Anti-inflammatory Effect of an Ethanolic Extract of Myagropsis yendoi in Lipopolysaccharide-Stimulated BV-2 Microglia Cells
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Marine brown algae have been identified as a rich source of structurally diverse bioactive compounds. Whether Myagropsis yendoi ethanolic extracts (MYE) inhibit inflammatory responses was investigated using lipopolysaccharide (LPS)-stimulated microglia BV-2 cells. MYE inhibited LPS-induced nitric oxide (NO) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase in BV-2 cells. MYE also reduced the production of pro-inflammatory cytokines in LPSstimulated BV-2 cells. LPS-induced nuclear factor-κB (NF-κB) transcriptional activity and NF-κB translocation into the nucleus were significantly inhibited by MYE treatment through preventing degradation of the inhibitor κB-α. Moreover, MYE inhibited the phosphorylation of AKT, ERK, JNK, and p38 mitogen-activated protein kinase in LPS-stimulated BV-2 cells. These results indicate that MYE is a potential source of therapeutic or functional agents for neuroinflammatory diseases.
Abstract
Marine brown algae have been identified as a rich source of structurally diverse bioactive compounds. Whether Myagropsis yendoi ethanolic extracts (MYE) inhibit inflammatory responses was investigated using lipopolysaccharide (LPS)-stimulated microglia BV-2 cells. MYE inhibited LPS-induced nitric oxide (NO) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase in BV-2 cells. MYE also reduced the production of pro-inflammatory cytokines in LPSstimulated BV-2 cells. LPS-induced nuclear factor-κB (NF-κB) transcriptional activity and NF-κB translocation into the nucleus were significantly inhibited by MYE treatment through preventing degradation of the inhibitor κB-α. Moreover, MYE inhibited the phosphorylation of AKT, ERK, JNK, and p38 mitogen-activated protein kinase in LPS-stimulated BV-2 cells. These results indicate that MYE is a potential source of therapeutic or functional agents for neuroinflammatory diseases.
참고문헌 (26)
- Bozinovski S, "Granulocyte", macrophage-colony-stimulating factor (GM-CSF) regulates lung innate immunity to lipopolysaccharide through Akt, J Biol Chem, pp.2002, 277[DOI]
- Brown GC, "Inflammatory neurodegeneration and mechanisms of microglial killing of neurons", Mol Neurobiol, 41, pp.242~247 (2010)[DOI]
- Cantley LC, "The phosphoinositide 3-kinase pathway", Science, 296, pp.1655~1657 (2002)[DOI]
- Cunningham C, "Microglia and neurodegeneration: the role of systemic inflammation", Glia, 61, pp.71~90 (2012)[DOI]
- Gwon WG, "Hexane fraction from Sargassum fulvellum inhibits lipopolysaccharide-induced inducible nitric oxide synthase expression in RAW 264.7 cells via NF-κB pathways", Am J Chin Med, 41, pp.565~584 (2013)[DOI]
- Herlaar E, "p38 MAPK signalling cascades in inflammatory disease", Mol Med Today, 5, pp.439~447 (1999)[DOI]
- Jana M, "Involvement of phosphatidylinositol 3-kinase-mediated up-regulation of IκBα in anti-inflammatory effect of gemfibrozil in microglia", J Immunol, 179, pp.4142~4152 (2007)
- Jang BC, "Catalase induced expression of inflammatory mediators via activation of NF-κB, PI3K", AKT, p70S6K, and JNKs in BV2 microglia, 2005, pp.17, 625~633
- Janssen-Heininger YMW, "Recent advances towards understanding redox mechanisms in the activation of nuclear factor kappaB", Free Radic Biol Med, 28, pp.1317~1327 (2000)[DOI]
- Joung EJ, "Anti-inflammatory effects of phlorofucofuroeckol B-rich ethyl acetate fraction obtained from Myagropsis myagroides on lipopolysaccharide-stimulated RAW 264.7 cells and mouse edema", Int Immunopharmacol, 14, pp.471~480 (2012)[DOI]
- Joung EJ, "Anti-inflammatory effect of murine macrophages by preventing activation of nuclear factorkappa B", J Immunol, 154, pp.4741~4748 (2012)
- Kim S, "Anti‐inflammatory effect of hexane fraction from Myagropsis myagroides ethanolic extract in lipopolysaccharidestimulated BV‐2 microglial cells", J Pharm Pharmacol, 65, pp.895~906 (2013)[DOI]
- Kim SN, "Sargaquinoic acid and sargahydroquinoic acid from Sargassum yezoense stimulate adipocyte differentiation through PPARα", γ activation in 3T3-L1 cells, 2008, pp.582, 3465~3472
- Lee MS, "Anti-inflammatory activities of an ethanol extract of Ecklonia stolonifera in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells", J. Agric Food Chem, 60, pp.9120~9129 (2012)[DOI]
- Makarov SS, "NF-kappa B in rheumatoid arthritis: a pivotal regulator of inflammation, hyperplasia, and tissue destruction", Arthritis Res, 3, pp.200~206 (2001)
- Marks-Konczalik J, "Cytokine-mediated transcriptional induction of the human inducible nitric oxide synthase gene requires both activator protein 1 and nuclear factor κBbinding sites", J Biol Chem, 273, pp.22201~22208 (1998)
- Meda L, "Activation of microglial cells by β-amyloid protein and interferon-γ", Nature, 374, pp.647~650 (1995)[DOI]
- Minghetti L, "In vivo expression of cyclooxygenase-2 in rat brain following intraparenchymal injection of bacterial endotoxin and inflammatory cytokines", J Neuropathol Exp Neurol, 58, pp.1184~1191 (1999)[DOI]
- Oh YT, "Oleic acid reduces lipopolysaccharide-induced expression of iNOS and COX-2 in BV2 murine microglial cells: possible involvement of reactive oxygen species, p38 MAPK, and IKK", NF-κB signaling pathways, 2009, pp.464, 93~97
- Packard RRS, "Inflammation in atherosclerosis: from vascular biology to biomarker discovery and risk prediction", Clin Chem, 54, pp.24~38 (2008)[DOI]
- Pahl HL, "Activators and target genes of Rel", NF-κB transcription factors, 1999, pp.18, 6853~6866
- Silva AR, "The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures", Cell Biol Toxicol, 24, pp.75~86 (2008)[DOI]
- Skaper SD, "The brain as a target for inflammatory processes and neuroprotective strategies", Ann N Y Acad Sci, 1122, pp.23~34 (2007)[DOI]
- Teismann P, "Pathogenic role of glial cells in Parkinson’s disease", Mov Disord, 18, pp.121~129 (2003)[DOI]
- Xie Q, "Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon gamma and bacterial lipopolysaccharide", J Exp Med, 1776, pp.1779~1784 (1993)[DOI]
- Yang EJ, "Sargachromenol from Sargassum micracanthum inhibits the lipopolysaccharide-induced production of inflammatory mediators in RAW 264.7 macrophages", ScientificWorldJournal (2013)[DOI]