본문 바로가기
KERD
논문KCI 등재

저산소에 노출된 넙치(Paralichthys olivaceus)의 호흡대사와 혈액의 화학적 변화

Changes in Respiratory Metabolism and Blood Chemistry of Olive Flounder Paralichthys olivaceus Exposed to Hypoxia

1

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류수산학

초록

This experiment investigated changes in metabolic rate (MO2), critical oxygen saturation (Scrit), and blood chemistry of olive flounder Paralichthys olivaceus exposed to progressive hypoxia and returned to normoxic water at 20°C. The normoxic standard metabolic rate (SMR) and routine metabolic rate (RMR) were 69.5-83.9 and 70.2-156.4 mg O2 kg-1h-1, respectively based on fish weight. Scrit was 31.0% dissolved oxygen (DO) at 20°C. After returning the fish to 70% DO following exposure to hypoxia (20% DO), MO2 increased two-fold compared to the normoxic SMR and then decreased into the range of the RMR with time. Blood PO2 and plasma lactate decreased significantly after exposure to hypoxia (20% DO) and then increased as ambient oxygen saturation decreased. Cortisol levels increased as ambient oxygen saturation decreased, but the levels decreased rapidly in the range of the normoxic control when the fish were returned to ambient water with 70% DO. Plasma glucose levels increased when the fish were returned to normoxic water after exposure to a progressively more hypoxic condition.

Abstract

This experiment investigated changes in metabolic rate (MO2), critical oxygen saturation (Scrit), and blood chemistry of olive flounder Paralichthys olivaceus exposed to progressive hypoxia and returned to normoxic water at 20°C. The normoxic standard metabolic rate (SMR) and routine metabolic rate (RMR) were 69.5-83.9 and 70.2-156.4 mg O2 kg-1h-1, respectively based on fish weight. Scrit was 31.0% dissolved oxygen (DO) at 20°C. After returning the fish to 70% DO following exposure to hypoxia (20% DO), MO2 increased two-fold compared to the normoxic SMR and then decreased into the range of the RMR with time. Blood PO2 and plasma lactate decreased significantly after exposure to hypoxia (20% DO) and then increased as ambient oxygen saturation decreased. Cortisol levels increased as ambient oxygen saturation decreased, but the levels decreased rapidly in the range of the normoxic control when the fish were returned to ambient water with 70% DO. Plasma glucose levels increased when the fish were returned to normoxic water after exposure to a progressively more hypoxic condition.

참고문헌 (30)

