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중만분소환 추출물이 Palmitate로 유발된 비알코올성 지방간 HepG2 cell 모델에 미치는 영향

Effect of Jungmanbunso-hwan Extract on HepG2 Cell Model of Nonalcoholic Fatty Liver Disease Caused by Palmitate

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Objectives: This study was performed to investigate the anti-lipogenic effect and the mechanism of Jungmanbunso-hwan extract (JMBSH) on a cellular model of non-alcoholic fatty liver disease (NAFLD) caused by palmitate in HepG2 cells. Methods: The JMBSH was prepared, andHepG2 cells were treated with various concentrations of JMBSH in order to perform an MTT assay. The HepG2 cells were cultivated in palmitate-containing media with or without extract of JMBSH. The intracellular lipid content in the HepG2 cells was examined. The effects of JMBSH on sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and AMP-activated protein kinase (AMPK) activation in HepG2 cells were measured. Results: JMBSH did not reduce HepG2 cell viability under 1,000 μg/mL. JMBSH considerably decreased intracellular lipid accumulation caused by palmitate in HepG2 cells. JMBSH repressed expression of SREBP-1c, which mediates the induction of lipogenic genes (ACC, FAS, and SCD-1). JMBSH also activated AMPK, which plays animportant role in the regulation of hepatic lipid metabolism. Conclusions: This study suggested that JMBSH relieves hepatic steatosis by repressing SREBP-1c, which mediates the induction of lipogenic genes. The anti-lipogenic effect of JMBSH may also be related to the activation of AMPK. Therefore, JMBSH could potentially be applied to NAFLD treatment after further clinical studies.

Abstract

Objectives: This study was performed to investigate the anti-lipogenic effect and the mechanism of Jungmanbunso-hwan extract (JMBSH) on a cellular model of non-alcoholic fatty liver disease (NAFLD) caused by palmitate in HepG2 cells. Methods: The JMBSH was prepared, andHepG2 cells were treated with various concentrations of JMBSH in order to perform an MTT assay. The HepG2 cells were cultivated in palmitate-containing media with or without extract of JMBSH. The intracellular lipid content in the HepG2 cells was examined. The effects of JMBSH on sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and AMP-activated protein kinase (AMPK) activation in HepG2 cells were measured. Results: JMBSH did not reduce HepG2 cell viability under 1,000 μg/mL. JMBSH considerably decreased intracellular lipid accumulation caused by palmitate in HepG2 cells. JMBSH repressed expression of SREBP-1c, which mediates the induction of lipogenic genes (ACC, FAS, and SCD-1). JMBSH also activated AMPK, which plays animportant role in the regulation of hepatic lipid metabolism. Conclusions: This study suggested that JMBSH relieves hepatic steatosis by repressing SREBP-1c, which mediates the induction of lipogenic genes. The anti-lipogenic effect of JMBSH may also be related to the activation of AMPK. Therefore, JMBSH could potentially be applied to NAFLD treatment after further clinical studies.

참고문헌 (39)

