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동과자 에탄올 추출물이 비알코올성 지방간 세포 모델에 미치는 효과

Effects of Ethanol Extract of Benincasa Seeds on the Experimental Cellular Model of Nonalcoholic Fatty Liver Disease

최준용|김소연|권민정|주명수|한창우|김균하
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2012.12|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제33권 제4호|pp. 438-447

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참고문헌

키워드

NAFLDSREBP-1cLXRαBenincasa Seed
주제분류한의학

초록

Objectives : In this study, we investigated the effect and the underlying mechanism of ethanol extract of Benincasa Seeds on a cellular model of non-alcoholic fatty liver disease (NAFLD) established by treating HepG2 cells with palmitate. Methods : We evaluated ethanol extract of Benincasa Seeds (EEBS) for their hepatic lipid-lowering potential in fatty acid overloaded HepG2 cells. After incubation in palmitate containing media with or without EEBS, intracellular neutral lipid accumulation were quantified by Nile Red staining. We also investigated the effect of EEBS on lipogenesis and β-oxidation. LXRα-dependent SREBP-1c activation, expression of lipogenic genes, and expression of β-oxidation related genes were determined with or without pretreatment of EEBS. Results : EEBS significantly attenuated palmitate-induced intracellular neutral lipid accumulation in HepG2 cells. EEBS suppressed fatty acid synthesis by inhibiting LXRα-dependent SREBP-1c activation. EEBS also repressed SREBP-1c mediated induction of lipogenic genes, including ACC, FAS, and SCD-1. But, EEBS had no effect on β-oxidation related CPT-1 and PPARα gene expression. Conclusions : Our study suggestes that EEBS has an efficacy to decrease hepatic lipid accumulation, and this effect was mediated by inhibiting LXRα-SREBP-1c pathway that leads to expression of lipogenic genes and heatic steatosis. Therefore, the Benincasa seeds may be a potential clinical application for treatment of this chronic liver disease.

Abstract

Objectives : In this study, we investigated the effect and the underlying mechanism of ethanol extract of Benincasa Seeds on a cellular model of non-alcoholic fatty liver disease (NAFLD) established by treating HepG2 cells with palmitate. Methods : We evaluated ethanol extract of Benincasa Seeds (EEBS) for their hepatic lipid-lowering potential in fatty acid overloaded HepG2 cells. After incubation in palmitate containing media with or without EEBS, intracellular neutral lipid accumulation were quantified by Nile Red staining. We also investigated the effect of EEBS on lipogenesis and β-oxidation. LXRα-dependent SREBP-1c activation, expression of lipogenic genes, and expression of β-oxidation related genes were determined with or without pretreatment of EEBS. Results : EEBS significantly attenuated palmitate-induced intracellular neutral lipid accumulation in HepG2 cells. EEBS suppressed fatty acid synthesis by inhibiting LXRα-dependent SREBP-1c activation. EEBS also repressed SREBP-1c mediated induction of lipogenic genes, including ACC, FAS, and SCD-1. But, EEBS had no effect on β-oxidation related CPT-1 and PPARα gene expression. Conclusions : Our study suggestes that EEBS has an efficacy to decrease hepatic lipid accumulation, and this effect was mediated by inhibiting LXRα-SREBP-1c pathway that leads to expression of lipogenic genes and heatic steatosis. Therefore, the Benincasa seeds may be a potential clinical application for treatment of this chronic liver disease.

참고문헌 (34)

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