Medicinal herb extracts ameliorate impaired growth performance and intestinal lesion of newborn piglets challenged with the virulent porcine epidemic diarrhea virus
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The objective of this study was to evaluate effects of a combined use of extracts of medicinal herbs Taraxaumi mongolicum, Viola yedoensis Makino, Rhizoma coptidis, and Radix isatidis (MYCI) on porcine epidemic diarrhea (PED). Twenty-two 3-day-old piglets received an oral challenge with 3 × 103.5 TCID50 of the virulent PED virus (PEDV) in PBS or PBS only and daily oral administration of 60 mg of the MYCI mixture suspended in milk replacer or the vehicle for 7 days in a 2 × 2 factorial arrangement of treatments. Average daily gain (ADG) increased (p < 0.05) in response to the MYCI treatment in the PEDV-challenged piglets (−18 vs. 7 g for the vehicle- vs. MYCI-administered group), but not in unchallenged animals (27 vs. 28 g). Diarrhea score and fecal PEDV shedding, however, were not influenced by the MYCI treatment. The PEDV challenge caused severe intestinal villus atrophy and crypt hyperplasia, both of which were alleviated by administration of the MYCI mixture as indicated by an increase in the villus height and a decrease in the crypt depth due to the treatment. Overall, medicinal herb extracts used in this study ameliorated impaired growth performance and intestinal lesion of newborn piglets challenged with the virulent PEDV. Therefore, our results suggest that the MYCI mixture could be used as a prophylactic or therapeutic agent against PED.
Abstract
The objective of this study was to evaluate effects of a combined use of extracts of medicinal herbs Taraxaumi mongolicum, Viola yedoensis Makino, Rhizoma coptidis, and Radix isatidis (MYCI) on porcine epidemic diarrhea (PED). Twenty-two 3-day-old piglets received an oral challenge with 3 × 103.5 TCID50 of the virulent PED virus (PEDV) in PBS or PBS only and daily oral administration of 60 mg of the MYCI mixture suspended in milk replacer or the vehicle for 7 days in a 2 × 2 factorial arrangement of treatments. Average daily gain (ADG) increased (p < 0.05) in response to the MYCI treatment in the PEDV-challenged piglets (−18 vs. 7 g for the vehicle- vs. MYCI-administered group), but not in unchallenged animals (27 vs. 28 g). Diarrhea score and fecal PEDV shedding, however, were not influenced by the MYCI treatment. The PEDV challenge caused severe intestinal villus atrophy and crypt hyperplasia, both of which were alleviated by administration of the MYCI mixture as indicated by an increase in the villus height and a decrease in the crypt depth due to the treatment. Overall, medicinal herb extracts used in this study ameliorated impaired growth performance and intestinal lesion of newborn piglets challenged with the virulent PEDV. Therefore, our results suggest that the MYCI mixture could be used as a prophylactic or therapeutic agent against PED.
참고문헌 (36)
- Murphy FA, "Veterinary Virology", pp.23~42 (1999), Academic press
- Weiss SR, "Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus", Microbiol Mol Biol Rev, 69(4), pp.635~664 (2005)
- 박봉균, "Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection", Journal of Veterinary Science, 6(4), pp.349~352 (2005), 대한수의학회
- Debouck P, "Experimental infection of pigs with a new porcine enteric coronavirus, CV 777", Am J Vet Res, 41(2), pp.219~223 (1980)
- Park SJ, "Molecular epidemiology and phylogenetic analysis of porcine epidemic diarrhea virus(PEDV)field isolates in Korea", Arch Virol, 158(7), pp.1533~1541 (2013)
- Song D, "Porcine epidemic diarrhoea virus : a comprehensive review of molecular epidemiology, diagnosis, and vaccines", Virus Genes, 44(2), pp.167~175 (2012)
- Kim O, "Application of reverse transcription polymerase chain reaction to detect porcine epidemic diarrhea virus in Vero cell culture", J Vet Diagn Invest, 11(6), pp.537~538 (1999)
- Pansaert MB, "A new coronavirus-like particle associated with diarrhea in swine", Arch Virol, 58, pp.243~247 (1978)
- Briskin DP, "Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health", Plant Physiol, 124(2), pp.507~514 (2000)
