저산소 노출에 따른 감성돔(Acanthopagrus schlegelii)의생리학적 스트레스 반응
Physiological Stress Responses in Black Seabream Acanthopagrus schlegelii Subjected to Acute Hypoxia
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초록
The black seabream Acanthopagrus schlegelii is an important species for aquaculture in Korea. There are, however, no reports on the physiological responses to hypoxia in this species. The objective of this study was to evaluate the effects of acute hypoxia on the physiological stress responses (plasma cortisol as the primary response, and plasma glucose, hematocrit (Ht), hemoglobin (Hb), sodium, chloride, osmolality, AST, ALT and total protein as the secondary responses) of the fish. The experimental fish were exposed to 0.5 ppm dissolved oxygen (DO) in the seawater via two methods (progressive stepwise decline (Exp.Ⅰ), and direct decline (Exp.Ⅱ)). A highly significant increase was detected in plasma cortisol levels due to the hypoxia treatments, and fish in Exp.Ⅰ had hormone levels that were significantly higher than the fish in Exp.Ⅱ. For plasma glucose, there was no significant difference between the Exp.Ⅰ fish and the control fish, whereas Exp.Ⅱ fish showed significantly higher plasma glucose levels than Exp.Ⅰ fish and control fish. Ht values increased in both hypoxia treatments; however, Hb concentrations increased only in Exp.Ⅰ. Although plasma chloride levels were unaffected by acute hypoxia, plasma sodium and osmolality levels increased in Exp.Ⅰ. Progressive hypoxia (Exp.Ⅰ fish) increased plasma AST, ALT and total protein. These results suggest that the value and direction of changes to the investigated parameters can be used to determine the resistance of black seabream to acute hypoxia
Abstract
The black seabream Acanthopagrus schlegelii is an important species for aquaculture in Korea. There are, however, no reports on the physiological responses to hypoxia in this species. The objective of this study was to evaluate the effects of acute hypoxia on the physiological stress responses (plasma cortisol as the primary response, and plasma glucose, hematocrit (Ht), hemoglobin (Hb), sodium, chloride, osmolality, AST, ALT and total protein as the secondary responses) of the fish. The experimental fish were exposed to 0.5 ppm dissolved oxygen (DO) in the seawater via two methods (progressive stepwise decline (Exp.Ⅰ), and direct decline (Exp.Ⅱ)). A highly significant increase was detected in plasma cortisol levels due to the hypoxia treatments, and fish in Exp.Ⅰ had hormone levels that were significantly higher than the fish in Exp.Ⅱ. For plasma glucose, there was no significant difference between the Exp.Ⅰ fish and the control fish, whereas Exp.Ⅱ fish showed significantly higher plasma glucose levels than Exp.Ⅰ fish and control fish. Ht values increased in both hypoxia treatments; however, Hb concentrations increased only in Exp.Ⅰ. Although plasma chloride levels were unaffected by acute hypoxia, plasma sodium and osmolality levels increased in Exp.Ⅰ. Progressive hypoxia (Exp.Ⅰ fish) increased plasma AST, ALT and total protein. These results suggest that the value and direction of changes to the investigated parameters can be used to determine the resistance of black seabream to acute hypoxia
참고문헌 (51)
- Barton BA, "Physiological changes in fish fromstress in aquaculturewith emphasis on the response and effects of corticosteroids", Ann Rev Fish Dis, 1, pp.3~26 (1991)
- Blasco J, "Fate of plasma glucose in tissues of brown trout in vivo: effects of fasting and glucose loading", Fish Physiol Biochem, 24, pp.247~258 (2001)
- Caldwell CA, "Physiological and haematological responses in rainbow trout subjected to supplemental dissolved oxygen in fish culture", Aquaculture, 126, pp.183~193 (1994)
