The difference of castration timing of Korean Hanwoo bulls does not significantly affect the carcass characteristics
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It is already well known that castration improves marbling quality but exact timing of castration is still highly debated in beef cattle production industry. After castration, blood hormonal changes occur in steer and objective of this study was to investigate the effects of growth hormone (GH) levels on adipocyte differentiation in stromal vascular cells (SVCs) and transdifferentiation into adipocytes in C2C12 myoblasts. Total GH concentrations were measured via enzyme-linked immunosorbent assay (ELISA) in 24 male calves and 4 female calves. Cell proliferation, cellular triglyceride (TG) accumulation, and the cell’s lipolytic capability were measured in C2C12 myoblasts and SVCs. Myogenic, adipogenic, and brown adipocyte-specific gene expression was measured via real-time polymerase chain reaction (PCR) using SYBR green. Serum GH levels were the highest in late-castrated calves. Treatment with 5 ng/mL GH resulted in greater TG accumulation as well as increased CCAAT-enhancer-binding protein (C/EBP)α and peroxisome proliferator-activated receptor (PPAR)γ expression compared to that after treatment with 15 ng/mL GH. Treatment with 5 ng/mL GH also resulted in lower myogenin (myo)G and myoD expression compared to that after treatment with 15 ng/mL GH. The expression of bone morphogenetic protein (BMP) 7 after treatment with 5 ng/ mL GH was higher than that after treatment with 15 ng/mL GH. But carcass characteristics data showed no significant difference between early and late castrated steers. Therefore, our results indicate that castration timing does not seem to be inevitable determinate of carcass qualities, particularly carcass weight and marbling score in Hanwoo beef cattle.
Abstract
It is already well known that castration improves marbling quality but exact timing of castration is still highly debated in beef cattle production industry. After castration, blood hormonal changes occur in steer and objective of this study was to investigate the effects of growth hormone (GH) levels on adipocyte differentiation in stromal vascular cells (SVCs) and transdifferentiation into adipocytes in C2C12 myoblasts. Total GH concentrations were measured via enzyme-linked immunosorbent assay (ELISA) in 24 male calves and 4 female calves. Cell proliferation, cellular triglyceride (TG) accumulation, and the cell’s lipolytic capability were measured in C2C12 myoblasts and SVCs. Myogenic, adipogenic, and brown adipocyte-specific gene expression was measured via real-time polymerase chain reaction (PCR) using SYBR green. Serum GH levels were the highest in late-castrated calves. Treatment with 5 ng/mL GH resulted in greater TG accumulation as well as increased CCAAT-enhancer-binding protein (C/EBP)α and peroxisome proliferator-activated receptor (PPAR)γ expression compared to that after treatment with 15 ng/mL GH. Treatment with 5 ng/mL GH also resulted in lower myogenin (myo)G and myoD expression compared to that after treatment with 15 ng/mL GH. The expression of bone morphogenetic protein (BMP) 7 after treatment with 5 ng/ mL GH was higher than that after treatment with 15 ng/mL GH. But carcass characteristics data showed no significant difference between early and late castrated steers. Therefore, our results indicate that castration timing does not seem to be inevitable determinate of carcass qualities, particularly carcass weight and marbling score in Hanwoo beef cattle.
참고문헌 (33)
- Felista W. Mwangi, "Diet and Genetics Influence Beef Cattle Performance and Meat Quality Characteristics", Foods, 8(12), pp.648~ (2019), MDPI AG[DOI]
- 이보인, "Correlation of Marbling Characteristics with Meat Quality and Histochemical Characteristics in Longissimus Thoracis Muscle from Hanwoo Steers", 한국축산식품학회지, 39(1), pp.151~161 (2019), 한국축산식품학회[DOI]
- Roberto Vettor, "The origin of intermuscular adipose tissue and its pathophysiological implications", American Journal of Physiology-Endocrinology and Metabolism, 297(5), pp.E987~E998 (2009), American Physiological Society[DOI]
- Kevin J P Ryan, "Dose-dependent effects of vitamin D on transdifferentiation of skeletal muscle cells to adipose cells", Journal of Endocrinology, 217(1), pp.45~58 (2013), Bioscientifica[DOI]
- G. Bretschneider, "Effects of age and method of castration on performance and stress response of beef male cattle: A review", Livestock Production Science, 97(2-3), pp.89~100 (2005), Elsevier BV[DOI]
- Jing Wang, "Castration-induced changes in the expression profiles and promoter methylation of the GHR gene in Huainan male pigs", Animal Science Journal, 88(8), pp.1113~1119 (2017), Wiley[DOI]
