LPS로 유발된 대식세포의 염증반응에 대한 淸上補下湯의 효과

Abstract

Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti -histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT.Results : Results shows that the CSBHT did not affected cell survival within 101 ng/ml to 105 ng/ml. Especially, 105 ng/ml CSBHT treated cells show 70% deduction of TNF-α gene expression against that of LPS treated group. Furthermore, IL-1β, IL-6, IL-8, IL-10 and TNF-α levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 (TNF-α inhibitor). At protein level, the expression of TNF-α showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ± 87 pg/ml) than in the LPS-treated group (703 ± 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ± 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.