-san, IgA induction andProliferation of peyer's patch cell was up-regulated with Ojeok-san. These resultsmeans orally administered Ojeok-san activates intestinal immune system and hasan inhibitory effect on tumor metastasis. Conclusions: Orally administered Ojeok-san appears to have considerable activityon the anti-metastasis by activation of immune system.
Abstract
Objectives: This study was designed to investigate intestinal immune systemactivation and antimetastatic effect of Ojeok-san on cancer cells by oral administration. Methods: Cell viability of Ojeok-san was tested with colon 26-M3.1 carcinomacells and Peyer's patch cells in vitro. Antimetastatic experiments were conductedin vivo mouse model by using colon 26-M3.1 carcinoma cell. To observe immunomodulatingeffects of Ojeok-san on Peyer's patch cells, we measured interleukin (IL)-4, GM-CSF. In addition to observing effects of Ojeok-san on hematopoiesis, we measured proliferationof bone marrow cells mediated by Peyer's patch cells in vitro. IgA induction activated in serum and intestinal content was measured to observethe effect of orally administered Ojeok-san on mucosal immune system. After administeringOvalbumin (OVA) with Ojeok-san, Proliferation of Peyer's patch cell was measuredto investigate gut immunostimulatory effect. Results: in vitro cytotoxicity analysis, the inhibitory concentration (IC)50 of thecolon 26-M3.1 carcinoma cell was 890 μg/ml. IC50 of the Peyer's patch cells withLPS was 990 μg/ml. We found that orally administered Ojeok-san significantly inhibitedtumor metastasis in vivo. In addition, the amounts of IL-4 and GM-CSF in the culturesupernatant of Peyer's patch cells were significantly increased compared to the controlgroup. The proliferation of bone marrow cell was significantly up-regulated withOjeok-san. These results indicate that oral administration of Ojeok-san enhances thesecretion of hematopoietic growth factors such as GM-CSF and IL-4 from Peyer's patchcells, and these cytokines also act on modulator of bone marrow cell proliferation. After orally administering Ovalbumin (OVA) with Ojeok-san, IgA induction andProliferation of peyer's patch cell was up-regulated with Ojeok-san. These resultsmeans orally administered Ojeok-san activates intestinal immune system and hasan inhibitory effect on tumor metastasis. Conclusions: Orally administered Ojeok-san appears to have considerable activityon the anti-metastasis by activation of immune system.
참고문헌 (35)
- 통계청, "2011년 사망원인통계", 통계청, ~ (2011)
- "2008년 국가암등록통계"[URL]
- 이진영, "가열산화홍화유의 돌연변이 원성에 관한 연구", 한국식품영양과학회지, 29(1), pp.120~127 (2000)
- Kadhim S, "Synergistic anti-tumor activity of a novel immunomodulator, BCH -1393, in combination with cyclophosphamide", Int J Immunopharmacol, 22(9), pp.659~671 (2000)
- 한의부인과학교재편찬위원회, "한의부인과학(상)", pp.303~305 (2002), 정담
