Length polymorphism in OGT between Korean native pig, Chinese Meishan, and the Western pig breeds
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Background: The Korean native pig (KNP) is generally thought to have come from northern China to the Koreanpeninsula approximately 2000 years ago. KNP pigs were at the brink of extinction in the 1980s, since then effortshave been made to restore the breed by bringing together the remaining stocks in South Korea. As a result, KNPwas registered as a breed in 2006. To find additional breed-specific markers that are distinct among pig breeds,variations in O-linked N-acetylglucosamine transferase (OGT) were investigated. OGT is located on chromosome X andcatalyzes the post-translational addition of a single O-linked-β-N-acetylglucosamine to target proteins. Findings: Length polymorphism in the intron 20 of OGT was identified. The intron 20 of OGT from Duroc, Landrace,and Yorkshire breeds was 281-bp longer than that from either KNP or Chinese Meishan pigs. The differencebetween the Western pig breeds (BB genotype) and KNP or Meishan pigs (AA genotype) was due to an inserted276-bp element and the 5-bp ACTTG. Conclusions: The polymorphism in OGT identified in this study may be used as an additional marker fordetermining the breed of origin among Meishan and the Western pig breeds. The length polymorphism suggeststhat the locus near OGT is not fixed in KNP. This marker would be relevant in determining the breed of origin incrossbred pigs between KNP pigs with known genotypes and the Western pig breeds with BB genotypes, thusconfirming the contribution of the X chromosome from each breed.
Abstract
Background: The Korean native pig (KNP) is generally thought to have come from northern China to the Koreanpeninsula approximately 2000 years ago. KNP pigs were at the brink of extinction in the 1980s, since then effortshave been made to restore the breed by bringing together the remaining stocks in South Korea. As a result, KNPwas registered as a breed in 2006. To find additional breed-specific markers that are distinct among pig breeds,variations in O-linked N-acetylglucosamine transferase (OGT) were investigated. OGT is located on chromosome X andcatalyzes the post-translational addition of a single O-linked-β-N-acetylglucosamine to target proteins. Findings: Length polymorphism in the intron 20 of OGT was identified. The intron 20 of OGT from Duroc, Landrace,and Yorkshire breeds was 281-bp longer than that from either KNP or Chinese Meishan pigs. The differencebetween the Western pig breeds (BB genotype) and KNP or Meishan pigs (AA genotype) was due to an inserted276-bp element and the 5-bp ACTTG. Conclusions: The polymorphism in OGT identified in this study may be used as an additional marker fordetermining the breed of origin among Meishan and the Western pig breeds. The length polymorphism suggeststhat the locus near OGT is not fixed in KNP. This marker would be relevant in determining the breed of origin incrossbred pigs between KNP pigs with known genotypes and the Western pig breeds with BB genotypes, thusconfirming the contribution of the X chromosome from each breed.
참고문헌 (13)
- KyungSeokKim, "Gentic Structure of Korean Native Pig Using Microsatellite Markers", Genes & Genomics, 24(1), pp.1~7 (2002), 한국유전학회
- Kwon OS, "Preservation and current status of Korean native pig-in Korean", Symposium on preservation and utilization of Korean native pig-in Korean. Chonan, Republic of Korea, pp.3~20 (2006)
- Kim MJ, "Restoration of Korean native pig-in Korean", Raising Korean native pig-in Korean. Chonan, Republic of Korea, pp.21~30 (2008)
- Kim TH, "Genetic structure of pig breeds from Korea and China using microsatellite loci analysis", J Anim Sci, 83, pp.2255~2263 (2005)
- 김명직, "Microsatellite Marker를 이용한 한국재래돼지 집단의 품종특성 및 원산지 추적을 위한 개체식별체계 설정", 한국축산식품학회지, 27(2), pp.150~156 (2007), 한국축산식품학회
- Butkinaree C, "O-linked beta-N-acetylglucosamine(O-GlcNAc) : Extensive crosstalk with phosphorylation to regulate signaling and transcription in response to nutrients and stress", Biochim Biophys Acta, 2010, pp.96~106 (1800)
- Hanover JA, "The hexosamine signaling pathway : O-GlcNAc cycling in feast or famine", Biochim Biophys Acta, 2010, pp.80~95 (1800)
- Kim JG, "Molecular cloning of porcine OGT cDNA and mapping to the X chromosome", Biol Reprod, (Supplement), pp.154~ (2006)
- Rohrer GA, "Identification of genomic regions controlling plasma FSH concentrations in Meishan-White Composite boars", Physiol Genomics, 6, pp.145~151 (2001)
- Ford JJ, "Interrelationships of porcine X and Y chromosomes with pituitary gonadotropins and testicular size", Biol Reprod, 65, pp.906~912 (2001)
- E.H. Kim, "Detection of Mendelian and Parent-of-origin Quantitative Trait Loci in a Cross between Korean Native Pig and Landrace I. Growth and Body Composition Traits", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 20(5), pp.669~676 (2007), 아세아·태평양축산학회
- S.W. Kim, "QTL Scan for Meat Quality Traits Using High-density SNP Chip Analysis in Cross between Korean Native Pig and Yorkshire", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 24(9), pp.1184~1191 (2011), 아세아·태평양축산학회
- Pengxia Niu, "Porcine insulin-like growth factor 1 (IGF1) gene polymorphisms are associated with body size variation", Genes & Genomics, 35(4), pp.523~528 (2013), 한국유전학회[DOI]