본문 바로가기
KERD
논문KCI 등재

누에에서 생산된 뱀장어 재조합 생식소자극호르몬이 암컷 뱀장어(Anguilla japonica)의 성성숙 유도에 미치는 영향

Induction of Sexual Maturation in Female Eels Anguilla japonica by Recombinant Eel Gonadotropin Produced in Silkworm

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류수산학

초록

This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, withand without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturationin female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinalvesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and rJeFSH∙eCG treatmentsand the control group. However, previous studies did find that rJeLH and rJeLH∙eCG treatments induced GVBD infemale eels. Our in vitro exploration of estradiol-17β (E2) levels in immature ovarian tissues revealed significantlyhigher E2 levels in the group treated with rJeFSH∙eCG 1 μg/mL than in the control group. In contrast, the in vivoexperiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studiesand our own in vitro results have clearly shown that rJeGTH and rJeGTH∙eCG have a positive effect on the sexualmaturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulationtime and activity of these hormones in eels’ bloodstream, modify the structure of the recombinant gene, and implementadditional glycosylation.

Abstract

This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, withand without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturationin female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinalvesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and rJeFSH∙eCG treatmentsand the control group. However, previous studies did find that rJeLH and rJeLH∙eCG treatments induced GVBD infemale eels. Our in vitro exploration of estradiol-17β (E2) levels in immature ovarian tissues revealed significantlyhigher E2 levels in the group treated with rJeFSH∙eCG 1 μg/mL than in the control group. In contrast, the in vivoexperiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studiesand our own in vitro results have clearly shown that rJeGTH and rJeGTH∙eCG have a positive effect on the sexualmaturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulationtime and activity of these hormones in eels’ bloodstream, modify the structure of the recombinant gene, and implementadditional glycosylation.

참고문헌 (27)

  1. Boëtius J, "Studies of ovarian growth induced by hormone injections in the European and American eel(Anguilla Anguilla L. and Anguilla rostrate LeSueur)", Medd Dan Fisk Havunders New Ser, 3, pp.183~198 (1962)
  2. Claire Cahoreau, "Structure–Function Relationships of Glycoprotein Hormones and Their Subunits’ Ancestors", Frontiers in Endocrinology, 6, pp.1~14 (2015), Frontiers Media SA[DOI]
  3. 최지현, "Molecular Analysis and Bioactivity of Luteinizing Hormone from Japanese Eel, Anguilla japonica, Produced in Silkworm Pupae", Biotechnology and Bioprocess Engineering, 21(3), pp.381~388 (2016), 한국생물공학회
  4. YVES COMBARNOUS, "Molecular Basis of the Specificity of Binding of Glycoprotein Hormones to Their Receptors", Endocrine Reviews, 13(4), pp.670~691 (1992), The Endocrine Society[DOI]
  5. Fortaine M, "Gonadal maturation of female eel Anguilla anguilla and spontaneous emission of eggs in aquarium", CR Acad Sci Paris, 259, pp.2907~2910 (1964)
  6. Gregorio Molés, "Receptor Specificity and Functional Comparison of Recombinant Sea Bass (Dicentrarchus labrax) Gonadotropins (Fsh and Lh) Produced in Different Host Systems1", Biology of Reproduction, 84(6), pp.1171~1181 (2011), Oxford University Press (OUP)[DOI]
  7. Hirohiko Kagawa, "<i>In Vitro<", i> Effects of 17&alpha;-hydroxyprogesterone and 17&alpha;, 20&beta;-dihydroxy-4-pregnen-3-one on Final Maturation of Oocytes at Various Developmental Stages in Artificially Matured Japanese Eel <i>Anguilla japonica<, Fisheries science, pp.1995, 61(6)[DOI]
  8. Hirohiko Kagawa, "Effects of Rearing Period in Seawater on Induced Maturation in Female Japanese Eel <i>Anguilla japonica<", i>, 1998, pp.64(1), 77~82[DOI]
  9. H. Kagawa, "Mechanism of oocyte maturation and ovulation and its application to seed production in the Japanese eel", Fish Physiology and Biochemistry, 39(1), pp.13~17 (2013), Springer Nature[DOI]
  10. Hiroyasu Kamei, "Expression of a biologically active recombinant follicle stimulating hormone of Japanese Eel Anguilla japonica using methylotropic yeast, Pichia pastoris", General and Comparative Endocrinology, 134(3), pp.244~254 (2003), Elsevier BV[DOI]
  11. Kazeto Y., "Ovarian steroidogenesis and the role of sex steroid hormones on ovarian growth and maturation of the Japanese eel", Steroid Biochem Mol Biol, 100, pp.193~201 (2011)[DOI]
  12. Yukinori Kazeto, "Japanese Eel Follicle-Stimulating Hormone (Fsh) and Luteinizing Hormone (Lh): Production of Biologically Active Recombinant Fsh and Lh by Drosophila S2 Cells and Their Differential Actions on the Reproductive Biology1", Biology of Reproduction, 79(5), pp.938~946 (2008), Oxford University Press (OUP)[DOI]
  13. 김대중, "자연산 뱀장어의 인위적인 성숙 유도에 따른 혈중 성호르몬 변동", 한국양식학회지, 19(4), pp.267~274 (2006), 한국양식학회
  14. 김대중, "열목어 재조합 생식선자극호르몬(mt-rGTH)에 의한 암컷 뱀장어의 성성숙 유도", 발생과 생식, 12(3), pp.261~266 (2008), 한국발생생물학회
  15. 김대중, "극동산 뱀장어, Anguilla japonica 자성화에 미치는 estradiol-17β 유도 효과", 생명과학회지, 23(8), pp.998~1003 (2013), 한국생명과학회[DOI]
  16. Dae-Jung Kim, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica", General and Comparative Endocrinology, 233, pp.8~15 (2016), Elsevier BV[DOI]
  17. Klein J, "Development and charaterization of a long-acting recombinant hFSH agonist", Human Reprod, 18, pp.50~56 (2003)[DOI]
  18. Makito Kobayashi, "Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae", General and Comparative Endocrinology, 167(3), pp.379~386 (2010), Elsevier BV[DOI]
  19. S. Legardinier, "Biological activities of recombinant equine luteinizing hormone", chorionic gonadotropin (eLH, Journal of Molecular Endocrinology, pp.2005, 34(1)[DOI]
  20. Maeda S, "Insect cell biotechology", pp.1~31 (1994), CRC Press
  21. Min KS, "Biological activities of tethered equine chorionic gonadotropin (eCG) and its deglycosylated mutants", J Reprod Dev, 50, pp.297~304 (2004)[DOI]
  22. Kiichiro YAMAMOTO, "Cytological changes in the adenohypophysis of fresh-water cultivated male Japanese eels, Anguilla japonica, induced to maturation by transfer to sea water and Synahorin injection", NIPPON SUISAN GAKKAISHI, 39(6), pp.585~594 (1973), Japanese Society of Fisheries Science[DOI]
  23. Nagahama Y., "Endocrine regulation of gametogenesis in fish", Int J Dev Biol, 38, pp.217~229 (1994)
  24. Shin DH, "Biochemical and histological studies on egg quality in the Japanese eel Anguilla japonica", Division of Marine Biosciences, pp.2004
  25. P. Swanson, "Biochemistry and physiology of fish gonadotropins", Fish Physiology and Biochemistry, 28(1-4), pp.53~59 (2003), Springer Nature[DOI]
  26. Vischer HF, "Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor", Journal of Molecular Endocrinology, 31(1), pp.133~140 (2003), Bioscientifica[DOI]
  27. K. YAMAMOTO, "Sexual maturation of Japanese eel and production of eel larvae in the aquarium", Nature, 251(5472), pp.220~222 (1974), Springer Nature[DOI]

