누에에서 생산된 뱀장어 재조합 생식소자극호르몬이 암컷 뱀장어(Anguilla japonica)의 성성숙 유도에 미치는 영향
Induction of Sexual Maturation in Female Eels Anguilla japonica by Recombinant Eel Gonadotropin Produced in Silkworm
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초록
This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, withand without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturationin female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinalvesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and rJeFSH∙eCG treatmentsand the control group. However, previous studies did find that rJeLH and rJeLH∙eCG treatments induced GVBD infemale eels. Our in vitro exploration of estradiol-17β (E2) levels in immature ovarian tissues revealed significantlyhigher E2 levels in the group treated with rJeFSH∙eCG 1 μg/mL than in the control group. In contrast, the in vivoexperiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studiesand our own in vitro results have clearly shown that rJeGTH and rJeGTH∙eCG have a positive effect on the sexualmaturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulationtime and activity of these hormones in eels’ bloodstream, modify the structure of the recombinant gene, and implementadditional glycosylation.
Abstract
This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, withand without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturationin female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinalvesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and rJeFSH∙eCG treatmentsand the control group. However, previous studies did find that rJeLH and rJeLH∙eCG treatments induced GVBD infemale eels. Our in vitro exploration of estradiol-17β (E2) levels in immature ovarian tissues revealed significantlyhigher E2 levels in the group treated with rJeFSH∙eCG 1 μg/mL than in the control group. In contrast, the in vivoexperiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studiesand our own in vitro results have clearly shown that rJeGTH and rJeGTH∙eCG have a positive effect on the sexualmaturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulationtime and activity of these hormones in eels’ bloodstream, modify the structure of the recombinant gene, and implementadditional glycosylation.
참고문헌 (27)
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