본문 바로가기
KERD
논문KCI 등재

수온과 저산소에 따른 조피볼락(Sebastes schlegeli)의호흡대사와 혈액성상의 변화

Changes in metabolic rate and hematological parameters of black rockfish (Sebastes schlegeli) in relation to temperature and hypoxia

김흥윤
수산생명의학회 (Aquatic Life Medical Science)|2021.12|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제34권 제2호|pp. 213-224|DOI http://dx.doi.org/10.7847/jfp.2021.34.2.213

1

조회

0

다운로드

4

인용

0

참고문헌

키워드

HypoxiaOxygen consumptionHematological parameterBlack rockfishCritical oxygen saturation

초록

본 연구는 수온 15, 20 및 25℃에서 조피볼락(Sebastes schlegeli)을 점진적인 저산소 조건에 노출시켜 호흡 대사율이 표준대사율(SMR) 이하로감소하는 산소포화도, Scrit를 평가하고, 수온별 Scrit에 4시간 노출 후 혈액 성상의 변화를 조사하기위하여 실시하였다. 수온 15, 20, 25℃에서 normoxicSMR은 각각 116.5±5.5, 188.6±4.2 및 237.4±6.8 mg O2/kg/hr이었고, 호흡계수(Q10)는 15-20℃ 구간에서는 2.62, 20-25℃에서는 1.58, 그리고 전 수온 범위의 15-25℃에서는 2.04였다. 수온 15, 20 및25℃에서 Scrit는 각각 22.1±1.2, 30.6±1.5 및 41.9±1.4%였다. 수온별 Scrit에 4시간 노출 후 혈액 Ht와 Hb및 혈장 cortisol, glucose, 전해질(Na+, K+, Cl-)과 삼투질 농도는 모든 수온에서 normoxic water의 대조구에 비하여 유의하게 높았다. 수온 15, 20, 25℃의normoxic water에서는 Ht, Hb 및 혈장 glucose 농도는 15℃에 비하여 20℃와 25℃에서, 혈장 cortisol,전해질 및 삼투질 농도는 25℃에서 유의하게 높았다. 수온별 Scrit의 저산소에 노출된 조피볼락은 수온이 높을수록 저산소에 의한 어체의 생리적 스트레스는 가중되는 것으로 나타났다.

Abstract

Experiments were performed to investigate changes in metabolic rate (MO2), critical oxygen saturation(Scrit) and hematological parameters of black rockfish, Sebastes schlegeli exposed to hypoxiaat 15, 20 and 25℃. The MO2 was measured at an interval of 10 min using intermittent-flowrespirometry. The normoxic standard metabolic rate (SMR) was 116.5±5.5, 188.6±4.2 and 237.4±6.8mg O2/kg/hr, and Scrit was 22.1±1.2, 30.6±1.5 and 41.9±1.4% air saturation at 15, 20 and 25℃,respectively. Q10 values were 2.62 between 15 and 20℃, 1.58 between 20 and 25℃, and 2.04 overthe full temperature range. In the investigation of blood (hematocrit and hemoglobin) and biochemicalparameters (plasma cortisol, glucose, electrolyte and osmolality), the rockfish were subjected to Scritfor each temperature during 4 hr. All of hematological parameters of the rockfish exposed to hypoxicwater were significantly higher than those of normoxic control. Moreover, blood and biochemicalparameters of the rockfish maintained to normoxic water showed the tendency of increase with temperature,and were significantly higher at 25C. As a result of this experiment, it was found that physiologicalstress due to hypoxia increased at high temperature.

참고문헌 (49)

