본문 바로가기
KERD
논문KCI 등재

Expression and characterization of RNA-dependent RNA polymerase of Ectropis obliqua virus

Meijuan Lin|Shan Ye|Yi Xiong|Dawei Cai|Jiamin Zhang|Yuanyang Hu
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2010.04|BMB Reports|제43권 제4호|pp. 284-290

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

Ectropis obliqua virusIflavirusPrimer-dependentRNAdependent RNA polymeraseRNA synthesis
주제분류화학

초록

Replication of positive-strand RNA virus is mediated by a virus-encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Ectropis obliqua virus (EoV), a newly identified insect virus belonging to the family Iflaviradae, we expressed the RNA polymerase domain in Escherichia coli and purified it on a Ni-chelating HisTrap affinity column. It is demonstrated that EoV RdRp initiated RNA synthesis in a primerand poly (A)-dependent manner in vitro. Furthermore, the effect of primer concentration, temperature, metal ions (Mg2+,Mn2+, and K+) on enzymatic activity were determined. Our study represented a first step towards understanding the mechanism of EoV replication.

Abstract

Replication of positive-strand RNA virus is mediated by a virus-encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Ectropis obliqua virus (EoV), a newly identified insect virus belonging to the family Iflaviradae, we expressed the RNA polymerase domain in Escherichia coli and purified it on a Ni-chelating HisTrap affinity column. It is demonstrated that EoV RdRp initiated RNA synthesis in a primerand poly (A)-dependent manner in vitro. Furthermore, the effect of primer concentration, temperature, metal ions (Mg2+,Mn2+, and K+) on enzymatic activity were determined. Our study represented a first step towards understanding the mechanism of EoV replication.

참고문헌 (28)

  1. Wang, X., "Sequence analysis and genomic organization of a new insect picorna-like virus, Ectropis obliqua picornalike virus, isolated from Ectropis obliqua", J. Gen. Virol., 85, pp.1145~1151 (2004)
  2. Isawa, H., "Analysis of genetic information of an insect picorna-like virus, infectious flacherie virus of silkworm: evidence for evolutionary relationships among insect, mammalian and plant picorna(-like) viruses", Arch. Virol., 143, pp.127~143 (1998)
  3. Wu, C. Y., "The complete sequence of Perina nuda picorna- like virus, an insect infecting RNA virus with a genome organization similar to that of the mammalian picornaviruses", Virology, 294, pp.312~323 (2002)
  4. Ghosh, R. C., "The nucleotide sequence of sacbrood virus of the honey bee: an insect picorna-like virus", J. Gen. Virol., 80, pp.1541~1549 (1999)
  5. Ongus, J. R., "Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor", J. Gen. Virol., 85, pp.3747~3755 (2004)
  6. Lanzi, G., "Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.)", J. Virol., 80, pp.4998~5009 (2006)
  7. Christian, P., "Iflavirus; in Virus Taxonomy: Eighth Report of the International Committee on the Taxonomy of Viruses, Fauquet", pp.779~782 (2005), Academic Press
  8. Kok, C. C., "Picornavirus RNAdependent RNA polymerase", Int. J. Biochem. Cell B., 41, pp.498~502 (2009)
  9. Paul, A.V., "Protein-primed RNA synthesis by purified poliovirus RNA polymerase", Nature, 393, pp.280~284 (1998)
  10. Zhou, L., "Expression and characterization of RNA-dependent RNA polymerase of Dendrolimus punctatus tetravirus", J. Biochem. Mol. Biol., 39, pp.571~577 (2006)
  11. Lohmann V., "Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity", J. Virol., 71, pp.8416~8428 (1997)
  12. Ishii K., "Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding", Hepatology, 29, pp.227~1235 (2003)
  13. Choi, K. H., "The structure of the RNA-dependent RNA polymerase from bovine viral diarrhea viruse stablishes the role of GTP in de novo initiation", Proc. Natl. Acad. Sci. U.S.A., 통권 : 101, pp.4425~4430 (2004)
  14. Xiao, M., "Characterization of RNA-dependent RNA Polymerase Activity of CSFV NS5B proteins Expressed in Escherichia coli", Virus Genes, 27, pp.67~74 (2003)
  15. Koonin, E. V., "The phylogeny of RNA-dependent polymerase of positive-strand RNA viruses", J. Gen. Virol., 72, pp.2197~2206 (1991)
  16. Sankar, S., "Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase", J. Virol., 65, pp.2993~3000 (1991)
  17. Neufeld, K. L., "Identification of terminal adenylyl transferase activity of the poliovirus 3Dpol", J. Virol., 68, pp.5811~5818 (1994)
  18. Ranjith-Kumar, C. T., "Terminal nucleotidyl transferase activity of recombinant Flaviviridae RNA-dependent RNA polymerases: implication for viral RNA synthesis", J. Virol., 75, pp.8615~8623 (2001)
  19. Guan, H. C., "Polymerization of nontemplate bases before transcription initiation at the 3' ends of templates by an RNA-dependent RNA polymerase: An activity involved in 3' end repair of viral RNAs", Proc. Natl. Acad. Sci. U.S.A., 통권 : 97, pp.12451~12456 (2000)
  20. Tomei. L., "Biochemical characterization of a hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence", J. Gen. Virol., 81, pp.759~767 (2000)
  21. Yi, G., "De novo RNA synthesis by a recombinant classical swine fever virus RNAdependent RNA polymerase", Eur. J. Bioehem., 270, pp.4952~4961 (2003)
  22. Hey, T. D., "Synthesis of Plus- and Minus-Strand RNA from Poliovirion RNA template in vitro", J. Virol., 58, pp.790~796 (1986)
  23. Lubinski, J. M., "Primer-dependent synthesis of covalently linked dimeric RNA molecules by poliovirus replicase", J. Virol., 61, pp.2997~3003 (1987)
  24. Yocupicio-Monroy, R. M., "Cellular proteins from human monocytes bind to dengue 4 virus minus-strand 3'untranslated region RNA", J. Virol., 77, pp.3067~3076 (2003)
  25. Piccininni, S., "Modulation of the hepatitis C virus RNA-dependent RNA polymerase activity by the non-structural (NS)3 helicase and the NS4B mem brane protein", J. Biol. Chem., 277, pp.45670~45679 (2002)
  26. Ackermann, M., "De novo synthesis of RNA by the Dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase", J. Biol. Chem., 276, pp.39926~39937 (2001)
  27. Behrens, S. E., "Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus", EMBO J., 15, pp.12~22 (1996)
  28. Crotty, S., "Manganese-dependent polioviruses caused by mutations within the viral polymerase", J. Virol., 77, pp.5378~5388 (2003)

