4배체 참굴(Crassostrea gigas)의 정자 동결보존
Sperm Cryopreservation of Tetraploid Pacific Oysters Crassostrea gigas
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초록
The goal of this study was to evaluate the effects of cryoprotectants [dimethyl sulfoxide (DMSO), methanol, polyethylene glycol, and propylene glycol], cryoprotectant concentrations (10% and 20%), equilibration time (3, 10, and 30 min), cooling rate (3°, 5°, 7°, and 10°/min), and straw size (0.25 and 0.5 mL) for sperm cryopreservation of tetraploid Pacific oysters. There was a significant difference among the four cryoprotectants, with 10% DMSO yielding the highest post-thaw survival and activity index of sperm. A significant relationship was observed between the cryoprotectant and its concentration. The sperm with equilibration times of 30 min yielded higher post-thaw survival and activity indices than those with 3 and 10 min equilibration times. The sperm cooled at a rate of 5°/min yielded the highest post-thaw survival and activity index, and the results were significantly different from those observed for cooling at 7° and 10°/min. Post-thaw survival and activity indices of sperm using a 0.25-mL straw were significantly higher than those using a 0.5-mL straw.
Abstract
The goal of this study was to evaluate the effects of cryoprotectants [dimethyl sulfoxide (DMSO), methanol, polyethylene glycol, and propylene glycol], cryoprotectant concentrations (10% and 20%), equilibration time (3, 10, and 30 min), cooling rate (3°, 5°, 7°, and 10°/min), and straw size (0.25 and 0.5 mL) for sperm cryopreservation of tetraploid Pacific oysters. There was a significant difference among the four cryoprotectants, with 10% DMSO yielding the highest post-thaw survival and activity index of sperm. A significant relationship was observed between the cryoprotectant and its concentration. The sperm with equilibration times of 30 min yielded higher post-thaw survival and activity indices than those with 3 and 10 min equilibration times. The sperm cooled at a rate of 5°/min yielded the highest post-thaw survival and activity index, and the results were significantly different from those observed for cooling at 7° and 10°/min. Post-thaw survival and activity indices of sperm using a 0.25-mL straw were significantly higher than those using a 0.5-mL straw.
참고문헌 (33)
- Adams SL, "Cryopreser- vation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat pro- duction", Aquaculture, 242, pp.271~282 (2004)
- Babiak I, "Computercontrolled freezing of rainbow trout Oncorhnchus mykiss (Walbaum) spermatozoa for rou- tine programmes", Aquacult Res, 30, pp.707~710 (1999)
- Billard R, "Cryobanking the Genetic Resource: Wildlife Conservation for the Future", pp.143~170 (2001), Taylor & Francis
- Cabrita E, "Cryo- preservation of rainbow trout sperm in large volume straws: application to large scale fertilization", Aquaculture, 201, pp.301~314 (2001)
- Christensen JM, "Refrigerated storage of channel catfish sperm", J World Aquacult Soc, 3, pp.340~346 (1996)
- Christensen JM, "Cryopreservation of channel catfish spermatozoa: effect of cryopreservation, straw size, and formulation of extender", Theriogenology, 47, pp.639~645 (1997)
- Dong Q, "Commercial-scale sperm cryopreservation of diploid and tetraploid Pacific oyster, Crassostrea gigas", Cryobiology, 50, pp.1~16 (2005)
- Dong Q, "Systematic factor optimization for sperm cryopreser- vation of tetraploid Pacific oyster, Crassostrea gigas", The- riogenology, 66, pp.387~403 (2006)
