mRNA expression of myogenicadipogenic makers and adipocyte in skeletal muscle of Hanwoo calves at newborn and 6 months of age
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This study was conducted to compare the mRNA expression levels of myogenic-adipogenic makers in the skeletal muscle and adipocytes formation, body weight, rumen weight, and papilla length on Hanwoo calves at newborn and 6 months of age. Animals used three newborn Hanwoo calves (NC) and three Hanwoo calves 6 months of age (SC). Body weight and rumen weight were significantly increased in SC compared to NC (p < 0.01), and papilla length was longer about 10-fold in SC than NC. Adipocytes was possible to visually identify more adipocytes in SC compared to NC, and were mainly formed around the blood vessels. mRNA expression of myogenin, myosin heavy chain 1 and myosin heavy chain 2A in both longissimus dorsi (LD) and semimembranosus (SM) was found to increase with calves growth (p < 0.01), and it was confirmed that have higher levels of mRNA expression in SM than LD. In LD tissues, the mRNA expression of stearoyl-CoA desaturase (SCD, p < 0.03) and peroxisome proliferator activated receptor γ (PPARγ, p < 0.04) was significantly higher in SC than NC. In SM tissues, mRNA expression levels of SCD (p < 0.02) and CCAAT/enhancer binding protein β (C/EBPβ, p < 0.01) were higher in SC than NC, and also mRNA expression levels of PPARγ increased, but there was no significant difference. Thus, the calves period suggests that it is an important step in the development of the rumen and the myogenesis and adipogenesis.
Abstract
This study was conducted to compare the mRNA expression levels of myogenic-adipogenic makers in the skeletal muscle and adipocytes formation, body weight, rumen weight, and papilla length on Hanwoo calves at newborn and 6 months of age. Animals used three newborn Hanwoo calves (NC) and three Hanwoo calves 6 months of age (SC). Body weight and rumen weight were significantly increased in SC compared to NC (p < 0.01), and papilla length was longer about 10-fold in SC than NC. Adipocytes was possible to visually identify more adipocytes in SC compared to NC, and were mainly formed around the blood vessels. mRNA expression of myogenin, myosin heavy chain 1 and myosin heavy chain 2A in both longissimus dorsi (LD) and semimembranosus (SM) was found to increase with calves growth (p < 0.01), and it was confirmed that have higher levels of mRNA expression in SM than LD. In LD tissues, the mRNA expression of stearoyl-CoA desaturase (SCD, p < 0.03) and peroxisome proliferator activated receptor γ (PPARγ, p < 0.04) was significantly higher in SC than NC. In SM tissues, mRNA expression levels of SCD (p < 0.02) and CCAAT/enhancer binding protein β (C/EBPβ, p < 0.01) were higher in SC than NC, and also mRNA expression levels of PPARγ increased, but there was no significant difference. Thus, the calves period suggests that it is an important step in the development of the rumen and the myogenesis and adipogenesis.
참고문헌 (36)
- M. Du, "Fetal programming of skeletal muscle development in ruminant animals1", Journal of Animal Science, 88(suppl_13), pp.E51~E60 (2010), Oxford University Press (OUP)[DOI]
- R.L. Baldwin, "Rumen Development, Intestinal Growth and Hepatic Metabolism In The Pre- and Postweaning Ruminant", Journal of Dairy Science, 87, pp.E55~E65 (2004), American Dairy Science Association[DOI]
- 이승환, "유전체정보활용 한우개량효율 증진", Korean Journal of Agricultural Science, 42(4), pp.397~406 (2015), 농업과학연구소[DOI]
- 이승환, "한우 비육 전·후기의 등심조직에 있어서 지방합성 유전자 발현", 한국축산학회지, 48(3), pp.345~352 (2006), 한국축산학회
- Stevens CE, "Physiology of digestion and metabolism in the ruminant", pp.101~112 (1970), Oriel Press
- Gálfi P, "Physiological aspects of digestion and metabolism in ruminants: proceedings of the Seventh International Symposium on Ruminant Physiology", Physiological aspects of digestion and metabolism in ruminants: proceedings of the Seventh International Symposium on Ruminant Physiology (1991), Academic Press
- Tomohiro SATO, "Effect of Sugar Supplementation on Rumen Protozoa Profile and Papillae Development in Retarded Growth Calves", Journal of Veterinary Medical Science, 72(11), pp.1471~1474 (2010), Japanese Society of Veterinary Science[DOI]
- Lyford SJ, "The ruminant animal", pp.44~63 (1988), Prentice Hall
- Elie Jami, "Exploring the bovine rumen bacterial community from birth to adulthood", The ISME Journal, 7(6), pp.1069~1079 (2013), Springer Science and Business Media LLC[DOI]
- Brieuc Morvan, "Establishment of hydrogen-utilizing bacteria in the rumen of the newborn lamb", FEMS Microbiology Letters, 117(3), pp.249~256 (1994), Oxford University Press (OUP)[DOI]
- L. Abecia, "Feeding management in early life influences microbial colonisation and fermentation in the rumen of newborn goat kids", Animal Production Science, 54(9), pp.1449~ (2014), CSIRO Publishing[DOI]
- Mokshata Gupta, "Nutritional drivers of rumen development: A review", Agricultural Reviews, 37(2), pp.148~153 (2016), Agricultural Research Communication Center[DOI]
