초음파치료가 종양세포에 미치는 효과

Abstract

The use of therapeutic ultrasound(US) in humans with malignant neoplasms has beencontraindicated in physical therapy practice. Some studies have shown that results afterapplication of US differ according to tumor type and penetratjon depth. The purposes ofthis study were to determine the effects of US on melanoma in mice and to determinetreatment dosage. Twenty-four female C57BL/6 mice, age 8 weeks. The right flank of 떠lmice was shaved, and a 0.1 ml suspension of cells was injected subcutaneously into thea띠mals ’ right flank. In this study, 24 subjects were randomly divided into three groups:experimental group 1<n=8), experimental group 2(n=8), control group(n=8). In theexperil)1ental group 1, animals received continuous 3 MHZ US treatment, administered at 2.0W!cm‘ for five minutes. In experime.ntal group 2, animals received continuous 3 MHz UStreatment, administered at 1.0 W!cm" for 5 minutes. The control group received the samehandling as other experimental groups, including rodent chow, water, US gel application but US head pressure without the power turned on. After 10 days treatment, all mice werekilled with a potassium solution. Tumors were excised and weighed on an electrical balanceand fixed in a 10% neutral buffered formalin solution. Tumor weights were smaller inexperimental group 2<0.3838 g) than in the control group<O.6275 g). Tumor weights of theexperimental group 1<0.015 g) were smaller than those of experimental group 2. Continuoustherapeutic US decreased the weight of subcutaneous melanoma tumors in mice. Thetreatment dosage(2.0 W/cm2) we suggest was more effective than earlier studies ondecreasing tumor size with ultrasound.