  1. Sourianarayanane A, "Management of non-alcoholic fatty liver disease", Minerva Gastroenterol Dietol, 59(1), pp.69~87 (2013)
  2. Masuoka HC, "Nonalcoholic fatty liver disease: an emerging threat to obese and diabetic individuals", Ann N Y Acad Sci, 1281, pp.106~122 (2013)
  3. Brunt EM, "Nonalcoholic steatohepatitis:a proposal for grading and staging the histological lesions", Am J Gastroenterol, 94(9), pp.2467~2474 (1999)
  4. Influence of dietary intake on non-alcoholic fatty liver disease in Korean, "Jun DW", National Institute of Food and Drug Safety Evaluation, 1~188 (2012)
  5. Beaton MD, "Current treatment options for nonalcoholic fatty liver disease and nonalcoholic steatohepatitis", Can J Gastroenterol, 26(6), pp.353~357 (2012)
  6. Shyangdan D, "Insulin sensitisers in the treatment of non-alcoholic fatty liver disease:a systematic review", Health Technol Assess, 15(38), pp.1~110 (2011)
  7. Professors of Department of Internal Medicine of liver system, National College of Korean Medicine, "Gangye Naegwahak", pp.102~115 (2013), Nado
  8. Lee G, "Nansilbijang", pp.15~ (1994), Euiseongdang
  9. Lee C, "Euhak Ibmun", pp.1808~ (2009), Beop In publishing company
  10. Joo JH, "Dangyesimbeop Buyeo(new edition)", pp.298~ (1993), Dae Seong publishing company
  11. Woo D, "Euhak Jeongjeon", pp.143~144 (1986), Seongbosa
  12. Jin JP, "Manbyeong Hoichun(paginal translation)", pp.391~392 (2007), Beop In publishing company
  13. Heo J, "Dongui Bogam(paginal translation)", pp.1434~ (2010), Dongui Bogam publishing company
  14. Wang A, "Uibang Jiphae", pp.398~400 (1992), Dae Seong publishing company
  15. Lee YU, "The effect of Chungmanbunso-whoan on mouse hepatocyte damages induced by paraquat", Journal of Korean Medicine, 20(3), pp.18~26 (1999)
  16. Repa JJ, "Regulation of mouse sterol regulatory element-binding protein-1c gene(SREBP-1c) by oxysterol receptors, LXRalpha and LXRbeta", Genes Dev, 14(22), pp.2819~2830 (2000)
  17. Zhou G, "Role of AMP-activated protein kinase in mechanism of metformin action", J Clin Invest, 108(8), pp.1167~1174 (2001)
  18. Lin HZ, "Metformin reverses fatty liver disease in obese, leptin-deficient mice", Nat Med, 6(9), pp.998~1003 (2000)
  19. Choi WC, "Nonalcoholic Fatty Liver disease", Inje Medical J, 23(2), pp.217~224 (2002)
  20. Amgulo, P, "Nonalcoholic fatty liver dusease", New England journal Med, 346, pp.1221~1231 (2002)
  21. Sheth SG, "Nonalcoholic steatohepatitis", Ann Intern Med, 126, pp.137~145 (1997)
  22. Park SH, "Nonalcoholic steatohepatitis: Pathogenesis and treatment", Korean J Hepatol, 14(1), pp.12~27 (2008)
  23. 한창우, "비알코올성 지방간 세포 모델에 대한 택사, 산사, 구기자, 울금, 단삼, 인진의 효능 비교", 대한한방내과학회지, 33(4), pp.533~542 (2012), 대한한방내과학회
  24. 장영숙, "적소두가 비알코올성 지방간 질환 세포 모델에 미치는 효과", 동의생리병리학회지, 27(6), pp.802~809 (2013), 한의병리학회
  25. 최준용, "동과자 에탄올 추출물이 비알코올성 지방간 세포 모델에 미치는 효과", 대한한방내과학회지, 33(4), pp.438~447 (2012), 대한한방내과학회
  26. 김소연, "비알코올성 지방간 세포 모델에서 곤포의 효능과 기전 연구", 대한한방내과학회지, 35(2), pp.175~183 (2014), 대한한방내과학회
  27. 최미영, "加味生肝湯이 고지방식이로 유발된 지방간에 미치는 영향", 대한한방내과학회지, 30(2), pp.365~374 (2009), 대한한방내과학회
  28. 신순식, "감비경신환(1)에 의한 고지방식이 비만동물모델에서 체중감량과 지질대사의 조절", 대한본초학회지, 29(1), pp.35~43 (2014), 대한본초학회
  29. 노종성, "고지방식이 유도 비알콜성지방간 마우스 모델에서 六神麯의 脂肪肝 개선효과", 대한본초학회지, 29(2), pp.23~31 (2014), 대한본초학회
  30. Department of Internal Medicine of kidney system, National College of Korean Medicine, "Syngye Naegwahak", pp.89~96 (2011), Gunja publishing company
  31. Gwon GH, "Huang Di Nei Jing (Editing)", pp.21~ (2005), Banryong
  32. Jo HG, "The Bibliographical Study on Causes, Mechanisms and the Chief Virtues on Obesity", Collected papers of Institute of Oriental medicine, 1(2), pp.61~71 (1992)
  33. Bang HJ, "Korean medical consideration of hyperlipidemia", The J of East-West medicines, 20(1), pp.25~36 (1995)
  34. Kola B, "The role of AMP-activated protein kinase in obesity", Front Horm Res, 36, pp.198~211 (2008)
  35. Winder WW, "AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes", Am J Physiol, 277(1 Pt 1), pp.E1~E10 (1999)
  36. Park H, "Coordinate regulation of malonyl-CoA decarboxylase, sn-glycerol-3-phosphate acyltransferase, and acetyl-CoA carboxylase by AMPK in rat tissues in response to exercise", J Biol Chem, 277(36), pp.32571~32577 (2002)
  37. Kawaguchi T, "Mechanism for fatty acid “sparing" effect on glucose-induced transcription:regulation of carbohydrate-responsive elementbinding protein by AMP-activated protein kinase", J Biol Chem, 277(6), pp.3829~3835 (2002)
  38. Muoio DM, "AMP-activated kinase reciprocally regulates triacylglycerol synthesis and fatty acid oxidation in liver and muscle: evidence that sn-glycerol-3-phosphate acyltransferase is a novel target", Biochem J, 338(Pt3), pp.783~791 (1999)
  39. Clarke PR, "Regulation of HMG-CoA reductase: identification of the site phosphorylated by the AMP-activated protein kinase in vitro and in intact rat liver", EMBO J, 9(8), pp.2439~2446 (1990)

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