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敗醬草가 Cationic Bovine Serum Albumin 투여로 유발된 Membranous Nephropathy Mouse Model에 미치는 영향

Effects of the Patriniae Radix Extract on The Membranous Nephropathy Induced by Cationic Bovine Serum Albumin in Mice

조충식|김철중|장선규
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제1호|pp. 212-227

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참고문헌

키워드

membranous nephropathy(MN)cationic bovine serum albumin(cBSA)Patriniae Radix Extract(PRE)
주제분류한의학

초록

Objective : Membranous Nephropathy(MN) is one of the commonest forms of glomerular disease in man and the most frequent cause of the adult idiopathic nephrotic syndrome. Some investigators recommend no treatment, others propose aggressive therapy, including prednisolone plus an immumesuppressant such as chlorambucil or cyclophosphamide. But more effective way to treat MN is not defined yet. This study was to evaluate the effects of Patriniae Radix Extract(PRE) on the MN induced by cBSA in mice. Methods : Mice were divided into 4 groups. The first group named for 'Normal' was injected with saline. The second group named ‘Control’ treated with cBSA(10 ㎎/㎏ i.p) only. The third group named ‘PRE-250’, treated with cBSA(10 ㎎/㎏ i.p) and PRE(250 ㎎/㎏, p.o). The fourth group named ‘PRE-500’ treated with cBSA(10㎎/㎏ i.p) and PRE(500㎎/㎏, p.o). After cBSA and PRE treatment for 4 weeks, we measured change of body weight, 24hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN, creatinine, IgG, IgA, IgM, TNF-α, IL-1β, IFN-γ levels and the mRNA expression of IFN-γ, IL-6, IL-10. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope. Results : The levels of 24 hrs proteinuria and serum triglyceride, BUN, IgG, TNF-α, IL-1β were significantly decreased in both PRE groups. And the level of serum albumin was significantly increased in both PRE groups. The mRNA expression of IL-10 in splenocytes was considerably increased in both PRE group. The mRNA expression of IFN-γ, IL-6 in splenocytes was considerably increased in both PRE group. In histological findings of kidney tissue, thickening of GBM decreased in both PRE groups. Conclusions : The present study suggests that PRE is effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.

Abstract

Objective : Membranous Nephropathy(MN) is one of the commonest forms of glomerular disease in man and the most frequent cause of the adult idiopathic nephrotic syndrome. Some investigators recommend no treatment, others propose aggressive therapy, including prednisolone plus an immumesuppressant such as chlorambucil or cyclophosphamide. But more effective way to treat MN is not defined yet. This study was to evaluate the effects of Patriniae Radix Extract(PRE) on the MN induced by cBSA in mice. Methods : Mice were divided into 4 groups. The first group named for 'Normal' was injected with saline. The second group named ‘Control’ treated with cBSA(10 ㎎/㎏ i.p) only. The third group named ‘PRE-250’, treated with cBSA(10 ㎎/㎏ i.p) and PRE(250 ㎎/㎏, p.o). The fourth group named ‘PRE-500’ treated with cBSA(10㎎/㎏ i.p) and PRE(500㎎/㎏, p.o). After cBSA and PRE treatment for 4 weeks, we measured change of body weight, 24hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN, creatinine, IgG, IgA, IgM, TNF-α, IL-1β, IFN-γ levels and the mRNA expression of IFN-γ, IL-6, IL-10. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope. Results : The levels of 24 hrs proteinuria and serum triglyceride, BUN, IgG, TNF-α, IL-1β were significantly decreased in both PRE groups. And the level of serum albumin was significantly increased in both PRE groups. The mRNA expression of IL-10 in splenocytes was considerably increased in both PRE group. The mRNA expression of IFN-γ, IL-6 in splenocytes was considerably increased in both PRE group. In histological findings of kidney tissue, thickening of GBM decreased in both PRE groups. Conclusions : The present study suggests that PRE is effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.

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