Identification of Polymorphisms of Fas Gene and Association Analysis in Hanwoo
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Fas gene known to associate with intramuscular fat content in Korean cattle was selected for DNA marker development. Fas (APO-1, CD95), a member of the tumor necrosis factor(TNF) receptor superfamily, is a cell membrane protein that mediates apoptosis(programmed cell death). We discovered single nucleotide polymorphisms(SNPs) within Fas gene in order to develop novel DNA markers at genomic level. Of this gene to search for SNP, sequences of whole exon and 1kb range of both front and back of the gene using 24 cattle were determined by direct-sequencing methods. As a result, 16 SNPs in exon, 37 SNPs in intron and 2 SNPs in promoter region, a total of 55 SNPs were discovered. In these SNPs, thirty-one common polymorphic sites were selected considering their allele frequencies, haplotype-tagging status and Linkage Disequilibrium(LD) for genotyping in larger-scale subjects. Selected SNPs were confirmed genotype through SNaPshot method(n=274) and were examined for possible genetic association of Fas polymorphisms with carcass weight(CWT), eye muscle area(EMA), and backfat thickness(BF). So, the SNP have been identified significant g.-12T>G, g.1112T>G and g.32548T>C. These results suggest that polymorphism of Fas gene was associated with meat quality traits in Hanwoo.
Abstract
Fas gene known to associate with intramuscular fat content in Korean cattle was selected for DNA marker development. Fas (APO-1, CD95), a member of the tumor necrosis factor(TNF) receptor superfamily, is a cell membrane protein that mediates apoptosis(programmed cell death). We discovered single nucleotide polymorphisms(SNPs) within Fas gene in order to develop novel DNA markers at genomic level. Of this gene to search for SNP, sequences of whole exon and 1kb range of both front and back of the gene using 24 cattle were determined by direct-sequencing methods. As a result, 16 SNPs in exon, 37 SNPs in intron and 2 SNPs in promoter region, a total of 55 SNPs were discovered. In these SNPs, thirty-one common polymorphic sites were selected considering their allele frequencies, haplotype-tagging status and Linkage Disequilibrium(LD) for genotyping in larger-scale subjects. Selected SNPs were confirmed genotype through SNaPshot method(n=274) and were examined for possible genetic association of Fas polymorphisms with carcass weight(CWT), eye muscle area(EMA), and backfat thickness(BF). So, the SNP have been identified significant g.-12T>G, g.1112T>G and g.32548T>C. These results suggest that polymorphism of Fas gene was associated with meat quality traits in Hanwoo.
참고문헌 (21)
- Report of business for animal products grading, "APGS", Animal products grading system, National Co-operatives Feder- ation, Korea, ~ (1995)
- Camerini, D, "The T cell activation antigen CD27 is a member of the nerve growth factor", tumor necrosis factor receptor gene family, 1991, pp.147, 3165~3169
- Casas, E, "Identification of quantitative trait loci for growth and carcass composition in cattle", Anim. Genet, 35, pp.2~6 (2004)
- Darvasi, A, "Selective DNA pooling for determination of linkage between a molecular marker and a quantitative trait locus", Genetics, 138, pp.1365~1373 (1994)
- Gilmour, A. R, "ASREML User Guide Release 2.0" (2006), VSN International Ltd., Hempstead, HP1 1ES, UK
- Itoh, N, "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis", Cell, 66, pp.233~243 (1991)
- Johnson, D, "Expression and structure of the human NGF receptor", Cell, 47, pp.545~554 (1986)
- Kim, N. K, "Heat shock protein b1 and its regulator genes are negatively correlated with intramuscular fat content in the longissimus thoracis muscle of hanwoo(korean cattle) steers", J. Agric. Food Chem, 59, pp.5657~5664 (2011)
- Lande, R, "Efficiency of Marker-Assisted Selection in the Improvement of Quantitative Traits", Genetics, 124, pp.743~756 (1990)
- Loetscher, H, "Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor", Cell, 61, pp.351~359 (1990)
- Mallett, S, "Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor", EMBO J, 9, pp.1063~1068 (1990)
- Marques, E, "Polymorphisms in positional candidate genes on BTA14 and BTA26 affect carcass quality in beef cattle", J. Anim. Sci, 87, pp.2475~2484 (2009)
- Miller, S. A, "A simple salting out procedure for extracting DNA from human nucleated cells", Nucleic Acids Res, 16, pp.1215~ (1988)
- Nott, A, "A quantitative analysis of intron effects on mammalian gene expression", RNA, 9, pp.607~617 (2003)
- Oehm, A, "Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor", nerve growth factor receptor superfamily. Sequence identity with the Fas antigen, 1992, pp.267, 10709~10715
- Schall, T. J, "Molecular cloning and expression of a receptor for human tumor necrosis factor", Cell, 61, pp.361~370 (1990)
- Stamenkovic, I, "A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas", EMBO J, 8, pp.1403~1410 (1989)
- Stylianou, I, "Quantitative trait locus analysis for obesity reveals multiple networks of interacting loci", Mamm. Genome, 17, pp.22~36 (2006)
- Trauth, B. C, "Monoclonal antibody-mediated tumor regression by induction of apoptosis", Science, 245, pp.301~305 (1989)
- Watanabe-Fukunaga, R, "The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen", J Immunol, 148, pp.1274~1279 (1992)
- Yonehara, S, "A cell-killing monoclonal antibody(anti-Fas) to a cell surface antigen co- downregulated with the receptor of tumor necrosis factor", J. Exp. Med, 169, pp.1747~1756 (1989)