  1. Barton BA, "Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids", Annu Rev Fish Dis, 1, pp.3~26 (1991)
  2. Dalla Via J, "Influence of long-term hypoxia exposure on the energy metabolism of Solea solea. II. Intermediary metabolism in blood, liver and muscle", Mar Ecol Prog Ser, 111, pp.17~27 (1994)
  3. Fry FEJ., "Fish Physiology", pp.1~98 (1971), Academic Press
  4. S. J. Fu, "Effect of feeding level and feeding frequency on specific dynamic action in Silurus meridionalis", Journal of Fish Biology, 67(1), pp.171~181 (2005), Wiley-Blackwell[DOI]
  5. Herbert NA, "The response of Atlantic cod, Gadus morhua, to progressive hypoxia : fish swimming speed and physiological stress", Mar Biol, 147, pp.1403~1412 (2005)
  6. Jobling M, "A study of some factors affecting rates of oxygen consumption of plaice, Pleuronectes platessa L", J Fish Biol, 20, pp.501~516 (1982)
  7. J. L. Johansen, "The behavioural and physiological response of Atlantic cod Gadus morhua L. to short-term acute hypoxia", Journal of Fish Biology, 68(6), pp.1918~1924 (2006), Wiley-Blackwell[DOI]
  8. Jorgensen JB, "The effect of hypoxia on carbohydrate metabolism in flounder (Platichthys flesus)-I. Utilization of glycogen and accumulation of glycolytic end products in various tissues", Comp Biochem Physiol B, 67, pp.243~248 (1980)
  9. Lennard R, "The effects of hypoxic stress on the fine structure of the flounder heart(Platichthys flesus)", Comp Biochem Physiol A Physiol, 101, pp.723~732 (1992)
  10. Maxime V, "Effect of hypoxia on respiratory physiology of turbot, Scophthalmus maximus", Fish Physiol Biochem, 22, pp.51~59 (2000)
  11. Miller D., "Determination of lethal dissolved oxygen levels for selected marine and estuarine fishes, crustaceans, and a bivalve", Marine Biology, 140(2), pp.287~296 (2002), Springer Nature[DOI]
  12. Noga EJ., "Fish disease: diagnosis and treatment", pp.55~74 (1999), Iowa State University Press
  13. M. A. Peck, "Effects of temperature, body size and feeding on rates of metabolism in young-of-the-year haddock", Journal of Fish Biology, 66(4), pp.911~923 (2005), Wiley-Blackwell[DOI]
  14. Poon WL, "Proceedings of the Sixth International Symposium on Fish Physiology, Toxicology and Water Quality", pp.31~49 (2001), Ecosystems Research Division
  15. Priede IG, "Fish energetics: new perspectives", pp.33~64 (1985), Johns Hopkins University Press
  16. Randall D, "The control of respiration and circulation in fish during exercise and hypoxia", J Exp Biol, 100, pp.275~288 (1982)
  17. Richards JG., "Hypoxia", pp.443~485 (2009), Academy Press
  18. Robertson L, "Plasma cortisol and secondary stress responses of red drum to handling, transport, rearing density, and disease outbreak", Prog Fish-Cult, 49, pp.1~12 (1987)
  19. Schurmann H, "Effects of temperature, hypoxia and activity on the metabolism of juvenile Atlantic cod", J Fish Biol, 50, pp.1166~1180 (1997)
  20. Steffensen JF., "Oxygen consumption of fish exposed to hypoxia: Are they all oxyregulators or are any oxyconformers?", Proceedings of the 9th International Symposium on Fish Physiology, Toxicology and Water Quality (2006)
  21. Brouner CJ, "Ecosystems Research Division", pp.239~250
  22. Steffensen JF, "Some errors in respirometry of aquatic breathers: how to avoid and correct for them", Fish Physiol Bioche, 6, pp.49~59 (1989)
  23. Steffensen JF, "Gill ventilation and O2 extraction during graded hypoxia in two ecologically distinct species of flatfish, the flounder(Platichthys flesus)and the plaice(Pleuronectes platessa)", Environ Biol Fish, 7, pp.157~163 (1982)
  24. Soldatov AA, "The effect of hypoxia on red blood cells of flounder : a morphologic and autoradiographic study", J Fish Biol, 48, pp.321~328 (1996)
  25. Tallqvist M, "Juvenile flounder, Platichthys flesus(L. ), under hypoxia : Effects on tolerance, ventilation rate and predation efficiency", J Exp Mar Biol Ecol, 242, pp.75~93 (1999)
  26. N. H. Thuy, "Critical oxygen tension increases during digestion in the perch Perca fluviatilis", Journal of Fish Biology, 76(4), pp.1025~1031 (2010), Wiley-Blackwell[DOI]
  27. Van Den Thillart G, "Influence of long-term hypoxia exposure on the energy metabolism of Solea solea. I. Critical O2 levels for aerobic and anaerobic metabolism", Mar Ecol Prog Ser, 104, pp.109~117 (1994)
  28. Wendelaar Bonga SE, "The stress response in fish", Physiol Rev, 77, pp.591~625 (1997)
  29. White A, "The effect of physical disturbance, hypoxia and stress hormones on serum components of the plaice, Pleuronectes platessa L", Comp Biochem Physiol A, 93, pp.455~461 (1989)
  30. Zar JH, "Biostatistical Analysis", pp.718~ (1984), Prentice-Hall, Inc

관련 논문AI추천

이 간행물의 다른 논문 (9건)