Effect of stocker management program on beef cattle skeletal muscle growth characteristics, satellite cell activity, and paracrine signaling impact on preadipocyte differentiation
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The objective of this study was to determine the effect of different stocker management programs on skeletal muscle development and growth characteristics, satellite cell (SC) activity in growing-finishing beef cattle as well as the effects of SC-conditioned media on preadipocyte gene expression and differentiation. Fall-weaned Angus steers (n = 76; 258 ± 28 kg) were randomly assigned to 1 of 4 stocker production systems: 1) grazing dormant native range (NR) supplemented with a 40% CP cottonseed meal-based supplement (1.02 kg ∙ steer–1 ∙ d–1) followed by long-season summer grazing (CON, 0.46 kg/d); 2) grazing dormant NR supplemented with a ground corn and soybean meal-based supplement fed at 1% of BW followed by short-season summer grazing (CORN, 0.61 kg/d); 3) grazing winter wheat pasture (WP) at high stocking density (3.21 steers/ha) to achieve a moderate rate of gain (LGWP, 0.83 kg/d); and 4) grazing winter WP at low stocking density (0.99 steers/ha) to achieve a high rate of gain (HGWP, 1.29 kg/d). At the end of the stocker (intermediate harvest, IH) and finishing (final harvest, FH) phases, 4 steers / treatment were harvested and longissimus muscles (LM) sampled for cryohistological immunofluorescence analysis and SC culture assays. At IH, WP steers had greater LM fiber cross-sectional area than NR steers; however, at FH, the opposite was observed (p < 0.0001). At IH, CORN steers had the lowest Myf-5+:Pax7+ SC density (p = 0.020), while LGWP steers had the most Pax7+ SC (p = 0.043). At FH, CON steers had the highest LM capillary density (p = 0.003) and their cultured SC differentiated more readily than all other treatments (p = 0.017). At FH, Pax7 mRNA was more abundant in 14 d-old SC cultures from HGWP cattle (p = 0.03). Preadipocytes exposed to culture media from proliferating SC cultures from WP cattle isolated at FH had more PPARγ (p = 0.037) and less FABP4 (p = 0.030) mRNA expression compared with NR cattle. These data suggest that different stocker management strategies can impact skeletal muscle growth, SC function, and potentially impact marbling development in growing-finishing beef cattle.
Abstract
The objective of this study was to determine the effect of different stocker management programs on skeletal muscle development and growth characteristics, satellite cell (SC) activity in growing-finishing beef cattle as well as the effects of SC-conditioned media on preadipocyte gene expression and differentiation. Fall-weaned Angus steers (n = 76; 258 ± 28 kg) were randomly assigned to 1 of 4 stocker production systems: 1) grazing dormant native range (NR) supplemented with a 40% CP cottonseed meal-based supplement (1.02 kg ∙ steer–1 ∙ d–1) followed by long-season summer grazing (CON, 0.46 kg/d); 2) grazing dormant NR supplemented with a ground corn and soybean meal-based supplement fed at 1% of BW followed by short-season summer grazing (CORN, 0.61 kg/d); 3) grazing winter wheat pasture (WP) at high stocking density (3.21 steers/ha) to achieve a moderate rate of gain (LGWP, 0.83 kg/d); and 4) grazing winter WP at low stocking density (0.99 steers/ha) to achieve a high rate of gain (HGWP, 1.29 kg/d). At the end of the stocker (intermediate harvest, IH) and finishing (final harvest, FH) phases, 4 steers / treatment were harvested and longissimus muscles (LM) sampled for cryohistological immunofluorescence analysis and SC culture assays. At IH, WP steers had greater LM fiber cross-sectional area than NR steers; however, at FH, the opposite was observed (p < 0.0001). At IH, CORN steers had the lowest Myf-5+:Pax7+ SC density (p = 0.020), while LGWP steers had the most Pax7+ SC (p = 0.043). At FH, CON steers had the highest LM capillary density (p = 0.003) and their cultured SC differentiated more readily than all other treatments (p = 0.017). At FH, Pax7 mRNA was more abundant in 14 d-old SC cultures from HGWP cattle (p = 0.03). Preadipocytes exposed to culture media from proliferating SC cultures from WP cattle isolated at FH had more PPARγ (p = 0.037) and less FABP4 (p = 0.030) mRNA expression compared with NR cattle. These data suggest that different stocker management strategies can impact skeletal muscle growth, SC function, and potentially impact marbling development in growing-finishing beef cattle.