  1. Abdel-Tawwab, M., "Fish response to hypoxia stress: growth, physiological, and immunological biomarkers", Fish Physiol. Biochem., 45, pp.997~1013 (2019)
  2. Barton, B.A., "Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids", Annu. Rev. Fish Dis., 1, pp.3~26 (1991)
  3. Brett, J.R., "Fish physiology, vol. 8", pp.599~675
  4. Hoar, W.S., (1979), Academic Press
  5. Buentello, J. A., "Effects of water temperature and dissolved oxygen on daily feed consumption, feed utilization and growth of channel catfish (Ictalurus punctatus)", Aquaculture, 182, pp.339~352 (2000)
  6. Chabot, D., "The determination of standard metabolic rate in fishes", J. Fish Biol., 88, pp.81~121 (2016)
  7. 최혜승, "하절기 조피볼락, Sebastes schlegeli의 대량폐사에 관한 고찰", 한국어병학회지, 22(2), pp.155~162 (2009), 한국어병학회
  8. 도용현, "고수온에서 조피볼락(Sebastes schlegeli)의 산소소비 및 혈액성상 변화", 수산해양교육연구, 28(3), pp.738~745 (2016), 한국수산해양교육학회
  9. Ellis, T., "Cortisol and finfish welfare", Fish Physiol. Biochem., 38, pp.163~188 (2012)
  10. Fernandes, M.N., "Respiratory responses of Oreochromis niloticus (Pisces, Cichlidae) to environmental hypoxia under different thermal conditions", J. Fish Biol., 35, pp.509~519 (1989)
  11. Gaulke, G. L., "Acclimation to a low oxygen environment alters the hematology of largemouth bass (Micropterus salmoides)", Fish Physiol. Biochem., 40, pp.129~140 (2014)
  12. 한지도, "저산소에 노출된 넙치(Paralichthys olivaceus)의 호흡대사와 혈액의 화학적 변화", 한국수산과학회지, 49(1), pp.45~52 (2016), 한국수산과학회
  13. He, W., "Effect of temperature on hypoxia tolerance and its underlying biochemical mechanism in two juvenile cyprinids exhibiting distinct hypoxia sensitivities", Comp. Biochem. Physiol., 187A, pp.232~241 (2015)
  14. Herbert, N.A., "The response of Atlantic cod, Gadus morhua, to progressive hypoxia:fish swimming speed and physiological stress", Mar. Biol., 147, pp.1403~1412 (2005)
  15. 지보영, "수온별 저산소 노출에 따른 조피볼락 (Sebastes schlgeli)의 혈액성상 변화", 환경생물, 33(4), pp.412~418 (2015), 한국환경생물학회
  16. Jia, Y., "Hypoxia tolerance, hematological, and biochemical response in juvenile turbot (Scophthalmus maximus. L)", Aquaculture, 535, pp.1~8 (2021)
  17. Jobling, M., "Temperature tolerance and the final preferendum-rapid methods for the assessment of optimum growth temperatures", J. Fish Biol., 19, pp.439~455 (1981)
  18. Jobling, M., "A study of some factors affecting rates of oxygen consumption of plaice, Pleuronectes platessa L", J. Fish Biol., 20, pp.501~516 (1982)
  19. 정지현, "Biochemical Responses of Juvenile Rockfish (Sebastes schlegeli) to Low Levels of Dissolved Oxygen in Gamak Bay", Ocean Science Journal, 49(3), pp.241~247 (2014), 한국해양과학기술원[DOI]
  20. Wan-SooKim, "Critical Thermal Maximum (CTM) of Cultured Black Rockfish, Sebastes schlegeli", Fisheries and Aquatic Sciences, 6(2) (2003), 한국수산과학회
  21. Kir, M., "Thermal tolerance and standard metabolic rate of juvenile European sea bass (Dicentrarchus labrax, Linnaeus, 1758) acclimated to four temperatures", J. Therm. Biol., 78, pp.209~213 (2018)
  22. Kir, M., "Thermal tolerance and preferred temperature range of juvenile meagre acclimated to four temperatures", J. Therm. Biol., 65, pp.125~129 (2017)
  23. Kita, J., "Temperature preference and tolerance, and oxygen consumption of the marbled rockfish, Sebastiscus marmoratus", Mar. Biol., 125, pp.467~471 (1996)
  24. Lee, Y.S., "Factors affecting outbreaks of Cochlodinium polykrikoides blooms in coastal areas of Korea", Mar. Pollut. Bull., 52, pp.626~634 (2006)
  25. 이덕찬, "2012년 고수온기에 발생한 남동해권역 조피볼락의 대량폐사", 한국어병학회지, 26(3), pp.173~183 (2013), 한국어병학회[DOI]
  26. Maxime, V., "Effect of hypoxia on respiratory physiology of turbot, Scophthalmus maximus", Fish Physiol. Biochem., 22, pp.51~59 (2000)
  27. McBryan, T. L., "Responses to temperature and hypoxia as interacting stressors in fish: Implications for adaptation to environmental change", Integr. Comp. Biol., 53, pp.648~659 (2013)
  28. 오승용, "조피볼락(Sebastes schlegeli)의 산소 소비율에 미치는수온과 체중의 영향", 한국어류학회지, 19(1), pp.1~7 (2007), 한국어류학회
  29. Ott, M. E., "A re-evaluation of the relationship between temperature and the critical oxygen tension in freshwater fishes", Comp. Biochem. Physiol., 67A, pp.337~340 (1980)
  30. Pan, Y. K., "Hypoxia tolerance decreases with body size in red drum Sciaenops ocellatus", J. Fish Biol., 89, pp.1488~1493 (2016)
  31. Pichavant, K., "Comparative effects of long-term hypoxia on growth, feeding and oxygen consumption in juvenile turbot and European sea bass", J. Fish Biol., 59, pp.875~883 (2001)
  32. Rabalais, N. N., "Dynamics and distribution of natural and human-caused hypoxia", Biogeosciences, 7, pp.585~619 (2010)
  33. Randall, D., "The control of respiration and circulation in fish during exercise and hypoxia", J. Exp. Biol., 100, pp.275~288 (1982)
  34. Richards, J. G., "Physiological, behavioral and biochemical adaptations of intertidal fishes to hypoxia", J. Exp. Biol., 214, pp.191~199 (2011)
  35. Schurmann, H., "Lethal oxygen levels at different temperatures and the preferred temperature during hypoxia of the Atlantic cod, Gadus morhua L", J. Fish Biol., 41, pp.927~934 (1992)
  36. Schurmann, H., "Effects of temperature, hypoxia and activity on the metabolism of juvenile Atlantic cod", J. Fish Biol., 50, pp.1166~1180 (1997)
  37. Kyung Suk Seo, "Cochlodinium Red Tide Effects on the Respiration of Abalone,Haliotis discus hannai Ino", ALGAE, 22(3), pp.241~246 (2007), 한국조류학회I
  38. Silkin, Y.A., "Effect of hypoxia on physiological-biochemical blood parameters in some marine fish", J. Evol. Biochem. Physiol., 41, pp.527~532 (2005)
  39. Soivio, A, "The blood oxygen binding properties of hypoxia", J. Comp. Physiol., 136B, pp.83~87 (1980)
  40. Soldatov, A. A., "The effect of hypoxia on red blood cells of flounder: a morphologic and autoradiographic study", J. Fish Biol., 48, pp.321~328 (1996)
  41. Steffensen, J. F., "Some errors in respirometry of aquatic breathers: how to avoid and correct for them", Fish Physiol. Biochem., 6, pp.49~59 (1989)
  42. Steffensen, J.F., "Oxygen consumption of fish exposed to hypoxia: Are they all oxyregulators or are any oxyconformers?", Proceedings of the Ninth International Symposium on Fish Physiology, Toxicology and Water Quality, pp.239~250 (2006), Ecosystems Research Division
  43. Steffensen, J. F., "Gill ventilation and O2 extraction during graded hypoxia in two ecologically distinct species of flatfish, the flounder (Plutichthys flesus) and the plaice (Pleuronectes platessa)", Env. Biol. Fish., 7, pp.157~164 (1982)
  44. Thuy, N. H., "Critical oxygen tension increases during digestion in the perch Perca fluviatilis", J. Fish Biol., 76, pp.1025~1031 (2010)
  45. Tsuchida, S., "The relationship between upper temperature tolerance and final preferendum of Japanese marine fish", J. Therm. Biol., 20, pp.35~41 (1995)
  46. Tsuchida, S., "Experimental study on temperature preference of Japanese marine fish", Rep. Mar. Ecol. Res. Inst., 4, pp.11~66 (2002)
  47. Ultsch, G. R., "Metabolism, critical oxygen tension, and habitat selection in darters (Etheostoma)", Ecology, 59, pp.99~107 (1978)
  48. White, A, "The effect of physical disturbance, hypoxia and stress hormones on serum components of the plaice, Pleuronectes platessa L", Comp. Biochem. Physiol., 93A, pp.455~461 (1989)
  49. Yang, Y., "Histopathological, hematological, and biochemical changes in high-latitude fish Phoxinus lagowskii exposed to hypoxia", Fish Physiol. Biochem., 47, pp.919~938 (2021)