- R.L. Baldwin, "Rumen Development, Intestinal Growth and Hepatic Metabolism In The Pre- and Postweaning Ruminant", Journal of Dairy Science, 87, pp.E55~E65 (2004), American Dairy Science Association[DOI]
- T. Hamada, "Factors Influencing Growth of Rumen, Liver, and Other Organs in Kids Weaned from Milk Replacers to Solid Foods", Journal of Dairy Science, 59(6), pp.1110~1118 (1976), American Dairy Science Association[DOI]
- W.P. Flatt, "Influence of Purified Materials on the Development of the Ruminant Stomach", Journal of Dairy Science, 41(11), pp.1593~1600 (1958), American Dairy Science Association[DOI]
- Jamila Soomro, "Synchronous and Time-Dependent Expression of Cyclins, Cyclin-Dependant Kinases, and Apoptotic Genes in the Rumen Epithelia of Butyrate-Infused Goats", Frontiers in Physiology, 9, pp.496~ (2018), Frontiers Media SA[DOI]
- X.Z. Zhang, "Calcium propionate supplementation improves development of rumen epithelium in calves via stimulating G protein-coupled receptors", Animal, 12(11), pp.2284~2291 (2018), Elsevier BV[DOI]
- R. Žitnan, "Influence of dietary concentrate to forage ratio on the development of rumen mucosa in calves", Archiv für Tierernaehrung, 51(4), pp.279~291 (1998), Informa UK Limited[DOI]
- M. S. Duarte, "Maternal overnutrition enhances mRNA expression of adipogenic markers and collagen deposition in skeletal muscle of beef cattle fetuses1", Journal of Animal Science, 92(9), pp.3846~3854 (2014), Oxford University Press (OUP)[DOI]
- Gotoh T, "Metabolic imprinting effect in beef production: influence of nutrition manipulation during an early growth stage on carcass characteristics and intramuscular fat content of longissimus muscle in Wagyu (Japanese Black)", Proceeding of the 3rd EAAP International Symposium on Energy and Protein Metabolism and Nutrition (2010)
- A. E. Wertz, "Early-weaning and postweaning nutritional management affect feedlot performance, carcass merit, and the relationship of 12th-rib fat, marbling score, and feed efficiency among Angus and Wagyu heifers", Journal of Animal Science, 80(1), pp.28~37 (2002), Oxford University Press (OUP)[DOI]
- N. A. Pyatt, "Factors affecting carcass value and profitability in early-weaned Simmental steers: I. Five-year average pricing", Journal of Animal Science, 83(12), pp.2918~2925 (2005), Oxford University Press (OUP)[DOI]
- N. A. Pyatt, "Factors affecting carcass value and profitability in early-weaned Simmental steers: II. Days on feed endpoints and sorting strategies", Journal of Animal Science, 83(12), pp.2926~2937 (2005), Oxford University Press (OUP)[DOI]
- Arnold HH, "Genetics of muscle determination and development", Curr Top Dev Biol, 48, pp.129~164 (1999)[DOI]
- C. M. Dwyer, "Muscle cellularity and postnatal growth in the pig1", Journal of Animal Science, 71(12), pp.3339~3343 (1993), Oxford University Press (OUP)[DOI]
- Gary E Lyons, "In Situ Analysis of Muscle Gene Expression in Mouse Embryos", Journal of Animal Science, 74(suppl_2), pp.1~ (1996), Oxford University Press (OUP)[DOI]
- G. Goldspink, "Gene expression in skeletal muscle in response to stretch and force generation", American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 262(3), pp.R356~R363 (1992), American Physiological Society[DOI]
- 백수정, "근육의 가소성에 대한 고찰", 대한물리치료학회지, 15(2), pp.100~110 (2003), 대한물리치료학회
- Kenneth M. Baldwin, "Skeletal muscle plasticity: cellular and molecular responses to altered physical activity paradigms", American Journal of Physical Medicine & Rehabilitation, 81(Supplement), pp.S40~S51 (2002), Ovid Technologies (Wolters Kluwer Health)[DOI]
- L. Lefaucheur, "Muscle fiber plasticity in farm mammals", Journal of Animal Science, 77(E-Suppl), pp.1~ (2000), Oxford University Press (OUP)[DOI]
- Regina P Brun, "Adipocyte differentiation: a transcriptional regulatory cascade", Current Opinion in Cell Biology, 8(6), pp.826~832 (1996), Elsevier BV[DOI]
- Jonathan K. Hamm, "A Role for C", EBPβ in Regulating Peroxisome Proliferator-activated Receptor γ Activity during Adipogenesis in 3T3-L1 Preadipocytes, 2001, pp.276(21), 18464~18471[DOI]
- Danilo S. Cianzio, "Adipose Tissue Growth and Cellularity: Changes in Bovine Adipocyte Size and Number", Journal of Animal Science, 60(4), pp.970~976 (1985), Oxford University Press (OUP)[DOI]
- G. S. Harper, "How might marbling begin?", Australian Journal of Experimental Agriculture, 44(7), pp.653~ (2004), CSIRO Publishing[DOI]
- Hei Sook Sul, "NUTRITIONAL AND HORMONAL REGULATION OF ENZYMES IN FAT SYNTHESIS: Studies of Fatty Acid Synthase and Mitochondrial Glycerol-3-Phosphate Acyltransferase Gene Transcription", Annual Review of Nutrition, 18(1), pp.331~351 (1998), Annual Reviews[DOI]
- Fumio Ebara, "Intensive Nursing and Feeding During the Early Growth Period Altered Intramuscular Adipogenesis in Crossbred Steers (Japanese Black Male x Holstein Female)", Journal of Animal and Veterinary Advances, 9(6), pp.982~989 (2010), Medwell Publications[DOI]