降脂通脈飮이 항산화 활성과 高脂血 誘發 白鼠에 미치는 影響
The Effects of Gangzitongmaekeum on Antioxidation Activity and Dietary Hyperlipidemia-induced Mice
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This experiments was performed to determine the effects of Gangzitongmaekeum(降脂通脈飮 : GTE) on antioxidation activity and hyperlipidemia induced by hypercholesterolemic diet in mice. The results obtained were as follows : 1. GTE showed a safety in cytotoxicity and toxicity of human fibroblast cells and liver. 2. GTE showed DPPH scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory effect on reactive oxygen species (ROS). 3. GTE treated mice showed body and liver weight decrease, compared to the hyperlipidemia-induced control group. 4. GTE decreased total cholesterol and LDL cholesterol levels significantly, but HDL cholesterol levels not significantly. 5. GTE decreased triglyceride levels significantly. 6. Glucose levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. 7. Albumin levels in GTE treated mice were similar with the hyperlipidemia-induced control group. 8. The lipophagy in liver compared with the control group tended to be decreased in GTE treated mice. In the change of aorta, the cell was regular and boundary of vessel wall was clear, compared to the hyperlipidemia-induced control group. 9. TBARS levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. 10. The change of SOD and catalase activity significantly increased compared to the hyperlipidemia-induced control group. 11. ACAT mRNA level and HMG-CoA reductase mRNA levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. Conclusion : These results suggest that Gangzitongmaekeum is effective in antioxidation activity and dietary hyperlipidemia-induced mice.
Abstract
This experiments was performed to determine the effects of Gangzitongmaekeum(降脂通脈飮 : GTE) on antioxidation activity and hyperlipidemia induced by hypercholesterolemic diet in mice. The results obtained were as follows : 1. GTE showed a safety in cytotoxicity and toxicity of human fibroblast cells and liver. 2. GTE showed DPPH scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory effect on reactive oxygen species (ROS). 3. GTE treated mice showed body and liver weight decrease, compared to the hyperlipidemia-induced control group. 4. GTE decreased total cholesterol and LDL cholesterol levels significantly, but HDL cholesterol levels not significantly. 5. GTE decreased triglyceride levels significantly. 6. Glucose levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. 7. Albumin levels in GTE treated mice were similar with the hyperlipidemia-induced control group. 8. The lipophagy in liver compared with the control group tended to be decreased in GTE treated mice. In the change of aorta, the cell was regular and boundary of vessel wall was clear, compared to the hyperlipidemia-induced control group. 9. TBARS levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. 10. The change of SOD and catalase activity significantly increased compared to the hyperlipidemia-induced control group. 11. ACAT mRNA level and HMG-CoA reductase mRNA levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. Conclusion : These results suggest that Gangzitongmaekeum is effective in antioxidation activity and dietary hyperlipidemia-induced mice.
참고문헌 (21)
- Fukuzawa K, "Antioxidants", J Act Oxyg Free Rad, 1, pp.55~70 (1990)
- Halliwell B, "Drug antioxidant effects", Drugs, 42, pp.569~605 (1991)
- Hatano T, "Constituents of natural medicines with scavenging effects on active oxygen species-Tannins and related polyphenols-", Natural Medicines, 49, pp.357~363 (1995)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 4617, pp.1198~ (1969)
- 高文社編輯部, "臨床檢査提要", pp.429~431 (1991), 高文社
- 醫學敎育硏修院, "家庭醫學", pp.259~262 (1993), 大學校出版部
- 박영배, "고지혈증", Medical Postgraduates, 31, pp.160~165 (2003)
- 의료보험관리공단, "성인병 예방과 관리", pp.25~ (1993), 의료보험관리공단
- 진귀연, "實用中西醫結合診斷治療學", pp.689~691 (1992), 一中社
- 전국한의과대학 심계내과학교실, "心系內科學", pp.169~173 (2006), 군자출판사
- 鄭遇悅, "韓方臨床病理學", pp.545~ (1998), 永林社
- 鄭南燮, "桑枝가 實驗的 高脂血症의 豫防 및 治療效果에 미치는 影響", pp.90~91 (1996), 慶熙大學校大學院
- 이성두, "地骨皮가 高脂肪食餌로 誘發된 白鼠의 高脂血症에 미치는 影響", 大韓韓方內科學會誌, 19(2), pp.347~366 (1998)
- 최장선, "澤瀉가 白鼠의 高脂血症에 미치는 影響에 關한 硏究", 大韓韓方內科學會誌, 19(2), pp.392~410 (1998)
- 이원구, "淸神導痰湯이 食餌性 高脂血症 動物 病態에 미치는 影響", 석사, pp.2005
- 김정현, "除濕順氣湯이 高脂血症 病態 모델에 미치는 影響", 석사, pp.2005
- 김병철, "丹蔘飮이 高脂血症에 미치는 影響", 大韓韓方內科學會誌, 21(1), pp.126~134 (2000)
- 黃春林, "心血管科專病中醫臨床診治", pp.119~ (2007), 人民衛生出版社
- Sato N, "In vivo and in vitro sevoflurane-induced lipid peroxidation in guinea-pig liver microsomes", Pharmacol Toxicol, 75(6), pp.366~370 (1994)
- Oyanagui Y, "Reevaluation of assay methods and establishment of kit for superoxide dismutase activity", Anal Biochem, 1;142(2), pp.290~296 (1984)
- Aebi H, "Catalase in vitro", Methods Enzymol, 105, pp.121~126 (1984)