- Chabot D, "Reduced growth of Atlantic cod in non-lethal hypoxic conditions", J Fish Biol, 55, pp.472~491 (1999)
- Chang YJ, "Black porgy (Acanthopagrus schlegeli) prolactin cDNA sequence: mRNA expression and blood physiological responses during freshwater acclimation", Comp Biochem Physiol B, 147, pp.122~128 (2007)[DOI]
- 최철영, "담수사육 감성돔, Acanthopagrus schlegeli의 수온 상승에 따른 HSP90, HSP70 mRNA의 발현 및 혈액 생리학적 변화", 한국양식학회지, 19(4), pp.315~322 (2006), 한국양식학회
- Choi CY, "Molecular cloning of PEPCK and stress response of black porgy (Acanthopagrusschlegeli) to increased temperature in freshwater and seawater", Gen Comp Endocrinol, 152, pp.47~53 (2007)[DOI]
- Chrousos GP, "The concepts of stress and stress system disorders", J Am Med Assoc, 267, pp.1244~1252 (1992)
- Davis KB, "Management of physiological stress in finfish aquaculture", North Am J Aquaculture, 65, pp.116~121 (2006)
- Davis KB, "Comparison of the cortisol and glucose stress response to acute confinement and resting insulin-like growth factor-I concentrations among white bass, striped bass and sunshine bass", Aquaculture America Book of Abstracts, pp.79~ (2006)
- Delaney MA, "Hypoxic conditions induce Hsp70 production in blood, brain and head kidney of ju-venile Nile tilapia Oreochromis niloticus (L.)", Aquaculture, 236, pp.633~644 (2004)
- Fabbri E, "The role of circulating catecholamines in the regulation of fish metabolism: an overview", Comp Biochem Physiol C, 120, pp.177~192 (1998)
- Herbert N, "The response of Atlantic cod, Gadus morhua, to progressive hypoxia: fish swimming speed and physiological stress", Mar Biol, 147, pp.1403~1412 (2005)
- Hur JW, "Physiological response of the olive flounder, Paralichthys olivaceus, to a series stress during the transportation process", Ichtyol Res, 54, pp.32~37 (2007)
- Iwama GK, "Stress in fishes, In The Physiology of Fishes", pp.319~342 (2006), Taylor and Francis group
- Jentoft S, "Effects of stress on growth, cortisol and glucose levels in non-domesticated Eurasian perch (Perca fluviatilis) and domesticated rainbow trout (Oncorhynchus mykiss)", Comp Biochem Physiol A, 141, pp.353~358 (2005)
- 김영수, "염분변화 속도를 달리한 담수순화 과정에서 강도다리 Platichthys stellatus의 생리학적 반응", 한국양식학회지, 22(1), pp.28~33 (2009), 한국양식학회
- Lai JCC, "Effects of moderate and substantial hypoxia on erythropoietin levels in rainbow trout kidney and spleen", J Exp Biol, 209, pp.2734~2738 (2006)
- Lays N, "Physiological stress responses in spotted wolffish (Anarhichas minor) subjected to acute disturbance and progressive hypoxia", Aquaculture, 295, pp.126~133 (2009)
- Lucas, A, "Physical concepts of bioenergetics, In Bioenergetics of aquatic animals. English edition" (1996), Taylor & Francis
- Martínez-Porchas M, "Cortisol and glucose: Reliable indicators of stress?", Pan Am J Aquat Sci, 4, pp.158~178 (2009)
- Maxime V, "Circulatory and respiratory effects of an hypoxic stress in the Siberian sturgeon", Respir Physiol, 100, pp.203~212 (1995)
- Min BH, "Blood Physiological and Molecular Endocrinological Studies on Osmoregulation of Black Porgy (Acanthopagrus schlegeli; Teleostei) Acclimated in Freshwater", 박사, pp.2006
- 민병화, "담수 및 해수에서 순화 사육한 감성돔, Acanthopagrus schlegeli의 생리상태 비교", 한국양식학회지, 18(1), pp.37~44 (2005), 한국양식학회
- 민병화, "강도다리 Platichthys stellatus 담수 순화시 삼투압조절에 미치는 갑상선호르몬의 영향", 발생과 생식, 13(4), pp.313~320 (2009), 한국발생생물학회
- 민병화, "담수 및 해수사육 감성돔, Acanthopagrus schlegeli의 생리활성과 성장에 미치는 갑상선 호르몬의 효과", 한국양식학회지, 19(3), pp.149~156 (2006), 한국양식학회
- Mommsen TP, "Cortisol in teleosts: dynamic, mechanisms of action, and metabolic regulation", Rev Fish Biol Fish, 9, pp.211~268 (1999)
- Muusze B, "Hypoxia tolerance of Amazon fish Respirometry and energy metabolism of the cichlid Astronotus Ocellatus", Comp Biochem Physiol A, 120, pp.151~156 (1998)
