芷貝散 추출물의 염증억제와 선천면역 활성에 의한 항암 효과
Antimetastatic Effects of Jipae-san by Inflammation Control and Activation of Innate Immune System
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives: This study was designed to investigate the anti-tumor metastasis by anti-inflammatory and innate immunomodulating effects of extracts of Jipae-san on cancer cells. Methods: Antimetastatic experiments were conducted in vivo mouse model by using 4T1 mouse mammary carcinoma cells. Cell viability of Jipae-san was tested with 4T1 mouse mammary carcinoma cells, colon 26-M3.1 carcinoma cells and macrophage. In addition expression of TNF-α and NO induced by LPS was measured after treating with Jipae-san. To observe innate immunomodulating effects of Jipae-san on macrophage, we measured TNF-α, IL-12, IL-6 and MCP-1, respectively. Cell cytotoxicity was tested with the macrophage stimulated with Jipae-san and we evaluated the activation of TNF-α and NO. And the effect of Jipae-san on metastasis was measured without NK-cell using GM1 serum. Results: Intravenous inoculation of Jipae-san significantly inhibited metastasis of 4T1 mouse mammary carcinoma cells. In an in vitro cytotoxicity analysis, cell growth are closer to 100% less than 1,000 μg/ml concentration. The expression of TNF-α and NO induced by LPS after treating Jipae-san was down regulated in dose-dependent manner. Level of cytokines such as TNF-α, IL-12, IL-6 and MCP-1 of Jipae-san group were up regulated in compared to the control group. The macrophage stimulated with Jipae-san significantly inhibits the cancer cell at ratio of 10:1, 20:1. The activation of NO was significantly up regualted in a group of 5:1, 10:1, 20:1. The depletion of NK-cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Jipae-san on tumor metastasis. Conclusions: Jipae-san appears to have considerable activity on the anti-metastasis by inflammation control and activation of innate immune system.
Abstract
Objectives: This study was designed to investigate the anti-tumor metastasis by anti-inflammatory and innate immunomodulating effects of extracts of Jipae-san on cancer cells. Methods: Antimetastatic experiments were conducted in vivo mouse model by using 4T1 mouse mammary carcinoma cells. Cell viability of Jipae-san was tested with 4T1 mouse mammary carcinoma cells, colon 26-M3.1 carcinoma cells and macrophage. In addition expression of TNF-α and NO induced by LPS was measured after treating with Jipae-san. To observe innate immunomodulating effects of Jipae-san on macrophage, we measured TNF-α, IL-12, IL-6 and MCP-1, respectively. Cell cytotoxicity was tested with the macrophage stimulated with Jipae-san and we evaluated the activation of TNF-α and NO. And the effect of Jipae-san on metastasis was measured without NK-cell using GM1 serum. Results: Intravenous inoculation of Jipae-san significantly inhibited metastasis of 4T1 mouse mammary carcinoma cells. In an in vitro cytotoxicity analysis, cell growth are closer to 100% less than 1,000 μg/ml concentration. The expression of TNF-α and NO induced by LPS after treating Jipae-san was down regulated in dose-dependent manner. Level of cytokines such as TNF-α, IL-12, IL-6 and MCP-1 of Jipae-san group were up regulated in compared to the control group. The macrophage stimulated with Jipae-san significantly inhibits the cancer cell at ratio of 10:1, 20:1. The activation of NO was significantly up regualted in a group of 5:1, 10:1, 20:1. The depletion of NK-cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Jipae-san on tumor metastasis. Conclusions: Jipae-san appears to have considerable activity on the anti-metastasis by inflammation control and activation of innate immune system.
