rBST 투여가 숫사슴의 혈액 내 IGF-I 함량에 미치는 영향
Effect of Recombinant Bovine Somatotropin Injections on Deer Stag Plasma Insulin-like Growth Factor-I Profiles
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본 연구는 recombinant bovine somatotropin (rBST) 처리가 녹용의 성장과 밀접한 관계가 있는 IGF-I의 분비량에 어떤 영향을 미치는 가를 연구함으로써 rBST 주사(주사량:250mg)에 의한 녹용의 생산성 제고 가능성을 예측하기 위하여 수행하였다. 공시축은 평균 체중이 80.5kg인 숫꽃사슴 3두와 평균 체중이 100.8kg인 레드디어 2두였으며, 실험기간은 2000년 2월부터 6월까지였다.녹용 비성장기(실험 1)의 숫사슴에게 rBST를 처리하지 않은 대조구, rBST 처리1구 및 rBST처리2구의 혈액 내 IGF-I 농도(ng/mL)는 각각 311.1, 306.1 및 279.8로서 처리구간에 차이가 없었으나(p>0.05) rBST를 1주 간격으로 2회 주사한 처리1구와 처리2구 숫사슴의 평균 IGF-I 농도(ng/mL)는 2,920.7로서 rBST를 처리하지 않은 기간에 비하여 8.4배 증가하였다(p<0.01).녹용 성장기(실험 2)의 숫사슴에게 낙각 1주후 rBST를 주사한 처리1구 숫꽃사슴의 평균 IGF-I 농도(ng/mL)가 2,587.6으로서 대조구(461.6)에 비하여 5.6배 증가한 반면 레드디어는 1,976.3으로서 대조구에 비하여 4.3배 증가하였다. 낙각 3주 후의 처리2구 숫사슴에게 rBST주사한 후의 IGF-I 함량(ng/mL)은 대조구(530.4)에 비하여 처리구의 꽃사슴은 3,280 이었고 레드디어는 2,541.6으로서 대조구에 비하여 각각 6.2배와 4.8배 증가하였다.
Abstract
This study was conducted to elucidate the influence of recombinant bovine somatotropin (rBST) on the plasma IGF-I profiles in deer stags. Slow release rBST (250mg each) preparations were injected subcutaneously during the antler’s non-growing period and growing period. It was observed that significant increases in the plasma IGF-I concentrations were occurred right after injections of the rBST irrespective of antler's non-growing period or growing period. When the rBST preparations were injected two times with one week interval during the antler’s non-growing period, the mean level of IGF-I was found to be 2,920.7ng/mL, indicating that the IGF-I concentration increased up to 8.4-folds compared with a control. After two weeks from second shot, however, the IGF-I levels decreased to the level of the control. Similar results were also obtained with both a Sika and a Red deer during the antler’s growing period. Increases in the IGF-I level of up to 5.6-folds with the Sika deer and 4.3-folds with the Red deer were found. Based on the present study, it is likely that the rBST may be used as a possible candidate for an antler’s growth stimulating agent.
참고문헌 (25)
- "Radioimmunoassay for insulin-like growth factor-I solutions to some potential problems and pitfalls", endocri(l), pp.347~357 (1991)
- "Concentration of insulin-like growth factor-I in adult male white-tailed deer associations with serum testosterone morphometrices and age during and after the breeding season", 129, pp.887~895 (2001)
- "Multiple range and multiple F tests", 11, pp.1~42 (1955)
- "Presence of insulin-like growth factor-I receptors and absence of growth hormone receptors in the antler tip", endocri(logy 130), pp.2513~2520 (1992)
- "Receptors for insulin-like growth factor-II in the growing tip of the deer antler", endocri(l), pp.233~241 (1993)
- "of Deer Course for Veterinarians 8", pp.169~180 (1991)
- "Comparative composition of velvet", of deer course for veterinarians 9, pp.26~35 (1992)
- "In Biology of Deer Production Royal Society of New Zealand Bulletin 22", pp.239~250 (1985)
- "A novel method for the extraction of sheep insulin-like growth factors-I and -II from plasma prior to radio- immunoassay", 137, pp.239~245 (1993)
- "Cells cultured from the growing tip of red deer antler express alkaline phosphatase and proliferate in response to insulin-like growth factor-I", endocri(l) (1994)
- "Effects of insulin-like growth factor-I and IGF-II on the growth of antler cells in vitro", endocri(l), pp.461~469 (1994)
- "Antler growth in Roe deer Effects of casting and correlations with growth hormone and insulin-like growth factor-I In The Biology of Deer", pp.505~510 (1992), Springer- Verlag
- "Effects of melatonin implants on insulin-like growth factor-I in male red deer", endocri(l), pp.111~119 (1992)
- "growth and body composition in red deer stag", pp.237~242 (1991)
- "Recent advances in the physiological control of velvet antler growth In The Biology of Deer", pp.471~486 (1992), Springer-Verlag
- "insulin-like growth factors and antler development in red deer", endocri(l), pp.351~360 (1989)
- "Elevated plasma IGF-I levels in stags prevented from growing antlers", endocri(logy), pp.3005~3007 (1988)
- "Antler growth in deer of Deer Course for Veterinarians", pp.155~168 (1991)
- "The effect on winter nutrition and photoperiod on growth of antlers of young red deer In Antler Development in Cervidae", pp.61~71 (1983)
- "The endocrine control of antler growth in red deer stags", pp.7~15 (1984), Lincoln
- "Effects of season and nutrition on growth hormone and insulin- like growth factor-I in male red deer", 137, pp.698~704 (1996)
- "Effects of photoperiod on the cessation of growth during autumn in male red deer and growth hormone and insulin-like growth factor-I secretion", 113, pp.464~477 (1999)
- "The role of photoperiod and nutrition in the seasonal increases in growth and insulin-like growth factor-1 secretion in male red deer", 73, pp.305~311 (2001)
- "사슴사육과 실제" (1994), 도서출판
- "반추동물영양학실험법" (2003), 서울대학교출판부