- Bolamperti S, "The Role of Growth Hormone in Mesenchymal Stem Cell Commitment", International Journal of Molecular Sciences, 20(21), pp.5264~ (2019), MDPI AG[DOI]
- John J. Kopchick, "The effects of growth hormone on adipose tissue: old observations, new mechanisms", Nature Reviews Endocrinology, 16(3), pp.135~146 (2020), Springer Science and Business Media LLC[DOI]
- Jeanne L. Burton, "A review of bovine growth hormone", Canadian Journal of Animal Science, 74(2), pp.167~201 (1994), Canadian Science Publishing[DOI]
- J. Škarda, "Effect of bovine growth hormone on growth, organ weights, tissue composition and adipose tissue metabolism in young castrated male goats", Livestock Production Science, 55(3), pp.215~225 (1998), Elsevier BV[DOI]
- Seongho Han, "Adipose-Derived Stromal Vascular Fraction Cells: Update on Clinical Utility and Efficacy", Critical Reviews in Eukaryotic Gene Expression, 25(2), pp.145~152 (2015), Begell House[DOI]
- Delgerzul Baatar, "Effect of testosterone on the differentiation control of stromal vascular cells isolated from longissimus muscle of Hanwoo beef cattle", Meat Science, 159, pp.107916~ (2020), Elsevier BV[DOI]
- X. Ge, "Growth hormone stimulates protein synthesis in bovine skeletal muscle cells without altering insulin-like growth factor-I mRNA expression1", Journal of Animal Science, 90(4), pp.1126~1133 (2012), Oxford University Press (OUP)[DOI]
- K. Sarjeant, "Adipogenesis", Cold Spring Harbor Perspectives in Biology, 4(9), pp.a008417~a008417 (2012), Cold Spring Harbor Laboratory[DOI]
- Wen Luo, "Myomaker, Regulated by MYOD, MYOG and miR-140-3p, Promotes Chicken Myoblast Fusion", International Journal of Molecular Sciences, 16(11), pp.26186~26201 (2015), MDPI AG[DOI]
- Eric Meadows, "Myogenin regulates a distinct genetic program in adult muscle stem cells", Developmental Biology, 322(2), pp.406~414 (2008), Elsevier BV[DOI]
- Stephen J. Tapscott, "The circuitry of a master switch: Myod and the regulation of skeletal muscle gene transcription", Development, 132(12), pp.2685~2695 (2005), The Company of Biologists[DOI]
- Z.G. Miao, "Invited review: mesenchymal progenitor cells in intramuscular connective tissue development", Animal, 10(1), pp.75~81 (2016), Elsevier BV[DOI]
- Sarah M. Choi, "Insulin Regulates Adipocyte Lipolysis via an Akt-Independent Signaling Pathway", Molecular and Cellular Biology, 30(21), pp.5009~5020 (2010), American Society for Microbiology[DOI]
- Thomas Svava Nielsen, "Dissecting adipose tissue lipolysis: molecular regulation and implications for metabolic disease", Journal of Molecular Endocrinology, 52(3), pp.R199~R222 (2014), Bioscientifica[DOI]
- Joel A. Aronowitz, "Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue", SpringerPlus, 4(1), pp.713~ (2015), Springer Science and Business Media LLC[DOI]
- 이은주, "Gene expression profiles during differentiation and transdifferentiation of bovine myogenic satellite cells", Genes & Genomics, 34(2), pp.133~148 (2012), 한국유전학회[DOI]
- Tizhong Shan, "Distinct populations of adipogenic and myogenic Myf5-lineage progenitors in white adipose tissues", Journal of Lipid Research, 54(8), pp.2214~2224 (2013), Elsevier BV[DOI]
- N.K. Singh, "Conversion of C2C12 Myoblast into Adipoblast with Thiazolidinediones - A Possible Basis for Intramuscular Fat Generation in Meat Animals", Animal Bioscience, 20(3), pp.432~439 (2007), 아세아·태평양축산학회
- Yanbei Zhu, "Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ", Obesity, 23(5), pp.1014~1021 (2015), Wiley[DOI]
- Chao Wang, "Loss of MyoD Promotes Fate Transdifferentiation of Myoblasts Into Brown Adipocytes", EBioMedicine, 16, pp.212~223 (2017), Elsevier BV[DOI]
- Mariëtte R. Boon, "BMP7 Activates Brown Adipose Tissue and Reduces Diet-Induced Obesity Only at Subthermoneutrality", PLoS ONE, 8(9), pp.e74083~ (2013), Public Library of Science (PLoS)[DOI]
- Anastasia V. Kalinovich, "UCP1 in adipose tissues: two steps to full browning", Biochimie, 134, pp.127~137 (2017), Elsevier BV[DOI]
- Craig Porter, "Quantification of UCP1 function in human brown adipose tissue", Adipocyte, 6(2), pp.167~174 (2017), Informa UK Limited[DOI]
- Meshail Okla, "BMP7 Drives Human Adipogenic Stem Cells into Metabolically Active Beige Adipocytes", Lipids, 50(2), pp.111~120 (2015), Wiley[DOI]
- Supriya Saini, "Role of BMP7 in appetite regulation, adipogenesis, and energy expenditure", Endocrine, 48(2), pp.405~409 (2015), Springer Science and Business Media LLC[DOI]
- T. W. Knight, "Effect of interval from castration of bulls to slaughter on carcass characteristics and meat quality", New Zealand Journal of Agricultural Research, 42(3), pp.269~277 (1999), Informa UK Limited[DOI]
- S. Marti, "Effect of castration and slaughter age on performance, carcass, and meat quality traits of Holstein calves fed a high-concentrate diet1", Journal of Animal Science, 91(3), pp.1129~1140 (2013), Oxford University Press (OUP)[DOI]