Association of Microsatellite Marker in FABP4 Gene with Marbling Score and Live Weight in Hanwoo
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The bovine fatty acid binding protein 4(FABP4) plays an important role to uptake intracellular fatty acid. It has been previously reported as a positional candidate gene for marbling score in livestock. The re-sequencing of FABP4 gene detected a polymorphic AT repeated sequence in intron II of FABP4 gene. Allelic distribution for this microsatellite marker was examined in other cattle breeds. A total of 8 alleles were detected with diverse repeat units(14 to 21 AT repeat) in Hanwoo and 7 breeds. Of the 8 alleles, the predominant alleles were [AT]16, [AT]18 and [AT]19 in the Hanwoo and 7 cattle breeds. The linear mixed model for genotypic effect (3237AT) on carcass traits showed a significant effect on marbling score(MAR P=0.025) and live weight (LWT; P=0.04) in the 583 Hanwoo cattle population. Live weight(LW) was highest in the homozygous(AT)17 genotype(557.5± 6.94) and lowest in the heterozygous (AT)16/17 genotype(521.7±7.70). On the other hand, the homozygous (AT)17 genotype(3.0± 0.15) has the highest effect on marbling score and the lowest effect was in homozygous(AT)18 genotype(2.2±0.15). The marbling score difference between both groups was 0.8 which is around two times higher than SNP genotype effect on marbling score in Limousin×Wagyu crosses.
Abstract
The bovine fatty acid binding protein 4(FABP4) plays an important role to uptake intracellular fatty acid. It has been previously reported as a positional candidate gene for marbling score in livestock. The re-sequencing of FABP4 gene detected a polymorphic AT repeated sequence in intron II of FABP4 gene. Allelic distribution for this microsatellite marker was examined in other cattle breeds. A total of 8 alleles were detected with diverse repeat units(14 to 21 AT repeat) in Hanwoo and 7 breeds. Of the 8 alleles, the predominant alleles were [AT]16, [AT]18 and [AT]19 in the Hanwoo and 7 cattle breeds. The linear mixed model for genotypic effect (3237AT) on carcass traits showed a significant effect on marbling score(MAR P=0.025) and live weight (LWT; P=0.04) in the 583 Hanwoo cattle population. Live weight(LW) was highest in the homozygous(AT)17 genotype(557.5± 6.94) and lowest in the heterozygous (AT)16/17 genotype(521.7±7.70). On the other hand, the homozygous (AT)17 genotype(3.0± 0.15) has the highest effect on marbling score and the lowest effect was in homozygous(AT)18 genotype(2.2±0.15). The marbling score difference between both groups was 0.8 which is around two times higher than SNP genotype effect on marbling score in Limousin×Wagyu crosses.
참고문헌 (15)
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