관련 논문AI추천

Characterization of the Open Reading Frame 35 of Bombyx mori NucleopolyhedrovirusGuo Hui Li, Ke Ping Chen, Qin Yao, Ying Min Zhu, Zhong Jian Guo한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2010 · 제21권 제2호Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence김도영, 김휘배, 조태주생화학분자생물학회 · BMB Reports2014 · 제47권 제6호Genome Sequences of Spinach Deltapartitivirus 1, Spinach Amalgavirus 1, and Spinach Latent Virus Identified in Spinach Transcriptome박동빈, 한윤수한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제7호Characterization of Echinostoma cinetorchis endoribonuclease, RNase H김동수, 박서혜, 박영일, 박장호, 이형우, 임성빈, 전보영, 전호정, 제갈승, 차석호대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2017 · 제55권 제4호A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase정웅, 안희철, 이진무, 정용주, 조진범한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제11호Dependence of RIG-I Nucleic Acid-Binding and ATP Hydrolysis on Activation of Type I Interferon Response김동은, 백유미, 윤수진, 황여은대한면역학회 · Immune Network2016 · 제16권 제4호

이 간행물의 다른 논문 (11건)

The role of neuroinflammation on the pathogenesis of Parkinson’s disease허수희, 정영철, 복유진, 진병관, 남진한, 고혁완pp. 225-232Biochemical and molecular features of LRRK2 and its pathophysiological roles in Parkinson’s disease설원기pp. 233-244Inhibitory effects of KHG26377 on glutamate dehydrogenase activity in cultured islets조성우, 최수영, 양승주, 한호규pp. 245-249Enhancement of immunomodulatory activity by liposome-encapsulated natural phosphodiester bond CpG-DNA in a human B cell line이영희, 김영은, 최수영, 박진서, 권상훈, 권형주, 김동범, Jae Won Rheepp. 250-256Influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in Sus ScrofaPavel Martasek, Oxana V. Klimenko, Vaclav Vobrubapp. 257-262
원문 보기