- Nolan DT, "Ambient salinity modulates the response of the tilapia, Oreochromis mossambicus (Peters) to net confinement", Aquaculture, 177, pp.297~309 (1999)
- NFRDI, "Handbook on Oceanography, Marine Environment and Harmful Marine Organisms", pp.108~113 (2009), Hexagondesign
- O’Connor EA, "The effects of acute and chronic hypoxia on cortisol, glucose and lactate concentrations in different populations of three-spined stickleback", Fish Physiol Biochem, 37, pp.461~469 (2011)
- Pan CH, "The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin", J Exp Mar Biol Ecol, 297, pp.107~118 (2003)
- Park MS, "Osmoregulatory ability and stress responses during freshwater adaptation of black porgy (Acanthopagrus schlegeli) treated with exogenous prolactin", Aquac Res, 43, pp.1891~1899 (2012)[DOI]
- Perry SF, "Gas Transport and Gill Function in Water Breathing Fish, In Cardio-Respiratory Control in Vertebrate", pp.5~35 (2009), Springer-Verlag
- Peterson MS, "Hypoxia-induced physiological changes in two mangrove swamp fishes: sheepshead minnow, Cyprinodon variegatus lacepede and sailfin molly, Poecilia latipinna (Lesueur)", Comp Biochem Physiol A, 97, pp.17~21 (1990)
- Pickering AD, "Stress responses and disease resistance in salmonid fish: Effects of chronic elevation of plasma cortisol", Fish Physiol Biochem, 7, pp.253~258 (1989)
- Riche M, "Analysis of refractometry for determining total plasma protein in hybrid striped bass (Morone chrysops×M. saxatilis) at various salinities", Aquaculture, 264, pp.279~284 (2007)
- Rotllant J, "Cortisol and glucose responses after acute stress by net handling in the sparid red porgy previously subjected to crowding stress", J Fish Biol, 51, pp.21~28 (1997)
- Silkin YA, "Effect of hypoxia on physiological-biochemical blood parameters in some marine fish", J Evolut Biochem Physiol, 41, pp.527~532 (2005)
- Smit GL, "The effect of respiratory stress on carp haemoglobin", Comp Biochem Physiol A, 59, pp.369~374 (1978)
- Sun H, "Responses of crucian carp Carassius auratus to long-term exposure to nitrite and low dissolved oxygen levels", Biochem Syst Ecol, 44, pp.224~232 (2012)
- Van der Boon J, "The effects of cortisol administration on intermediary metabolism in teleost fish", Comp Biochem Physiol A, 100, pp.47~53 (1991)
- Van Raaij MTM, "Energy status and free fatty acid patterns in tissues of common carp (Cyprinus carpio, L.) and rainbow trout (Oncorhynchus mykiss, L.) during severe oxygen restriction", Comp Biochem Physiol A, 109, pp.755~767 (1994)
- Vijayan MM, "The stress response and the plasma disappearance of corticosteroid and glucose in a marine teleost, the sea raven", Can J Zool, 72, pp.379~386 (1994)
- Vijayan MM, "Metabolic responses associated with confinement stress in tilapia: the role of cortisol", Comp Biochem Physiol C, 116, pp.89~95 (1997)
- Wedemeyer GA, "Stress and acclimation, In Methods for fish biology", pp.451~489 (1990), American Fisheries Society
- Wells RMG, "Hypoxic responses in a fish from a stable environment: blood oxygen transport in the antarctic fish Pagothenia borchgrevinki", J Exp Biol, 141, pp.97~111 (1989)
- Wendelaar Bonga SE, "The stress response in fish", Physiol Rev, 77, pp.591~625 (1997)
- West TG, "Muscle glucose utilization during sustained swimming in the carp (Cyprinus carpio)", Am J Physiol, 267, pp.R1226~R1234 (1994)
- Wood CM, "Control of red blood cell metabolism in rainbow trout after exhaustive exercise", J Exp Biol, 154, pp.491~507 (1990)
- Yamamoto K, "Direct observations offish spleen by an abdominal window method and its application to exercised and hypoxic yellowtail", Jap J Ichthyol, 31, pp.427~433 (1985)