돼지 FABP3 promoter 부위 내 신규 돌연변이 탐색과 근내지방도와의 연관성 분석
Detection of Novel Mutations in the FABP3 promoter Region and Association Analysis with Intramuscular Fat Content in Pigs
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초록
근내지방도는 양돈산업에 있어서 가장 중요한 경제형질 중의 하나로서, 일반적으로 높은 근내지방도가 양질의 육질을 나타낸다. 근내지방도와 관련된 경제형질좌위에 대한 여러 연구들에 의해서 근내지방도와 연관된 경제형질좌위가 6번 염색체 내 microsatellite marker인 SW71과 SW1881 사이에 존재할 것이라고 보고되었다. 돼지 FABP3은 염색체 상의 위치와 기능적인 측면에서 근내지방도에 영향을 줄 수 있는 후보유전자로 알려져 있으며 많은 연구가 이루어졌다. 본 연구에서 FABP3 promoter 지역의 염기서열 분석을 통해서 두 개의 염기다형성 부위인 g.-114T>C와 g.-158T>G가 새롭게 발견되었으며, 두 부위가 서로 완전히 연관되어 나타남을 확인하였다. 한국재래돼지와 Landrace의 교잡으로 생산된 F2 204 개체를 대상으로 g.-158T>G의 유전자형이 분석되었으며, 성장형질 및 지방축척 형질과의 연관성 분석이 수행되었다. 그 결과, GG 유전자형이 3주령 및 12주령에서의 체중과 1% 수준에서의 유의차를 갖는 높은 부의 상관관계를 보인 반면, 근내지방도와는 5% 수준에서의 유의차로 정의 상관관계를 보였다. 이런 결과는 새로이 발견된 FABP3의 염기다형성이 돼지의 분자육종 분야에서 등지방과는 독립적으로 근내지방도를 향상시키기 위한 유전적 marker로 일부 사용될 수 있을 것을 나타낸다고 사료된다.
Abstract
Intramuscular fat content(IMF) is considered as one of major economic traits in the pig breeding and industry. In general, high IMF results in better meat quality. Several approaches to detect quantitative trait loci(QTL) for IMF indicated a strong possibility of the existence of a QTL related to IMF between the microsatellite marker SW71 and SW1881 on SSC6q. Porcine FABP3 has been considered as a candidate gene affecting IMF due to its physiological roles and position on the pig genome. Two novel mutations, g.-114T>C and g.-158T>G were detected by duplicate sequencing of the porcine FABP3 promoter region. These two mutations were identified as absolute linkage disequilibrium. The g.-158T>G mutation was used for investigating relationships with growth and fat deposition traits. The GG genotype of the g.-158T>G polymorphism showed highly negative effects(P<0.01) on body weights at 3 and 12 weeks of age, and a positive effect(P<0.05) on IMF. However, backfat thickness(BF) and carcass fat(CF) content were not significantly associated with the genotype. The result indicates that the novel mutations, identified in this study, could be utilized as possible genetic markers to improve IMF, independent with BF.
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