SLA Homozygous Korean Native Pigs and Their Inbreeding Status Deduced from the Microsatellite Marker Analysis
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The porcine MHC(Major Histocompatibility Complex), encoding the SLA(Swine Leukocyte Antigen) genes, is one of the most significant regions associated with immune rejection in relation to transplantation. In this study, three SLA class I(SLA-1, SLA-3, SLA-2) loci and three SLA class II(DRB1, DQB1, DQA) loci were investigated in the previously unidentified Korean native pig (KNP) population that was closely inbred in the Livestock Technology Research Station in Cheongyang, Korea. Total thirteen KNPs from four generations were genotyped for the SLA alleles and haplotypes were investigated using PCR-SSP(Sequence- Specific Primer) method. The results showed that all of these KNPs had Lr-56.30/56.30 homozygous haplotype, indicating high level of inbreeding in the SLA genes. The inbreeding status of these animals was also investigated using microsatellite(MS) markers. From the 50 MS markers investigated, 17 MS markers were fixed in all generations and the fixed alleles are increased as 26 loci for the fourth generation. Two MS markers, S0069 and SW173, were heterozygous for all the animals tested. Observed and expected heterozygosities were calculated and the average inbreeding coefficients for each generation were also calculated. In the fourth generation, the average inbreeding coefficients was 0.732 and this may increase with further inbreeding process. Analysis of the SLA haplotypes and MS alleles can give important information for breeding the pigs for xenotransplantation studies.
Abstract
The porcine MHC(Major Histocompatibility Complex), encoding the SLA(Swine Leukocyte Antigen) genes, is one of the most significant regions associated with immune rejection in relation to transplantation. In this study, three SLA class I(SLA-1, SLA-3, SLA-2) loci and three SLA class II(DRB1, DQB1, DQA) loci were investigated in the previously unidentified Korean native pig (KNP) population that was closely inbred in the Livestock Technology Research Station in Cheongyang, Korea. Total thirteen KNPs from four generations were genotyped for the SLA alleles and haplotypes were investigated using PCR-SSP(Sequence- Specific Primer) method. The results showed that all of these KNPs had Lr-56.30/56.30 homozygous haplotype, indicating high level of inbreeding in the SLA genes. The inbreeding status of these animals was also investigated using microsatellite(MS) markers. From the 50 MS markers investigated, 17 MS markers were fixed in all generations and the fixed alleles are increased as 26 loci for the fourth generation. Two MS markers, S0069 and SW173, were heterozygous for all the animals tested. Observed and expected heterozygosities were calculated and the average inbreeding coefficients for each generation were also calculated. In the fourth generation, the average inbreeding coefficients was 0.732 and this may increase with further inbreeding process. Analysis of the SLA haplotypes and MS alleles can give important information for breeding the pigs for xenotransplantation studies.
참고문헌 (19)
- Ando, A., "Rapid assignment of the swine major histocompatibility complex(SLA) class I and II genotypes in Clawn miniature swine using PCR-SSP and PCR-RFLP methods", Xenotransplantation, 12, pp.121~126 (2005)
- Han-Ok Cho, "Establishment of a Resource Population of SLA Haplotype-Defined Korean Native Pigs", 분자와 세포, 29(5), pp.493~499 (2010), 한국분자세포생물학회
- Gautschi, C., "Influence of major histocom- patibility complex on reproduction and production traits in swine", Animal Genetics, 21, pp.161~170 (1990)
- Ho, C. S., "Nomenclature for factors of the SLA system, update 2008", Tissue Antigens, 73, pp.307~315 (2009)
- Ho, C. S., "Molecular characterization of swine leukocyte antigen class I genes in outbred pig populations", Animal Genetics, 40, pp.468~478 (2009)
- Ho, C. S., "Molecular characterization of swine leukocyte antigen(SLA) class II genes in outbred pig populations", Animal Genetics, 41, pp.428~432 (2010)
- Lunney, J. K., "Immunogenetic analysis of Trichinella spiralis infections in swine", Veterinary Parasitology, 29, pp.179~193 (1988)
- Lee, J. H., "Characterization of the swine major histocompatibility complex alleles at eight loci in Westran pigs", Xenotransplantation, 12, pp.303~307 (2005)
- Mallard, B. A., "Genetic and other effects on antibody and cell mediated immune response in swine leukocyte antigen(SLA)-defined miniature pigs", Animal Genetics, 20, pp.167~178 (1989)
- Martens, G. W., "Rapid assignment of swine leukocyte antigen haplotypes in pedigreed herds using a polymerase chain reaction-based assay", Immunogenetics, 55, pp.395~401 (2003)
- Naziruddin, B., "HLA antibodies present in the sera of sensitized patients awaiting renal transplant are also reactive to swine leukocyte antigens", Transplantation, 66, pp.1074~1080 (1998)
- Remard, C., "Possible relationships between SLA and porcine reproduction", Reprodution, Nutrition, Development, 29, pp.569~576 (1989)
- Sachs, D. H., "Transplantation in miniature swine. I. Fixation of the major histocompatibility complex", Trans- plantation, 22, pp.559~567 (1976)
- Shishido, S., "Recognition of porcine major histocompatibility complex class I antigens by human CD8+ cytolytic T cell clones", Transplantation, 64, pp.340~346 (1997)
- Smith, D. M., "Nomenclature for factors of the swine leukocyte antigen class II system", Tissue Antigens, 66, pp.623~639 (2005)
- Smith, D. M., "Nomenclature for factors of the SLA class-I system", Tissue Antigens, 65, pp.136~149 (2005)
- Seo, O. K., "Assignment of the SLA alleles and reproductive potential of selective breeding Duroc pig lines", Xenotransplantation, 15, pp.390~397 (2008)
- Xu, X. C., "Allele-specific and peptide-dependent recognition of swine leukocyte antigen class I by human cytotoxic T-cell clones", Transplantation, 68, pp.473~479 (1999)
- Yamada, K., "Human antiporcine xenogeneic T cell response. Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition", Journal of Immunology, 155, pp.5249~5256 (1995)