Modification of Turnip yellow mosaic virus coat protein and its effect on virion assembly
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Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus. We have modified TYMV coat protein (CP) by inserting a c-Myc epitope peptide at the N- or C-terminus of the CP, and have examined its effect on assembly. We introduced the recombinant CP constructs into Nicotiana benthamiana leaves by agroinfiltration. Examination of the leaf extracts by agarose gel electrophoresis and Western blot analysis showed that the CP modified at the N-terminus produced a band co-migrating with wild-type virions. With C-terminal modification, however,the detected bands moved faster than the wild-type virions. To further examine the effect, TYMV constructs producing the modified CPs were prepared. With N-terminal modification,viral RNAs were protected from RNase A. In contrast, the viral RNAs were not protected with C-terminal modification. Overall, the results suggest that virion assembly and RNA packaging occur properly when the N-terminus of CP is modified, but not when the C-terminus is modified. [BMB Reports 2013; 46(10): 495-500]
Abstract
Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus. We have modified TYMV coat protein (CP) by inserting a c-Myc epitope peptide at the N- or C-terminus of the CP, and have examined its effect on assembly. We introduced the recombinant CP constructs into Nicotiana benthamiana leaves by agroinfiltration. Examination of the leaf extracts by agarose gel electrophoresis and Western blot analysis showed that the CP modified at the N-terminus produced a band co-migrating with wild-type virions. With C-terminal modification, however,the detected bands moved faster than the wild-type virions. To further examine the effect, TYMV constructs producing the modified CPs were prepared. With N-terminal modification,viral RNAs were protected from RNase A. In contrast, the viral RNAs were not protected with C-terminal modification. Overall, the results suggest that virion assembly and RNA packaging occur properly when the N-terminus of CP is modified, but not when the C-terminus is modified. [BMB Reports 2013; 46(10): 495-500]
참고문헌 (29)
- Dreher, T. W., "Turnip yellow mosaic virus: transfer RNA mimicry, chloroplasts and a C-rich genome", Mol. Plant Pathol, 5, pp.367~375 (2004)
- Finch, J. T, "Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus: II. Electron microscopic studies", J. Mol. Biol, 15, pp.344~364 (1966)
- Böttcher, B, "Difference imaging reveals ordered regions of RNA in turnip yellow mosaic virus", Structure, 4, pp.387~394 (1996)
- Canady, M. A., "Crystal structure of turnip yellow mosaic virus", Nat. Struct. Biol, 3, pp.771~781 (1996)
- Van Loon, A. -M. M., "Crystal structure of an empty capsid of Turnip yellow mosaic virus. J. Mol. Biol", J. Mol. Biol, 341, pp.1205~1214 (2004)
- Morgan, G. J, "Historical review: Viruses, crystals and geodesic domes", Trends Biochem. Sci, 28, pp.86~90 (2003)
- Hirth, L., "Tymoviruses; in The Plant Viruses", pp.163~212 (1985), Plenum Press
- Steinmetz, N. F., "Utilization of plant viruses in biotechnology", Org. Biomol. Chem, 5, pp.2891~2902 (2007)
- Ren, Y., "Application of plant viruses as nano drug delivery systems", Pharm. Res, 27, pp.2509~2513 (2010)
- Gleba, Y., "Viral vectors for the expression of proteins in plants", Curr. Opin. Biotech, 18, pp.134~141 (2007)
- Noad, R., "Virus-like particles as immunogens", Trends Microbiol, 11, pp.438~444 (2003)
- Lewis, J. D., "Viral nanoparticles as tools for intravital vascular imaging", Nature Med, 12, pp.354~360 (2006)
- Prasuhn, D. E., "Viral MRI contrast agents: coordination of Gd by native virions and attachment of Gd complexes by azide-alkyne cycloaddition", Chem. Commun, 2007, pp.1269~1271 (2007)
- Lockney, D. M., "The Red clover necrotic mosaic virus capsid as a multifunctional cell targeting plant viral nanoparticle", Bioconjugate Chem, 22, pp.67~73 (2011)
- Ren, Y., "Folic acid-conjugated protein cages of a plant virus: A novel delivery platform for doxorubicin", Bioconjugate Chem, 18, pp.836~843 (2007)
- Barnhill, H. N., "Turnip yellow mosaic virus as a chemoaddressable bionanoparticles", Bioconjugate Chem, 18, pp.852~859 (2007)
- Zeng, Q., "Chemoselective modification of Turnip yellow mosaic virus by Cu(I) catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction and its application in cell binding", Bioconjugate Chem, 22, pp.58~66 (2011)
- Hayden, C. M., "Turnip yellow mosaic virus isolates with experimentally produced recombinant virion proteins", J. Gen. Virol, 79, pp.395~403 (1998)
- Sastri, M., "Identification of a discrete intermediate in the assembly", disassembly of physalis mottle tymovirus through mutational analysis, 1999, pp.289, 905~918
- Bransom, K. L., "Coding density of the turnip yellow mosaic virus genome : roles of the overlapping coat protein and p206-readthrough coding regions", Virology, 206, pp.403~412 (1995)
- Cho, T.-J., "Encapsidation of genomic but not subgenomic Turnip yellow mosaic virus RNAS by coat protein provided in trans", Virology, 356, pp.126~135 (2006)
- Matsuda, D., "The tRNA-like structure of Turnip yellow mosaic virus RNA is a 3'-translational enhancer", Virology, 321, pp.36~46 (2004)
- Hyun-Il Shin, "A Sequence in Coat Protein Open Reading Frame Is Required for Turnip Yellow Mosaic Virus Replication", Journal of Bacteriology and Virology, 41(2), pp.109~116 (2011), 대한미생물학회
- 신현일, "Characterization of a Replication Element in the Coat Protein ORF of Turnip Yellow Mosaic Virus", Journal of Bacteriology and Virology, 42(1), pp.49~55 (2012), 대한미생물학회
- Shin, H. -I., "The 5’-UTR of Turnip yellow mosaic virus does not include a critical encapsidation signal", Virology, 387, pp.427~435 (2009)
- Quesniaux, V., "Immunochemical studies of turnip yellow mosaic virus-II. Localization of a viral epitope in the N-terminal residues of the coat protein", Molec. Immun, 20, pp.179~185 (1983)
- Quesniaux, V., "Immunochemical studies of turnip yellow mosaic virus-III. Localization of two viral epitopes in residues 57-64 and 183-189 of the coat protein", Bioch. Biophys. Acta, 743, pp.226~231 (1983)
- Hyun-Il Shin, "Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA", BMB reports, 41(10), pp.739~744 (2008), 한국생화학분자생물학회
- 이선화, "Transduced Tat-Annexin protein suppresses inflammationassociated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells", BMB Reports, 44(7), pp.484~489 (2011), 생화학분자생물학회