- Dong Q, "Sys- tematic factor optimization for cryopreservation of shipped sperm samples of diploid Pacific oyster, Crassostrea gigas (Thunberg)", Cryobiology, 51, pp.176~197 (2005)
- Dong Q, "Spermatozoal ultra- structure of diploid and tetraploid Pacific oyster", Aquaculture, 249, pp.487~496 (2005)
- Eudeline B, "Optimization of tet- raploid induction in Pacific oysters, Crassostrea gigas, us- ing first polar body as a natural indicator", Aquaculture, 187, pp.73~84 (2000)
- Eudeline B, "Delayed meiosis and polar body release in eggs of triploid Pacific oysters, Crassostrea gigas, in relation to tetraploid production", J Exp Mar Biol Ecol, 248, pp.151~161 (2000)
- Guo X, "Viable tetraploids in the Pacific oyster (Crassostrea gigas Thunberg) produced by inhibition of polar body I in eggs from triploids", Mol Mar Biol and Biotechnol, 3, pp.42~50 (1994)
- Guo X, "All-triploid Pacific oysters (Crassostrea gigas Thunberg) produced by mating tetraploids and diploids", Aquaculture, 142, pp.149~161 (1996)
- Huang C, "Sperm cryopreservation of green swordtail Xiphophorus helleri, a fish with internal fertilization", Cryobiology, 48, pp.295~308 (2004)
- Hughes JB, "An examination of eggs challenged with cryo- preserved spermatozoa of the American oyster, Crassostrea virginica", Cryobiology, 10, pp.342~344 (1973)
- Hwang SW, "Fertility of male oyster gametes after freeze-thawing", Chinese-American Joint Commission on Rural Reconstruction Fisheries Series, 15, pp.1~5 (1973)
- Jamieson BGM, "With a survey of lophophorate, echi- nodern and photochordate sperm and an account of gamete cryopreservation", pp.319~ (1991), Cambridge University Press
- 고강희, "참돔, Pagrus major 정자의 동결보존에 미치는 희석제와 동해방지제의 효과", 한국어류학회지, 19(2), pp.173~177 (2007), 한국어류학회
- Kuwano K, "Recent advances in marine biotechnol- ogy", pp.23~40 (2000), Science Pubulishers Inc
- McFadzen IRB, "Cryopreservation and Freeze- Drying Protocols", pp.145~149 (1995), Humana Press
- Meryman HT, "Cryoprotective agents", Cryobiology, 8, pp.173~183 (1971)
- Park MS, "Biological and chemical characterization of triploid Pacific oyster (Crassostrea gi- gas) in the waters of Tongyoung, Korea", Bull Fish Res Dev Agency, 55, pp.31~40 (1999)
- 박미선, "굴, Crassostrea gigas 정자의 냉동보존", The Korean Journal of Malacology, 29(3), pp.251~258 (2013), 한국패류학회[DOI]
- Pickett BW, "Preservation of bovine spermatozoa by freezing in straws: A review", J Dairy Sci, 57, pp.1287~1301 (1974)
- Piironen J, "Cryopreservation of sperm from brown trout (Salmon trutta m. lacustris L.) and Arctic charr (Salvelinus alpinus L.)", Aquaculture, 116, pp.275~285 (1993)
- Richardson GF, "Cryo- preservation of Arctic charr, Salvelinus alpinus (L.), semen in various extenders and in three sizes of straw", Aquacult Res, 31, pp.307~315 (2000)
- Richardson GF, "Cryo- preservation of yellowtail flounder (Pleuronectes ferrugin- eus) semen in large straws", Aquaculture, 174, pp.89~94 (1999)
- Sansone G, "Effects of extender composition, cooling rate, and freezing on the motility of sea bass (Dicen- trarchus labrax, L.) spermatozoa after thawing", Cryobiology, 44, pp.229~239 (2002)
- Stanley JG, "Growth of Ameri- can oysters increased by polyploidy induced by blocking meiosisⅠbut not meiosisⅡ", Aquaculture, 37, pp.147~155 (1984)
- Strüssmann CA, "Mo- tility of pejerrey Odontesthes bonariensis spermatozoa", Fish Sci, 60, pp.9~13 (1994)
- Tiersch TR, "Cryopreservation in Aquatic Species", pp.19~26 (2000), World Aquaculture Society
- Wang Z, "Aneuploid Pacific oyster (Crassostrea gigas Thunberg) as incidentals from triploid production", Aquaculture, 173, pp.347~357 (1999)