관련 논문AI추천

Characterization of ovarian culture in vitro and sex steroids in vivo by recombinant eel gonadotropin treatments in the eel Anguilla japonica김대중, 김신권, 김정현, 민관식, 백혜자, Dae-Geun Kim한국수산과학회 · Fisheries and Aquatic Sciences2022 · 제25권 제1호열목어 재조합 생식선자극호르몬(mt-rGTH)에 의한 암컷 뱀장어의 성성숙 유도김대중, 박우동, 배준영, 손맹현, 손영창, 윤성종, 한창희한국발생생물학회 · 발생과 생식2008 · 제12권 제3호Signal Transduction of Eel Luteinizing Hormone Receptor (eelLHR) and Follicle Stimulating Hormone Receptor (eelFSHR) by Recombinant Equine Chorionic Gonadotropin (rec-eCG) and Native eCG강명화, 민관식, Dae-Jung Kim, Munkhzaya Byambaragchaa, So-Yun Lee한국발생생물학회 · 발생과 생식2018 · 제22권 제1호Ovarian Development of Conger Eel in Korea, Conger myriaster, in Captivity이원교, Chung-Kug Park, Joon-Taek Park, Kyoung-Sik Lee, Kyoung-Woo Lee, Se-Un Ki한국발생생물학회 · 발생과 생식2021 · 제25권 제4호겨울철 사육조건이 양식산 뱀장어 Anguilla japonica의 인위적인 성성숙 유도 및 번식에 미치는 영향김대중, 김이청, 박미선, 손맹현, 이정의, 최용기, 허윤성한국발생생물학회 · 발생과 생식2009 · 제13권 제1호Effects of Steroid Hormones on In Vitro GVBD and Oocyte Steroidogenesis in Blacktip Grouper, Epinephelus fasciatus김형배, 백혜자, 이영돈, 이치훈(Chi Hoon Lee), In Joon Hwang, Sang Jun Choi, Seol Ki Kim한국발생생물학회 · 발생과 생식2012 · 제16권 제1호

이 간행물의 다른 논문 (26건)