참고문헌 (37)
- "Optimum forage use in beef cattle growing and finishing diets" (1991)[URL]
- Loken BA, "Growing rate of gain on subsequent feedlot performance, meat, and carcass quality of beef steers", J Anim Sci, 87, pp.3791~3797 (2009)
- Hersom MJ, "Effect of live weight gain of steers during winter grazing: I. feedlot performance, carcass characteristics, and body composition of beef steers", J Anim Sci, 82, pp.262~272 (2004)
- Anderson P, "Non-genetic factors that affect quality grade of fed cattle", Proceedings Beef Improvement Federation 39th Annual Research Symposium and Annual Meeting (2007), Fort Collins, CO
- Seideman SC, "The effects of sex condition, genotype and diet on bovine muscle fiber characteristics", Meat Sci, 17, pp.55~72 (1986)
- Vestergaard M, "Influence of feeding intensity, grazing and finishing feeding on meat and eating quality of young bulls and the relationship between muscle fibre characteristics, fibre fragmentation and meat tenderness", Meat Sci, 54, pp.187~195 (2000)
- Lehnert SA, "Gene expression profiling of bovine skeletal muscle in response to and during recovery from chronic and severe undernutrition", J Anim Sci, 84, pp.3239~3250 (2006)
- Greenwood PL, "Bovine myofiber characteristics are influenced by postweaning nutrition", J Anim Sci, 87, pp.3114~3123 (2009)
- Shibata M, "Differential expression of the skeletal muscle proteome in grazed cattle", J Anim Sci, 87, pp.2700~2708 (2009)
- Blumer TN, "Nature and variability of marbling deposits in longissimus dorsi muscle of beef carcasses", J Anim Sci, 21, pp.935~942 (1962)
- Moody WG, "A quantitative and morphological study of bovine longissimus fat cells", J Food Sci, 33, pp.47~52 (1968)
- Melton C, "Histological relationships of muscle biopsies to bovine meat quality and carcass composition", J Anim Sci, 38, pp.24~31 (1974)
- Melton CC, "Histochemical relationships of muscle biopsies with bovine muscle quality and composition", J Anim Sci, 40, pp.451~456 (1975)
- Moss FP, "Satellite cells as the source of nuclei in muscles of growing rats", Anat Rec, 170, pp.421~435 (1971)
- Harper GS, "How might marbling begin?", Austr J Exp Ag, 44, pp.653~662 (2004)
- Sharman ED, "Effect of rate of body weight gain in steers during the stocker phase. I. growth, partitioning of fat among depots, and carcass characteristics of growing-finishing beef cattle", J Anim Sci, 91, pp.4322~4335 (2013)
- Day K, "A distinct profile of myogenic regulatory factor detection within Pax7+ cells at S phase supports a unique role of Myf5 during posthatch chicken myogenesis", Dev Dyn, 238, pp.1001~1009 (2009)
- Day K, "Nestin-GFP reporter expression defines the quiescent state of skeletal muscle satellite cells", Dev Biol, 304, pp.246~259 (2007)
- Hathaway MR, "Effect of transforming growth factor beta-1 on ovine satellite cell proliferation and fusion", J Cell Physiol, 146, pp.435~441 (1991)
- Frey RS, "Growth factor responsiveness of primary satellite cell cultures from steers implanted with trembolone acetate and estradiol-17beta", Basic Appl Myol, 5, pp.71~79 (1995)
- Johnson BJ, "Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant", J Anim Sci, 76, pp.2779~2786 (1998)
- Rozen S, "Primer3 on the WWW for general users and for biologist programmers", Methods Mol Biol, 132, pp.365~386 (2000)
- Granfar RM, "Optimised real-time quantitative PCR assays for RANKL regulated genes", Mol Cell Probes, 19, pp.119~126 (2005)
- Taylor-Jones JM, "Activation of an adipogenic program in adult myoblasts with age", Mech Ageing Dev, 123, pp.649~661 (2002)
- Guan Y, "p130", p107 expression distinguishes adipogenic potential in primary myoblasts based on age, 2002, pp.296, 1340~1345
- Vandesompele J, "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes", Genome Biol, 3, pp.research00~ (2002)
- Koopman R, "Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids", Histochem Cell Biol, 116, pp.63~68 (2001)
- Starkey JD, "Skeletal muscle satellite cells are committed to myogenesis and do not spontaneously adopt nonmyogenic fates", J Histochem Cytochem, 59, pp.33~46 (2011)
- Sharman ED, "Effect of rate of body weight gain of steers during the stocker phase. II. visceral organ mass and body composition of growing-finishing beef cattle", J Anim Sci, 91, pp.2355~2366 (2013)
- Lancaster PA, "Effect of rate of weight gain of steers during the stocker phase. III. gene expression of adipose tissues and skeletal muscle in growing-finishing beef cattle", J Anim Sci, 92, pp.1462~1472 (2014)
- Lancaster PA, "Effect of rate of weight gain of steers during the stocker phase. IV. rumen fermentation characteristics and expression of genes involved in substrate utilization for fatty acid synthesis in adipose tissues of growing-finishing beef cattle", J Anim Sci, 93, pp.3055~3065 (2015)
- Ridenour KW, "Feedlot performance and carcass characteristics of beef steers grown and finished under different nutrition and management programs", J Anim Sci, 54, pp.1115~1119 (1982)
- Choat WT, "Influence of grazing dormant native range or winter wheat pasture on subsequent finishing cattle performance, carcass characteristics, and ruminal metabolism", J Anim Sci, 81, pp.3191~3201 (2003)
- Rosen ED, "Transcriptional regulation of adipogenesis", Genes Dev, 14, pp.1293~1307 (2000)
- Smith SB, "Relative contributions of acetate, lactate and glucose to lipogenesis in bovine intramuscular and subcutaneous adipose tissue", J Nutr, 114, pp.792~800 (1984)
- Yates DT, "Developmental programming in response to intrauterine growth restriction impairs myoblast function and skeletal muscle metabolism", J Pregnancy, 2012, pp.631038~ (2012)
- Yan X, "Developmental programming of fetal skeletal muscle and adipose tissue development", J Genomics, 1, pp.29~38 (2013)