- Cowan MM, "Plant products as antimicrobial agents", Clin Microbiol Rev, 12(4), pp.564~582 (1999)
- Jassim SA, "Novel antiviral agents : a medicinal plant perspective", J Appl Microbiol, 95(3), pp.412~427 (2003)
- Wu CJ, "Inhibition of severe acute respiratory syndrome coronavirus replication by niclosamide", Antimicrob Agents Chemother, 48(7), pp.2693~2696 (2004)
- Lu HM, "Anti-inflammatory effect of Houttuynia cordata injection", J Ethnopharmacol, 104(1–2), pp.245~249 (2006)
- Kim O, "Comparison of reverse transcription polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine epidemic diarrhea virus in pigs", Can J Vet Res, 66(2), pp.112~116 (2002)
- Kim HY, "In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex", J Clin Virol, 41(2), pp.122~128 (2008)
- Han H, "Inhibitory effect of aqueous Dandelion extract on HIV-1 replication and reverse transcriptase activity", BMC Complement Altern Med, 11(112-6882), pp.11~112 (2011)
- Park KJ, "Evaluation of in vitro antiviral activity in methanol extracts against influenza virus type A from Korean medicinal plants", Phytother Res, 17, pp.1059~1063 (2003)
- Shi S, "Identification of antioxidants from Taraxacum mongolicum by high-performance liquid chromatography-diode array detection-radical-scavenging detection-electrospray ionization mass spectrometry and nuclear magnetic resonance experiments", J Chromatogr A, 1209(1–2), pp.145~152 (2008)
- Li C, "Investigating the immunomodulatory effects of Chinese herbs on mouse", J Immunol, 182, pp.43.16~ (2009)
- Xie C, "Antibacterial activity of the Chinese traditional medicine, Zi Hua Di Ding", Phytother Res, 18(6), pp.497~500 (2004)
- Chin LW, "Anti-herpes simplex virus effects of berberine from Coptidis rhizoma, a major component of a Chinese herbal medicine, Ching-Wei-San", Arch Virol, 155(12), pp.1933~1941 (2010)
- Kong WJ, "Spectrum-effect relationships between ultra performance liquid chromatography fingerprints and anti-bacterial activities of Rhizoma coptidis", Anal Chim Acta, 634(2), pp.279~285 (2009)
- Shin EK, "The anti-inflammatory effects of a methanolic extract from Radix Isatidis in murine macrophages and mice", Inflammation, 33(2), pp.110~118 (2010)
- Chen X, "Houttuynia cordata blocks HSV infection through inhibition of NF-kappaB activation", Antiviral Res, 92(2), pp.341~345 (2011)
- Zhao YL, "Effect of Radix isatidis polysaccharides on immunological function and expression of immune related cytokines in mice", Chin J Integr Med, 14(3), pp.207~211 (2008)
- Kwon CH, "Effects of dietary supplementation of lipid-encapsulated zinc oxide on colibacillosis, growth and intestinal morphology in weaned piglets challenged with enterotoxigenic Escherichia coli", Anim Sci J, 85(8), pp.805~813 (2014)
- Vlasova AN, "Distinct characteristics and complex evolution of PEDV strains, North America, May 2013-February 2014", Emerg Infect Dis, 20(10), pp.1620~1628 (2014)
- Lee JH, "Porcine epidemic diarrhea virus infection: Inhibition by polysaccharide from Ginkgo biloba exocarp and mode of its action", Virus Res, 195C, pp.148~152 (2014)
- Madson DM, "Pathogenesis of porcine epidemic diarrhea virus isolate (US", Iowa, 2013) in 3-week-old weaned pigs, pp.Vet Microbiol, 2014[DOI]
- Kim O, "Experimental infection of piglets with a korean strain of porcine epidemic diarrhoea virus", J Comp Pathol, 129(1), pp.55~60 (2003)
- Coussement W, "Pathology of experimental CV777 coronavirus enteritis in piglets. I. Histological and histochemical study", Vet Pathol, 19(1), pp.46~56 (1982)
- Naithani R, "Antiviral activity of phytochemicals : a comprehensive review", Mini Rev Med Chem, 8(11), pp.1106~1133 (2008)
- Liu J, "A new inositol triester from Taraxacum mongolicum", Nat Prod Res, 28(7), pp.420~423 (2014)
- Sun Y, "Study on antibacterial active components from Viola yedoensis", Zhongguo Zhongyao Zazhi, 36(19), pp.2666~2671 (2011)
- YE W, "screening of eleven chemical constituents from Radix isatidis for antiviral activity", Afr J Pharm and Pharmaco, 5(16), pp.1933~1936 (2011)
- Shi SY, "Studies on chemical constituents from herbs of Taraxacum mongolicum", Zhongguo Zhong Yao Za Zhi, 33(10), pp.1147~1157 (2008)