점망둑(Chasmichthys dolichognathus)의 최종성숙(GVBD)과 배란 유도에 미치는 Prostaglandins의 영향
Effect of Prostaglandins on in vitro Oocyte Final Maturation (GVBD) and Ovulation in the Longchin Goby Chasmichthys dolichognathus
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Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus. Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with PGE2 or PGF2α at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL PGE2 compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL PGE2 or 5, 50, or 500 ng/mL PGF2α). Neither of the prostaglandins induced ovulation at the concentrations tested. These results suggest that GVBD was induced by incubation with 50 ng/mL PGE2.
Abstract
Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus. Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with PGE2 or PGF2α at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL PGE2 compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL PGE2 or 5, 50, or 500 ng/mL PGF2α). Neither of the prostaglandins induced ovulation at the concentrations tested. These results suggest that GVBD was induced by incubation with 50 ng/mL PGE2.
참고문헌 (21)
- 백혜자, "점망둑 (Chasmichthys dolichognathus)의 성숙기 난모세포에서의 C₂₁-스테로이드 생성", 발생과 생식, 12(1), pp.107~112 (2008), 한국발생생물학회
- Baek HJ, "Experimental studies on the machanism of reproductive cycle in the longchin goby Chamichthys dolichognathus", Bull Kor Fish Soc, 18, pp.243~252 (1985)
- Chourasia T, "Role of catechol estrogens on ovarian prostaglandin secretion in vitro in the catfish, Heteropneustes fossilis and possible mechanism of regulation", General Comp Endocrinol, 177, pp.128~142 (2012)[DOI]
- Goetz FW, "Hormonal control of oocyte final maturation and ovulation in fishes", Fish Physiol, 9(Part B), pp.117~170 (1983)
- Goetz FW, "Prostaglandin F and E synthesis by specific tissue components of the brook trout Salvelinus fontinalis ovary", J Exp Zool, 250, pp.196~205 (1989)
- Goetz FW, "In vitro stimulation of germinal vesicle breakdown and ovulation of yellow perch Perca flavescens oocytes : effects of 17α-hydroxy-20β-dihydroprogesterone and prostaglandins", General Comp Endocrinol, 37, pp.273~285 (1979)
- K.P. Joy, "Functional interactions between vasotocin and prostaglandins during final oocyte maturation and ovulation in the catfish Heteropneustes fossilis", General and Comparative Endocrinology, 186, pp.126~135 (2013), Elsevier BV[DOI]
- Kagawa H, "Role of prostaglandin in the control of ovulation in the Japanese eel Anguilla japonica", Fish Sci, 69, pp.234~241 (2003)
- A.L. Lister, "An investigation into the role of prostaglandins in zebrafish oocyte maturation and ovulation", General and Comparative Endocrinology, 159(1), pp.46~57 (2008), Elsevier BV[DOI]
- Esther Lubzens, "Oogenesis in teleosts: How fish eggs are formed", General and Comparative Endocrinology, 165(3), pp.367~389 (2010), Elsevier BV[DOI]
- Mercure F, "Mechanisms of action of free arachidonic acid on ovarian steroid production in the goldfish", General Comp Endocrinol, 102, pp.130~140 (1996)
- Yoshitaka Nagahama, "Regulation of oocyte maturation in fish", Development, Growth & Differentiation, 50, pp.S195~S219 (2008), Wiley[DOI]
- Pang Y, "Gonadotropin and activin enhance maturational competence of oocytes in the zebrafish Danio rerio", Biol Rep, 66, pp.259~265 (2002)
- Patino R, "Ovarian follicle growth, maturation, and ovulation in teleost fish", Fish Physiol Biochem, 26, pp.57~70 (2002)
- Patino R, "Role of arachidonic acid and protein kinase C during maturationinducing hormone-dependent meiotic resumption and ovulation in ovarian follicles of atlantic croaker", Biol Rep, 68, pp.516~523 (2003)
- Sales KJ, "Cyclooxygenase enzymes and prostaglandins in pathology of the endometrium", Rep, 126, pp.559~567 (2003)
- Sorbera LA, "Effects of polyunsaturated fatty acids and prostaglandins on oocyte maturation in a marine teleost, the European sea bass Dicentrarchus labrax", Biol Rep, 64, pp.382~389 (2001)
- Sorensen PW, "F prostaglandins function as potent olfactory stimulants that comprise the postovulatory female sex pheromone in goldfish", Biol Rep, 39, pp.1039~1050 (1988)
- Thomas P, "Upregulation of the maturation-inducing steroid membrane receptor in spotted seatrout ovaries by gonadotropin during oocyte maturation and its physiological significance", Biol Rep, 64, pp.21~29 (2001)
- Wade M, "The control of PGE and PGF production by goldfish testis in vivo", J Exp Zool, 299, pp.108~115 (1993)
- Yonathan Zohar, "Neuroendocrinology of reproduction in teleost fish", General and Comparative Endocrinology, 165(3), pp.438~455 (2010), Elsevier BV[DOI]