소의 모발 멜라닌 색소 함량의 품종별 비교 분석
Comparative Analysis of Melanin Contents in Hairs among Cattle Breeds
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한우와 제주흑우의 모색 특성과 양적 수치화를 위하여 분광광도법적 분석을 수행하였다. 분석 결과 제주흑우의 총 melanin 함량은 같은 흑색 품종인 Holstein과 Angus에 비해 유의적으로 낮았고 한우나 적색 품종 보다는 높은 수준을 보였다(P<0.001). 한우의 총 melanin 함량은 적색 품종에 비해 유의적으로 낮았고 한우×Charolais 교잡종에 비해 높았다(P<0.001). 전체 melanin 중 eumelanin, pheomelanin의 상대적 수준을 분석한 결과 Angus, 제주흑우, Red Holstein과 한우 각각 0.382, 0.359, 0.112와 0.124 이었다. 품종별 모발 melanin 색소 함량 분석결과는 분광광도법적 분석이 소 품종별 모색의 양적 수치화 및 특성 비교에 이용 가능하고 한우와 제주흑우의 주요 모색 관련 유전자 연구의 기초 자료로 유용함을 보여주고 있다.
Abstract
To characterize the colorization patterns of bovine hairs, the melanin contents were quantitatively assayed and compared among cattle breeds. The total melanin levels measured by spectrophotometric assay (A500) from Jeju Black cattle were significantly lower than those from Holstein or Angus with black coat color but significantly higher than those from Hanwoo with yellow coat color or Angus and Holstein with red coat color (P<0.001). The total melanin levels from Hanwoo were significantly lower than those from Red Angus and Red Holstein but significantly higher than those from Hanwoo×Charolais crossbred (P<0.001). The relative ratios of eumelanin to pheomelanin (A650/A500) were 0.382, 0.359, 0.112 and 0.124 in Angus, Jeju Balck cattle, Red Holstein and Hanwoo, respectively. These results show that the spectrophotometric method provides a convenient way to qualitatively characterize hair melanin and may be useful for studying expression of major coat color genes in Hanwoo and Jeju Black cattle.
참고문헌 (24)
- "Relationship of melanin degradation products to actual melanin content: application to human hair", 290, pp.116~125 (2001)
- "Widespread expression of the bovine Agouti gene results from at least three alternative promoters", 18, pp.31~41 (2005)
- "The insertion of a full-length Bos taurus LINE element is responsible for a transcriptional deregulation of the Normande Agouti gene", pp.346~355
- "with the TYR, TYRP1 and DCT transcription levels", 17, pp.337~345 (2004)
- "Eumelanin and pheomelanin concentrations in human epidermis before and after UVB irradiation", 18, pp.220~223 (2005)
- "Genetics, development and evolution of adaptive pigmentation in vertebrates", 97, pp.222~234 (2006)
- "An improved modification of permanganate oxidation of eumelanin that gives a constant yield of pyrrole-2", pp.141~144 (1994)
- "Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals: a comparative review", 16, pp.523~531 (2003)
- "Molecular and developmental genetics of mouse coat color"
- "Red coat color in Holstein cattle is associated with a deletion in the MSHR gene", 7, pp.317~318 (1996)
- "The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination (MSH)", (6), pp.636~639 (1995)
- "Two breed-specific bovine MC1-R alleles in Brown Swiss and Saler Breeds", 84, pp.1768~1771 (2001)
- "Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin", 14, pp.23~31 (2001)
- "Spectrophotometric characterization of eumelanin and pheomelanin in hair", 9, pp.265~270 (1996)
- "with four types of phenotypic pigmentation"
- "A first genotyping assay of french cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (Mc1r)", 32, pp.511~520 (2000)
- "The coding sequence of the ASIP gene is identical in nine wild-type coloured cattle breeds", 122, pp.357~360 (2005)
- "Pigment types of various color genotypes of horses" (1988)
- "Advanced chemical methods in melanin determination", 15, pp.174~183 (2002)
- "제주지방우의 모색분포에 관한 연구", pp.33~45 (1963)
- "제주한우의 모색빈도에 관한 연구", pp.352~255 (1971)
- "한우의 형질과 그 상관에 관한 연구", pp.141~142 (1970)
- "한우, 제주재래흑우, 흑모화우와 갈모화우에서의 MSH receptor(MC1R) 유전자의 유전자형과 빈도 비교", 42, pp.253~260 (2000)
- "소 모색관련 유전자 MC1R의 PCR-RFLP Marker를 이용한 한우육 판별", 42, pp.379~390 (2000)