관련 논문AI추천

수온별 저산소 노출에 따른 조피볼락 (Sebastes schlgeli)의 혈액성상 변화도용현, 명정인, 민병화, 박미선, 조재권, 지보영, 황형규한국환경생물학회 · 환경생물2015 · 제33권 제4호조피볼락 Sebastes schlegelii의 생존율, 산소 소비율과 혈액 성상에 미치는 염분의 영향김종관, 명정구, 오승용, 장요순, 최희정한국해양과학기술원 · Ocean and Polar Research2014 · 제36권 제2호저산소에 노출된 넙치(Paralichthys olivaceus)의 호흡대사와 혈액의 화학적 변화김흥윤, 한지도한국수산과학회 · 한국수산과학회지2016 · 제49권 제1호Effects of water temperature changes on oxygen consumption and hematological factors in olive flounder Paralichthys olivace임한규, 한현섭, 허준욱한국수산과학회 · Fisheries and Aquatic Sciences2021 · 제24권 제2호저산소 노출에 따른 감성돔(Acanthopagrus schlegelii)의생리학적 스트레스 반응명정인, 민병화, 박미선, 황형규한국수산과학회 · 한국수산과학회지2013 · 제46권 제6호감성돔(Acanthopagrus schlegelii) 산소 소비율에 미치는 수온과 염분의 영향강필준, 박혜미, 송미해, 오승용, 이근수한국수산과학회 · 한국수산과학회지2024 · 제57권 제5호

이 간행물의 다른 논문 (15건)

강원도의 연어과 어류에서 분리된 IHNV 분리주의 계통분류 및 병원성 분석홍수희, 임종원, 고은호pp. 123-131새로운 betanodavirus 재편성체(reassortant)의어류 치어에 대한 병원성 분석정현도, 김영철pp. 133-140수입 냉동새우에서 검출된 WSSV의 유전학적 근연관계 조사박우성, 김광일, 태원준, 최소원, 김형순, 김민재, 최지영, 백은진pp. 141-147Characterization of rock bream (Oplegnathus fasciatus) fin cells and its susceptibility to different genotypes of megalocytiviruses김광일, 정민아, 김영철, 민준규, 정예진pp. 149-159여윔증상 넙치 및 사육수 내 검출된 점액포자충(Enteromyxum leei and Parvicapsula anisocaudata)의 정량적 분석김예지, 전려진, 이응준, 한지은, 이영준, 정준범pp. 161-168
원문 보기