Docosahexaenoic acid (DHA)를 다량 함유하는 해양미생물의 분리 및 동정
Isolation and Identification of DHA-Rich Marine Microorganism
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Docosahexaenoic acid (DHA, 22:6n-3) and ecosapentaenoic acid (20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. It is thought that DHA has important functions in brain and retinal tissues. Thraustochytrids, a group of marine protists, are capable of heterotrophic growth, and are potential omega-3 producers for industrial use, especially the members of the Schizochytrium and Thraustochytrium genera. The aims of this work were to isolate, identify and screen thraustochytrids from 17 differ- ent locations. Twenty-three isolates were screened for biomass, total fatty acid (TFA) and DHA content. Analysis of the fatty acid methyl esters revealed four distinct clusters biomass ranged from 8.68 - 9.36 g L−1, and lipid and DHA contents ranged from 3.11 - 4.10 g L−1 and 1.05 - 1.93 g L−1 biomass, respectively. B-12 isolates were screened for biomass (9.36 g L−1), TFA (4.10 g L−1) and DHA (47.01%, w/w) content. C-6 isolates were also screened for biomass (8.92 g L−1), TFA (3.30 g L−1) and DHA (49.41%, w/w) content. The 18S rRNA gene sequencing results identified Schizochytrium mangrovei as B-12 and Crypthecodium cohnii as C-6.
Abstract
Docosahexaenoic acid (DHA, 22:6n-3) and ecosapentaenoic acid (20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. It is thought that DHA has important functions in brain and retinal tissues. Thraustochytrids, a group of marine protists, are capable of heterotrophic growth, and are potential omega-3 producers for industrial use, especially the members of the Schizochytrium and Thraustochytrium genera. The aims of this work were to isolate, identify and screen thraustochytrids from 17 differ- ent locations. Twenty-three isolates were screened for biomass, total fatty acid (TFA) and DHA content. Analysis of the fatty acid methyl esters revealed four distinct clusters biomass ranged from 8.68 - 9.36 g L−1, and lipid and DHA contents ranged from 3.11 - 4.10 g L−1 and 1.05 - 1.93 g L−1 biomass, respectively. B-12 isolates were screened for biomass (9.36 g L−1), TFA (4.10 g L−1) and DHA (47.01%, w/w) content. C-6 isolates were also screened for biomass (8.92 g L−1), TFA (3.30 g L−1) and DHA (49.41%, w/w) content. The 18S rRNA gene sequencing results identified Schizochytrium mangrovei as B-12 and Crypthecodium cohnii as C-6.
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