加味潤燥湯이 LPS로 유도된 RAW 264.7 대식세포에서의 항염 효과 연구
Anti-inflammatory Effects of Gamiyunjo-tang on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells
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Objectives : Allergic disease has been well known as an IgE-dependent immunologic response. Recently, interestabout the late inflammatory reaction has grown up as well as early allergic reaction characterized by IgE and mastcell. The purpose of this study was to find the anti-inflammatory effect of Gamiyunjo-tang(GMYJT) in allergicreaction. Methods : The experiment was performed using Raw 264.7 cells pretreated with GMYJT extracts. In this study,we observed the toxicity of cells by MTT analysis and measured the production of LPS-induced NO, PGE₂, IL-1β,IL-6 and TNF-α at a concentration of 50, 100, 200 and 400 ㎍/㎖. Results : No toxicity of GMYJT (50, 100, 200, 400 ㎍/㎖) on RAW 264.7 cells was found after 24 hoursincubation. LPS-induced NO production was reduced after treatment with GMYJT (100, 200, 400 ㎍/㎖)(P<0.05). PGE₂was reduced after treatment with GMYJT (100, 200, 400 ㎍/㎖)(P<0.05). IL-1β did not decrease at any dose. IL-6 decreased at 200, 400 ㎍/㎖(P<0.05). TNF-α production decreased only at 400 ㎍/㎖(P<0.05). Conclusions : These data suggest that GMYJT has anti-inflammatory effects in late allergic reaction.
Abstract
Objectives : Allergic disease has been well known as an IgE-dependent immunologic response. Recently, interestabout the late inflammatory reaction has grown up as well as early allergic reaction characterized by IgE and mastcell. The purpose of this study was to find the anti-inflammatory effect of Gamiyunjo-tang(GMYJT) in allergicreaction. Methods : The experiment was performed using Raw 264.7 cells pretreated with GMYJT extracts. In this study,we observed the toxicity of cells by MTT analysis and measured the production of LPS-induced NO, PGE₂, IL-1β,IL-6 and TNF-α at a concentration of 50, 100, 200 and 400 ㎍/㎖. Results : No toxicity of GMYJT (50, 100, 200, 400 ㎍/㎖) on RAW 264.7 cells was found after 24 hoursincubation. LPS-induced NO production was reduced after treatment with GMYJT (100, 200, 400 ㎍/㎖)(P<0.05). PGE₂was reduced after treatment with GMYJT (100, 200, 400 ㎍/㎖)(P<0.05). IL-1β did not decrease at any dose. IL-6 decreased at 200, 400 ㎍/㎖(P<0.05). TNF-α production decreased only at 400 ㎍/㎖(P<0.05). Conclusions : These data suggest that GMYJT has anti-inflammatory effects in late allergic reaction.
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