Effects of Opuntia Ficus-indica on Ovalbumin-induced Asthma Model
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Objective : This study was undertaken to investigate effects of Opuntia ficus-indica(OP)extracton asthma murine model. Methods : The number of total cell and eosinophils in BALF and the infiltration of inflammatory cells into lung tissues were determinated by hematoxylin and eosin staining. Results : The number of OVA-induced total cells and eosinophils, a phenomenon of asthma, were decreased by treatment of the animals with OP extract (200 ㎎/㎏) (respectively, p < 0.001 and p<0.01). Furthermore, we show that OP extract reduced the increased immune cell infiltration induced by ovalbumin (p < 0.01). The levels of interleukin (IL)-4 in BAL fluid were measured by Enzyme-linked immunosorbent assay, because the eosinophilia is associated with a T helper (Th) 2 response including IL-4. The level of OVA-induced IL-4 was decreased by OP extract in BAL fluid (p < 0.05). We investigated whether OP extract reduce nitrite (NO) production on lipopolysaccharide (LPS)-stimulated RAW 264.7 sells, because asthma is an inflammatory disease. The level of LPS-induced NO production was decreased by OP extract (50, 100 and 200 mg/ml) on RAW 264.7 cells (respectively, p < 0.05, p<0.01andp<0.05). Conclusions : Our results indicated that OP extract has an inhibition effect on lung eosinophilia of asthma and suggested that OP extract was a therapeutic candidate in the treatment of inflammatory disease, including asthma.
Abstract
Objective : This study was undertaken to investigate effects of Opuntia ficus-indica(OP)extracton asthma murine model. Methods : The number of total cell and eosinophils in BALF and the infiltration of inflammatory cells into lung tissues were determinated by hematoxylin and eosin staining. Results : The number of OVA-induced total cells and eosinophils, a phenomenon of asthma, were decreased by treatment of the animals with OP extract (200 ㎎/㎏) (respectively, p < 0.001 and p<0.01). Furthermore, we show that OP extract reduced the increased immune cell infiltration induced by ovalbumin (p < 0.01). The levels of interleukin (IL)-4 in BAL fluid were measured by Enzyme-linked immunosorbent assay, because the eosinophilia is associated with a T helper (Th) 2 response including IL-4. The level of OVA-induced IL-4 was decreased by OP extract in BAL fluid (p < 0.05). We investigated whether OP extract reduce nitrite (NO) production on lipopolysaccharide (LPS)-stimulated RAW 264.7 sells, because asthma is an inflammatory disease. The level of LPS-induced NO production was decreased by OP extract (50, 100 and 200 mg/ml) on RAW 264.7 cells (respectively, p < 0.05, p<0.01andp<0.05). Conclusions : Our results indicated that OP extract has an inhibition effect on lung eosinophilia of asthma and suggested that OP extract was a therapeutic candidate in the treatment of inflammatory disease, including asthma.
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