참고문헌 (38)
- "통계청"[URL]
- 김종한, "국내 한의학 암치료 임상연구 논문에 대한 고찰", 대한침구의학회지, 29(3), pp.19~28 (2012), 대한침구의학회[DOI]
- 함영안, "잔대 추출물들의 항돌연변이 및 항종양 효과", 한국식품영양과학회지, 38(1), pp.25~31 (2009), 한국식품영양과학회
- Scott E, "Strategies for Antigen Loading of Dendritic Cells to Enhance the Antitumor Immune Response", Cancer Research, 62, pp.1884~1889 (2002)
- Ha ES, "Anti-metastatic activity of glycoprotein fractionated from Acanthpanax senticosus, involvement of NK-cell and macrophage activation", Arch Pharm Res, 27(2), pp.217~224 (2004)
- 조종관, "한방임상종양학", pp.3~12 (2001), 주민출판사
- 최승훈, "東醫腫瘍學", pp.78~81 (1995), 행림출판
- 윤성우, "乾漆 추출물(Nexia)의 독성 실험과 급성 림프구성 백혈병환자의 임상증례 군 보고", 대한암한의학회지, 11(1), pp.1~21 (2006)
- 하태현, "산삼약침과 FOLFIRI 항암화학요법의 병행으로 호전된 전이성 대장암 환자 1례", 동의생리병리학회지, 27(1), pp.148~151 (2013), 대한동의병리학회
- 조유경, "울금이 위암세포에 미치는 영향", 대한한방종양학회지, 9(1), pp.15~37 (2003)
- 김흥수, "유근피의 선천 면역 활성화에 의한 암 전이 억제 효과", 대한한방부인과학회지, 23(1), pp.1~11 (2010), 대한한방부인과학회
- 이윤재, "免疫活性化 및 轉移抑制 측면에서 五積散이 癌에 미치는 영향", 박사, 한의학과 부인과학전공, pp.2012
- 李梴, "新對譯編註 醫學入門", pp.2004~ (2009), 法仁文化社
- 黃度淵, "方藥合編", pp.243~244 (1993), 여강출판사
- 허준, "東醫寶鑑", pp.685~686 (2005), 동의보감출판사
- 이상현, "芷貝散의 抗炎症 效能에 대한 硏究", 대한한의학 방제학회지, 16(1), pp.79~94 (2008), 대한한의학방제학회
- 황기명, "인간백혈병 세포주에서 지패산의 작용에 관한 연구", 대한한의학 방제학회지, 10(1), pp.143~155 (2002)
- Kipanyula MJ, "Signaling pathways bridging microbial-triggered inflammation and cancer", Cell Signal, 25(2), pp.403~416 (2013)
- Trinchieri G, "Interleukin-12: A proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity", Annu Rev Immunol, 13, pp.251~276 (1995)
- Blom B, "Development of human lymphoid cells", Annu Rev Immunol, 24, pp.287~320 (2006)
- 이영찬, "파두를 가미한 사군자탕 및 사물탕의 항암효과에 대한 연구", 동의생리병리학회지, 9(1), pp.79~100 (1994)
- 權承鳳, "本草學", pp.130~ (2000), 永林社
- Ban HS, "Inhibitory Effects of Furanocoumarins Isolated from the Roots of Angelica dahurica on Prostaglandin E2 Production", Planta Med, 69, pp.408~412 (2003)
- Kang SW, "Rapid identification of furanocoumarins in Angelica dahurica using the Online LC-MMR-MS and their nitirc oxide inhibitory activity in RAW 264.7 cells", Phytochemical Analysis, 21(4), pp.322~327 (2010)
- Pae HO, "Differentiation-inducing effects of verticinone, an isosteroidal alkaloid isolated from the bulbus of Fritillaria Verticillata, on human promyelocytic leukemia HL-60 cells", Biol Pharm Bull, 25(11), pp.1409~1411 (2002)
- Kang DG, "Effects of bulb of Fritillaria Ussuriensis maxim. on angiotensin converting enzyme and vascular release of NO", cGMP in rats, 2002, pp.81(1), 49~55
- Gee K, "Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS- induced CD44 expression", J Immunol, 169, pp.5660~5672 (2002)
- Guha M, "LPS induction of gene expression in human monocytes", Cell Signal, 13, pp.85~94 (2001)
- Paik YH, "Toll-like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells", Hepatology, 37, pp.979~982 (2003)
- Chino A, "Juzentaihoto, a kampo medicine, enhances IL-12 production by modulating toll-like receptor 4 signaling pathways in murine peritoneal exudate macrophages", Int Immunopharmacol, 5, pp.871~882 (2005)
- Ha ES, "Anti-metastatic Activity of Glycoprotein Fractionated from Acanthopanax senticosus, Involvement of NK-cell and Macrophage Activation", Arch Pharm Res, 27(2), pp.217~224 (2004)
- Shin FN, "A balance of interleukin-12 and -23 in cancer", Trends in immunology, 34(11), pp.548~555 (2013)
- 대한진단검사의학회, "진단검사의학", pp.628~ (2001), 고려의학
- Kishimoto T, "The biology of interleukin-6", Blood, 74(1), pp.1~10 (1989)
- Kurebayashi J, "Medroxyprogesterone acetate inhibits interleukin 6 secretion from KPL-4 human breast cancer cells both in vitro and in vivo: a possible mechanism of the anticachectic effect", Br J Cancer, 79(3, pp.4) (1999), 631~636
- Mantovani A, "The origin and function of tumor-associated macrophages", Immunol Today, 13(7), pp.265~270 (1992)
- RJ Mackay, "Protein changes associated with stages of activation of mouse macrophages for tumor cell killing", J. Immunol, 137, pp.1392~1398 (1986)
- 서울대학교의과대학, "종양학", pp.229~ (1998), 서울대학교출판부