- 허준, "東醫寶鑑", pp.1379~ (2005), 東醫寶鑑출판사
- 한송이, "홍화가 인체 대장암세포에 미치는 효과", 한국한의학연구원논문집, 17(2), pp.129~134 (2011), 한국한의학연구원
- 이지영, "桔梗이 免疫活性 및 抗癌에 미치는 영향", 대한한방부인과학회지, 23(4), pp.10~19 (2010), 대한한방부인과학회
- 허자경, "免疫活性에 의한 夏枯草의 癌轉移 抑制 效果", 대한한방부인과학회지, 22(1), pp.41~52 (2009), 대한한방부인과학회
- 陳師文, "太平惠民和劑局方", pp.59~ (1999), 遼寧科學技術出版社
- 강순수, "바른 방제학", pp.260~261 (1996), 대성문화사
- 이윤재, "免疫活性化 및 轉移抑制 측면에서 五積散이 癌에 미치는 영향", 박사, 한의학과 부인과학전공, pp.2012
- 전미혜, "五積散이 子宮筋腫細胞의 死滅과 Cell Apoptosis에 미치는 影響", 대한한방부인과학회지, 16(2), pp.45~55 (2003), 대한한방부인과학회
- Lavelle EC, "Mucosal immunogenicity of plant lectins in mice", Immunology, 99(1), pp.30~37 (2000)
- 해리슨내과학 편집위원회, "Harrison's 내과학", pp.514~515 (2003), 도서출판MIP
- 박재우, "중국의 암치료 현황", 대한암한의학회지, 11(1), pp.65~73 (2006)
- Ohnishi Y, "Oral administration of a Kampo (Japanese herbal) medicine Juzen-taiho-to inhibits liver metastasis of colon 26-L5 carcinoma cells", Jpn J Cancer Res, 89(2), pp.206~203 (1998)
- Chino A, "Juzentaihoto, a Kampo medicine, enhances IL-12 production by modulating Toll-like receptor 4 signaling pathways in murine peritoneal exudate macrophages", Int Immunopharmacol, 5(5), pp.871~882 (2005)
- Tsuchiya M, "Protective effect of Juzen-taiho-to on hepatocarcinogenesis is mediated through the inhibition of Kupffer cell-induced oxidative stress", Int J Cancer, 123(11), pp.2503~2511 (2008)
- 郁仁存, "中醫腫瘤學", pp.1~11 (1983), 北京科學出版社
- 정재중, "四物湯加味方의 항암효과에 대한 실험적 연구", 대한한방부인과학회지, 23(3), pp.38~55 (2010), 대한한방부인과학회
- 김종욱, "白頭翁湯의 대장암 세포주 HCT-116 항암효과와 세포자멸사에 관한 연구", 대한한방내과학회지, 31(2), pp.273~289 (2010), 대한한방내과학회
- 황도연, "方藥合編", pp.352~353 (2003), 일월서각
- 金想贊, "方劑學", pp.268~270 (1999), 永林社
- Ha H, "Genotoxicity assessment of a herbal formula, Ojeok-san", J Ethnopharmacol, 135(2), pp.586~589 (2011)
- Ha H, "Evaluation of safety of the herbal formula Ojeok-san: acute and sub-chronic toxicity studies in rats", J Ethnopharmacol, 131(2), pp.410~416 (2010)
- 김재형, "五積散이 흰쥐의 고지혈증에 미치는 영향에 대한 실험적 연구", 대한예방한의학회지, 8(2), pp.185~202 (2004)
- 김종대, "五積散이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF발현에 미치는 영향", 대한한방부인과학회지, 24(1), pp.27~41 (2011), 대한한방부인과학회
- Saito J, "Anticlastogenic effect of magnolol on benzo(a)pyrene-induced clastogenicity in mice", Food Chem Toxicol, 46(2), pp.694~700 (2008)
- Mowat AM, "Anatomical basis of tolerance and immunity to intestinal antigens", Nat Rev Immunol, 3(4), pp.331~341 (2003)
- 미생물 면역 분과학회, "최신면역학", pp.170~ (2011), 라이프사이언스
- Hong T, "Enhanced production of hematopoietic growth factors through T cell activation in Peyer's patches by oral administration of Kampo (Japanese herbal) medicine “Juzen-Taiho-To"", Phytomedicine, 5, pp.353~360 (1998)
- Peter JD, "면역학", pp.72~ (2012), (주)교보문고
- 정재혁, "桑白皮의 선천면역 활성화에 의한 항암 효과", 대한한방부인과학회지, 22(1), pp.31~40 (2009), 대한한방부인과학회
- Lai YH, "Microparticle transport in the human intestinal M cell model", J Drug Target, 16, pp.36~42 (2008)