Megalocytivirus 감염 해산 어류에서 나타나는 임상증상의 정량적 변화 분석
Quantitative analysis of the clinical signs in marine fish induced by Megalocytivirus infection
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In quantitative studies of clinical signs, rock bream of adults and juveniles infected with Megalocytivirus IVS-1 isolated from rock bream (Oplegnathus fasciatus) in Korea showed average 4.49±1.13 and 4.85±1.06 of spleen index respectively. In challenge experiments, Megalocytivirus IVS-1 induced 100% cumulative mortality in both adult and juvenile rock bream. However we found 60% cumulative mortality in juvenile red sea bream (Pagrus major) even after 30 days of injection, which contradicted with the results of other ratories. Interestingly, IVS-1 infected red sea bream of the same juvenile size with rock bream showed lower spleen index compared to that of rock bream. In real-time PCR, there was continuous increasing of the numbers of viral copies (2.03×10^7 copies/mg) in the spleen of juvenile rock bream infected, which were different from those in adult rock bream showing plateau level after reaching to the peak level. Moreover, enlarged cell numbers in the infected spleen were also increased continuously in the juvenile but not in adult of rock bream, even decreased after reaching to peak level. Consequently, significant differences in clinical signs : cumulative mortality. spleen index and viral copy number were found between rock bream and red sea bream, but not between adult and juvenile rock bream. Certainly quantitative pression of clinical sign as in this study may be a way to compare the progression of megalocitiviral disease more accurately in different species or physiological conditions.
Abstract
In quantitative studies of clinical signs, rock bream of adults and juveniles infected with Megalocytivirus IVS-1 isolated from rock bream (Oplegnathus fasciatus) in Korea showed average 4.49±1.13 and 4.85±1.06 of spleen index respectively. In challenge experiments, Megalocytivirus IVS-1 induced 100% cumulative mortality in both adult and juvenile rock bream. However we found 60% cumulative mortality in juvenile red sea bream (Pagrus major) even after 30 days of injection, which contradicted with the results of other ratories. Interestingly, IVS-1 infected red sea bream of the same juvenile size with rock bream showed lower spleen index compared to that of rock bream. In real-time PCR, there was continuous increasing of the numbers of viral copies (2.03×10^7 copies/mg) in the spleen of juvenile rock bream infected, which were different from those in adult rock bream showing plateau level after reaching to the peak level. Moreover, enlarged cell numbers in the infected spleen were also increased continuously in the juvenile but not in adult of rock bream, even decreased after reaching to peak level. Consequently, significant differences in clinical signs : cumulative mortality. spleen index and viral copy number were found between rock bream and red sea bream, but not between adult and juvenile rock bream. Certainly quantitative pression of clinical sign as in this study may be a way to compare the progression of megalocitiviral disease more accurately in different species or physiological conditions.
참고문헌 (17)
- Chao, C.B, "A nested PCR for the detection of grouper iridovirus in Taiwan (TGIV) in cultured hybrid grouper, giant seaperch, and largemouth bass", J. Aquatic animal health, 14, pp.104~113 (2002)
- Chao, C.B, "Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV)", Dis. Aqua. Org, 58, pp.127~142 (2004)
- Do, J.W, "Complete genomic DNA sequence of rock bream iridovirus", VIROLOGY, 325(2), pp.351~363 (2004), ACADEMIC PRESS INC ELSEVIER SCIENCE
- Hanson, L.A, "Persistence of largemouth bass virus infection in a northern Mississippi reservoir after a die-off", J. Aqua. Anim. Health, 13, pp.27~34 (2001)
- Goldberg, T.L, "Strain variation in an emerging iridovirus of warm-water fishes", J. Virol, 77, pp.8812~8818 (2003)
- He, J.G, "Complete genome analysis of the mandarinfish infectious spleen and kidney necrosis iridovirus", Virol, 291, pp.126~139 (2001)
- He, J.G, "Systemic disease caused by an iridovirus-like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China", J. Fish Dis, 23, pp.219~222 (2000)
- Inouye, K, "Iridovirus infection of cultured red sea bream, Pagrus major", Fish Pathol, 27, pp.19~27 (1992)
- Jeong, J.B, "Molecular comparison of iridoviruses isolated from marine fish cultured in Korea and imported from China", Aquaculture, 255, pp.105~116 (2006)
- Kurita, J, "Polymerase chain reation (PCR) amplification of DNA of red sea bream iridovirus (RSIV)", Fish Pathol, 33, pp.17~23 (1998)
- Mahardika, K, "Susceptibility of juvenile humpback grouper Cromileptes altivelis to grouper sleepy disease iridovirus (GSDIV)", Dis. Aqua. Org, 59, pp.1~9 (2004)
- Nakajima, K, "Biological and physico-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major", Fish Pathol, 29, pp.29~33 (1994)
- Nerland, A.H, "Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae", Dis. Aqua. Org, 73, pp.201~205 (2007)
- Oshima, S, "Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction", Dis. Aquat. Org, 32, pp.87~90 (1998)
- Pallister, J, "Development of real-time PCR assays for the detection and differentiation of Australian and European ranaviruses", J. Fish Dis, 30, pp.427~438 (2007)
- Sudthongkong, C, "Iridovirus disease in two ornamental tropical freshwater fishes: African lampeye and dwarf gourami", Dis. Aquat. Org, 48, pp.163~173 (2002)
- Wang, X.W, "Quantitative detection of a marine fish iridovirus isolated from large yellow croaker, Pseudosciaena crocea, using a molecular beacon", J. Virol. Methods, 133, pp.76~81 (2006)